The Forkhead Box Gene, MaSep1, Negatively Regulates UV- and Thermo-Tolerances and Is Required for Microcycle Conidiation in Metarhizium acridum
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Cultivations
2.2. Bioinformatic Analyses
2.3. Gene Disruption and Complementation
2.4. Conidial Germination and Conidiation Capacity Assays
2.5. Stress Tolerance Assays
2.6. DNA Damage and Repair
2.7. Pathogenicity Assays
2.8. qRT-PCR and RNA-Seq
2.9. Data Analyses
3. Results
3.1. Features of MaSep1
3.2. Deletion of MaSep1 Affected Conidial Germination and Conidial Production, but Not Virulence
3.3. Deletion of MaSep1 Affects Multiple Stress Tolerances in M. acridum
3.4. MaSep1 Is Required for Microcycle Conidiation of M. acridum
3.5. Identification of the DEGs Regulated by MaSep1 Using RNA-Seq
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Song, T.; Li, C.; Jin, K.; Xia, Y. The Forkhead Box Gene, MaSep1, Negatively Regulates UV- and Thermo-Tolerances and Is Required for Microcycle Conidiation in Metarhizium acridum. J. Fungi 2024, 10, 544. https://doi.org/10.3390/jof10080544
Song T, Li C, Jin K, Xia Y. The Forkhead Box Gene, MaSep1, Negatively Regulates UV- and Thermo-Tolerances and Is Required for Microcycle Conidiation in Metarhizium acridum. Journal of Fungi. 2024; 10(8):544. https://doi.org/10.3390/jof10080544
Chicago/Turabian StyleSong, Tiantian, Chan Li, Kai Jin, and Yuxian Xia. 2024. "The Forkhead Box Gene, MaSep1, Negatively Regulates UV- and Thermo-Tolerances and Is Required for Microcycle Conidiation in Metarhizium acridum" Journal of Fungi 10, no. 8: 544. https://doi.org/10.3390/jof10080544