Differential Expression of LMNA/C and Insulin Receptor Transcript Variants in Peripheral Blood Mononuclear Cells of Leukemia Patients
Abstract
:1. Introduction
2. Materials and Methods
3. Results
3.1. Demographic Data of Subjects
3.2. Differential Expression of LMNA/C mRNA Expression in PBMC
3.3. Inhibition of INSR (IR-A) mRNA Expression in PBMC
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer. | Sense (5′→3′) | Anti Sense (5′→3′) | Probe | Accession Number |
---|---|---|---|---|
LMNA | TGACTGTGGTTGAGGACGAC | GACACTGGAGGCAGAAGAGC | CGCTGAGTACAACCT | NM_170707.3 |
LMNC | GTGGAAGGCACAGAACACCT | GCGGCGGCTACCACTCAC | AGATGACCTGCTCCATCACC | NM_005572.3 |
LMNAΔ10 | AACTCCACTGGGGAAGGCTCC | GCTCCTGAGCCGCTGGCAGA | AGTACAACCTGCGCTCGCGC | NM_170708.3 |
LMNAΔ50 | GCGTCAGGAGCCCTGAGC | GACGCAGGAAGCCTCCAC | AGCATCATGTAATCTGGGACCT | NM_001282626.1 |
INSR (IR-A) | TATCCGGAACAACCTCACTA | GGAAGAGCAGCAAGTAATCA | CTCTGTCATCGAAGGACACTTG | NM_001079817 |
INSR (IR-B) | AGGAGTCCTCGTTTAGGAAG | AGGAAGTGTTGGGGAAAG | AGAAAAACCTCTTCAGGCACTG | NM_000208 |
Ubiquitin C | ACTACAACATCCAGAAAGAGTCCA | CCAGTCAGGGTCTTCACGAAG | CCCACCTCTGAGACGGAGCACCAG | NM_021009.6 |
RPL13 | AACAAGTTGAAGTACCTGGCTTTC | TGGTTTTGTGGGGCAGCATA | CGCAAGCGGATGAACACCAACCCT | NM_000977.3 |
Gender | Age (years) | BMI kg/m2 | |
---|---|---|---|
Control | 14M, 18F | 31.9 ± 2.18 | 32.1 ± 2.0 |
AML | 9M, 8F | 48.2 ± 5.18 * | 26.4 ± 1.28 |
CML | 4M, 1F | 59.4 ± 6.75 *ɛ | 30.4 ± 1.78 |
ALL | 5M, 3F | 34.4 ± 5.51 | 25.9 ± 2.38 |
CLL | 7M, 8F | 66.5 ± 3.42 *ɛ | 28.1 ± 1.98 |
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Alshaalan, K.S.; Albawardi, T.K.; Zhra, M.; Bin Sulaiman, N.; Jnied, O.Y.; Saleem, R.A.; Aljada, A. Differential Expression of LMNA/C and Insulin Receptor Transcript Variants in Peripheral Blood Mononuclear Cells of Leukemia Patients. J. Clin. Med. 2024, 13, 2568. https://doi.org/10.3390/jcm13092568
Alshaalan KS, Albawardi TK, Zhra M, Bin Sulaiman N, Jnied OY, Saleem RA, Aljada A. Differential Expression of LMNA/C and Insulin Receptor Transcript Variants in Peripheral Blood Mononuclear Cells of Leukemia Patients. Journal of Clinical Medicine. 2024; 13(9):2568. https://doi.org/10.3390/jcm13092568
Chicago/Turabian StyleAlshaalan, Khalid Saud, Turki Khalid Albawardi, Mahmoud Zhra, Norah Bin Sulaiman, Osama Yaheia Jnied, Rimah Abdullah Saleem, and Ahmad Aljada. 2024. "Differential Expression of LMNA/C and Insulin Receptor Transcript Variants in Peripheral Blood Mononuclear Cells of Leukemia Patients" Journal of Clinical Medicine 13, no. 9: 2568. https://doi.org/10.3390/jcm13092568