Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation
Abstract
:1. Introduction
2. Results
2.1. Effect of Nitrogen Availability on Various Physiological Characteristics of Phaeodactylum tricornutum
2.2. Semi-Quantification of FCP Complexes in the Thylakoid Membrane of Day 5 Samples
2.3. Transcriptome Analysis of Phaeodactylum tricornutum under Varying Nitrogen Conditions
2.4. Combination of Light and Nitrogen Supply to Enhance Fucoxanthin Production
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. P. tricornutum Cultivation
4.3. Growth Determination and Biomass Production
4.4. Quantification of Primary Photosynthetic Pigments
4.4.1. Fucoxanthin Quantification with HPLC-UV-DAD Analysis
4.4.2. Quantification of Chlorophylls with a UV/Vis Spectrophotometer
4.5. Total Nitrogen Content in P. tricornutum Culture Medium
4.6. Semi-Quantification of Fucoxanthin–Chlorophyll a/c-Binding Proteins
4.6.1. Thylakoid Membrane Isolation
4.6.2. Proteomic Analysis with SDS-PAGE Coupled with LC-MS Analysis
4.7. RNA Sequencing Analysis of Day 5 Samples
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Accession No. | Alternate ID | LN | NN | HN |
---|---|---|---|---|
B7FRW5 | LHCF1 | 2857 ± 273 c | 11,243 ± 1525 b | 21,399 ± 1584 a |
B7FRW4 | LHCF2 | 2844 ± 328 c | 12,718 ± 1318 b | 22,923 ± 1385 a |
B7FRW2 | LHCF3/4 | 13,016 ± 1422 c | 39,244 ± 945 b | 67,185 ± 3149 a |
B7GBK7 | LHCF5 | 3124 ± 433 c | 16,442 ± 2394 b | 31,459 ± 1594 a |
B7G5S7 | LHCF6 | 2270 ± 267 c | 7624 ± 1040 b | 15,851 ± 1660 a |
B7G6Y1 | LHCF8 | 5580 ± 1122 c | 18,586 ± 1326 b | 32,556 ± 2839 a |
B7G955 | LHCF9 | 401 ± 69 c | 1230 ± 399 b | 2804 ± 1421 a |
B7G5B6 | LHCF10 | 3297 ± 718 c | 14,299 ± 1525 b | 26,277 ± 1850 a |
B7GBK6 | LHCF11 | 4659 ± 1123 c | 15,459 ± 669 b | 25,301 ± 1245 a |
B7GCA2 | LHCF12 | 3484 ± 984 c | 11,945 ± 791 b | 19,205 ± 1901 a |
B7G871 | LHCF13 | 254 ± 46 c | 843 ± 279 b | 1646 ± 317 a |
B5Y5L4 | LHCF14 | 1415 ± 231 c | 4813 ± 580 b | 9206 ± 460 a |
B7FV42 | LHCF16 | 80 ± 0 c | 351 ± 161 b | 610 ± 381 a |
B7GC99 | LHCF17 | 334 ± 61 c | 1124 ± 219 b | 1890 ± 761 a |
B7FUM6 | LHCR1 | 587 ± 101 c | 1124 ± 122 b | 1585 ± 211 a |
B7FSP4 | LHCR3 | 935 ± 23 c | 2846 ± 105 b | 4938 ± 317 a |
B7FQE1 | LHCR4 | 267 ± 23 c | 1230 ± 61 b | 1890 ± 381 a |
B7FQE0 | LHCR12 | 1695 ± 311 c | 5059 ± 422 b | 7682 ± 1018 a |
B7G502 | LHCR13 | 854 ± 46 c | 2846 ± 658 b | 4694 ± 1598 a |
B7G503 | LHCR14 | 1268 ± 228 c | 5973 ± 896 b | 9511 ± 2420 a |
B7FYL0 | LHCX1 | 240 ± 40 c | 773 ± 265 b | 1280 ± 484 a |
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Truong, T.Q.; Park, Y.J.; Winarto, J.; Huynh, P.K.; Moon, J.; Choi, Y.B.; Song, D.-G.; Koo, S.Y.; Kim, S.M. Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation. Mar. Drugs 2024, 22, 93. https://doi.org/10.3390/md22020093
Truong TQ, Park YJ, Winarto J, Huynh PK, Moon J, Choi YB, Song D-G, Koo SY, Kim SM. Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation. Marine Drugs. 2024; 22(2):93. https://doi.org/10.3390/md22020093
Chicago/Turabian StyleTruong, To Quyen, Yun Ji Park, Jessica Winarto, Phuong Kim Huynh, Jinyoung Moon, Yeong Bin Choi, Dae-Geun Song, Song Yi Koo, and Sang Min Kim. 2024. "Understanding the Impact of Nitrogen Availability: A Limiting Factor for Enhancing Fucoxanthin Productivity in Microalgae Cultivation" Marine Drugs 22, no. 2: 93. https://doi.org/10.3390/md22020093