Nanoformulation of Polyphenol Curcumin Enhances Cisplatin-Induced Apoptosis in Drug-Resistant MDA-MB-231 Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Gemini-Cur and Cisplatin Have a Synergistic Toxic Effect on MDA-MB-231 Cells
2.2. Morphological Visualization of Apoptosis
2.3. Gemini-Cur/Cis Modulates Cell Cycle Distribution in MDA-MB-231 Cells
2.4. Annexin V-FITC/PI Assay Confirmed Apoptosis in MDA-MB-231 Treated Cells
2.5. Expression of BAX, BCL-2 Genes Are Modulated in Treated Cells
3. Discussion
4. Materials and Methods
4.1. Reagents and Cell Culture
4.2. Cell Viability Assay
4.3. Analysis of Synergistic Cytotoxicity
4.4. Visualization of Apoptosis by Hoechst Staining
4.5. Cell Cycle Analysis by Flow Cytometry
4.6. Annexin V FITC/PI Assay
4.7. Expression Studies by Real-Time PCR and Western Blotting
4.7.1. Real-Time PCR
4.7.2. Western Blotting
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Genes | Primers | Size (bp) |
---|---|---|
β-actin | F: 5′-TGCCCATCTACGAGGGGTATG-3′ R: 5′-CTCCTTAATGTCACGCACGATTTC-3′ | 155 |
BAX | F: 5′-GCAAACTGGTGCTCAAGG-3′ R: 5′-ACTCCCGCCACAAAGA-3′ | 236 |
BCL-2 | F: 5′-TGGGAAGTTTCAAATCAGC-3′ R: 5′-GCATTCTTGGACGAGGG-3′ | 298 |
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Karami, P.; Othman, G.; Housein, Z.; Salihi, A.; Hosseinpour Feizi, M.A.; Azeez, H.J.; Babaei, E. Nanoformulation of Polyphenol Curcumin Enhances Cisplatin-Induced Apoptosis in Drug-Resistant MDA-MB-231 Breast Cancer Cells. Molecules 2022, 27, 2917. https://doi.org/10.3390/molecules27092917
Karami P, Othman G, Housein Z, Salihi A, Hosseinpour Feizi MA, Azeez HJ, Babaei E. Nanoformulation of Polyphenol Curcumin Enhances Cisplatin-Induced Apoptosis in Drug-Resistant MDA-MB-231 Breast Cancer Cells. Molecules. 2022; 27(9):2917. https://doi.org/10.3390/molecules27092917
Chicago/Turabian StyleKarami, Parastoo, Goran Othman, Zjwan Housein, Abbas Salihi, Mohammad Ali Hosseinpour Feizi, Hewa Jalal Azeez, and Esmaeil Babaei. 2022. "Nanoformulation of Polyphenol Curcumin Enhances Cisplatin-Induced Apoptosis in Drug-Resistant MDA-MB-231 Breast Cancer Cells" Molecules 27, no. 9: 2917. https://doi.org/10.3390/molecules27092917