Magnetic-Assisted Cell Alignment within a Magnetic Nanoparticle-Decorated Reduced Graphene Oxide/Collagen 3D Nanocomposite Hydrogel
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of M-rGO
2.3. Preparation of Collagen and M-rGO/Collagen Composites
2.4. Cell Culture
2.5. Live/Dead Cell Assay
2.6. Immunofluorescence Staining
2.7. Measurement of Cell Orientation
2.8. Calcium Imaging
3. Results
3.1. Synthesis and Characterization of M-rGO
3.2. Biocompatibility of M-rGO/Collagen Scaffolds
3.3. Orientation of Cells within M-rGO/Collagen Scaffolds
3.4. Differentiation of SH-SY5Y Cells within M-rGO/Collagen Scaffolds
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Santhosh, M.; Choi, J.-H.; Choi, J.-W. Magnetic-Assisted Cell Alignment within a Magnetic Nanoparticle-Decorated Reduced Graphene Oxide/Collagen 3D Nanocomposite Hydrogel. Nanomaterials 2019, 9, 1293. https://doi.org/10.3390/nano9091293
Santhosh M, Choi J-H, Choi J-W. Magnetic-Assisted Cell Alignment within a Magnetic Nanoparticle-Decorated Reduced Graphene Oxide/Collagen 3D Nanocomposite Hydrogel. Nanomaterials. 2019; 9(9):1293. https://doi.org/10.3390/nano9091293
Chicago/Turabian StyleSanthosh, Mallesh, Jin-Ha Choi, and Jeong-Woo Choi. 2019. "Magnetic-Assisted Cell Alignment within a Magnetic Nanoparticle-Decorated Reduced Graphene Oxide/Collagen 3D Nanocomposite Hydrogel" Nanomaterials 9, no. 9: 1293. https://doi.org/10.3390/nano9091293