Aged Oolong Tea Reduces High-Fat Diet-Induced Fat Accumulation and Dyslipidemia by Regulating the AMPK/ACC Signaling Pathway
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of EAOTs
2.3. Determination of Ingredients in EAOTs
2.4. Animals
2.5. Diet-Induced Obesity
2.6. EAOT Treatment
2.7. Lee’s Index
2.8. Serum Chemistry Analysis
2.9. Histological Examination
2.10. Western Blotting Analysis
2.11. Immunohistochemistry (IHC)
2.12. Statistical Analysis
3. Results
3.1. The Ingredients of EAOTs
3.2. Body Weight, Food and Water Intake, and Lee’s Index
3.3. EAOTs Attenuate Fatty Liver and Adiposity in HFD-Induced Obese Mice
3.4. Effects of EAOTs on Serum Levels in HFD-Induced Obesity Mice
3.5. Effect of EAOTs on Accumulation of Lipid Droplets in Liver and Epididymal Fat
3.6. EAOTs Activate AMPK and ACC Phosphorylation
3.7. EAOTs Activate CPT-1 Expression and Inhibit FAS Expression
3.8. EAOTs Inhibit iNOS and TNF-α Expression
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample | Water (%) | Free Amino Acids (%) | Total Soluble Sugar (%) | Tea Polyphenols (%) |
---|---|---|---|---|
2016 | 0.07 ± 0.01 a | 5.13 ± 0.03 a | 7.08 ± 0.25 a | 39.7 ± 0.3 a |
2006 | 0.08 ± 0.01 a | 3.99 ± 0.19 b | 8.18 ± 0.08 b | 46.5 ± 0.6 b |
1996 | 0.06 ± 0.01 a | 3.81 ± 0.04 b | 8.39 ± 0.08 b | 34.0 ± 0.5 c |
Group | Liver/Weight (%) | Kidney/Weight (%) | Epididymal Fat/Weight (%) | Intestinal Fat/Weight (%) | Pararenal Fat/Wright (%) | White Fat/Weight (%) |
---|---|---|---|---|---|---|
Control | 44.5 ± 0.1 a | 13.0 ± 1.0 a | 1.30 ± 0.46 a | 5.14 ± 2.43 a | 4.15 ± 1.98 a | 9.31 ± 4.32 a |
Model | 45.2 ± 0.5 a | 9.39 ± 0.88 b | 5.35 ± 0.29 b | 23.9 ± 5.3 b | 30.6 ± 3.3 b | 56.3 ± 6.1 b |
2016 | 36.4 ± 0.1 b | 11.9 ± 0.9 a | 3.02 ± 1.16 c | 10.4 ± 5.4 a | 16.4 ± 8.0 c | 24.7 ± 16.3 c |
2006 | 37.0 ± 0.1 b | 12.4 ± 1.5 a | 2.77 ± 0.75 c | 7.73 ± 3.76 a | 11.8 ± 6.1 a,c | 16.4 ± 5.6 a,c |
1996 | 37.0 ± 0.1 b | 12.4 ± 1.6 a | 2.91 ± 1.07 c | 9.20 ± 4.65 a | 12.8 ± 6.5 c | 19.7 ± 9.3 a,c |
Group | TG (mmol/L) | TC (mmol/L) | HDL-C (mmol/L) | LDL-C (mmol/L) |
---|---|---|---|---|
Control | 1.41 ± 0.30 a | 4.18 ± 0.58 a | 2.78 ± 0.37 a | 0.55 ± 0.12 a |
Model | 1.95 ± 0.34 b | 9.25 ± 1.30 b | 2.26 ± 0.24 a | 1.46 ± 0.33 b |
2016 | 1.45 ± 0.22 a | 6.20 ± 0.59 c | 4.03 ± 0.44 b | 1.00 ± 0.11 c |
2006 | 1.13 ± 0.26 a | 5.74 ± 0.46 c | 3.80 ± 0.36 b | 0.86 ± 0.10 c |
1996 | 1.19 ± 0.24 a | 6.42 ± 0.84 c | 4.21 ± 0.58 b | 0.97 ± 0.24 c |
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Yuan, E.; Duan, X.; Xiang, L.; Ren, J.; Lai, X.; Li, Q.; Sun, L.; Sun, S. Aged Oolong Tea Reduces High-Fat Diet-Induced Fat Accumulation and Dyslipidemia by Regulating the AMPK/ACC Signaling Pathway. Nutrients 2018, 10, 187. https://doi.org/10.3390/nu10020187
Yuan E, Duan X, Xiang L, Ren J, Lai X, Li Q, Sun L, Sun S. Aged Oolong Tea Reduces High-Fat Diet-Induced Fat Accumulation and Dyslipidemia by Regulating the AMPK/ACC Signaling Pathway. Nutrients. 2018; 10(2):187. https://doi.org/10.3390/nu10020187
Chicago/Turabian StyleYuan, Erdong, Xuefei Duan, Limin Xiang, Jiaoyan Ren, Xingfei Lai, Qiuhua Li, Lingli Sun, and Shili Sun. 2018. "Aged Oolong Tea Reduces High-Fat Diet-Induced Fat Accumulation and Dyslipidemia by Regulating the AMPK/ACC Signaling Pathway" Nutrients 10, no. 2: 187. https://doi.org/10.3390/nu10020187