Expression and Functional Analysis of the Smo Protein in Apis mellifera
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Drug Feeding
2.3. Total RNA Extraction and cDNA Synthesis
2.4. Smo Gene Amplification
2.5. Expression of Smo
2.6. Localization of Smo Genes in the Antennae
2.7. EAG Recording
2.8. Behavioral Tests
2.9. Statistical Analysis
3. Results
3.1. Identification of Smo Genes
3.2. Analysis of Differential Tissue Expression of Smo
3.3. Location of Smo
3.4. Effects of Cyclopamine and Purmorphamine on Smo Gene Expression
3.4.1. Cyclopamine
3.4.2. Purmorphamine
3.4.3. Regulation of OR by Smo
3.5. EAG Recording
3.6. Y-Tube Olfactometer Experiments
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′-3′) | Purpose |
---|---|---|
P1 | F: TCATTATTCCTAAAATTTCCCGCGT | Sequence amplification |
F: CAAATCCCTGCAGCCCAAG | ||
R: TGAATGAACCGCAAATCCCTG | ||
P2 | F: ATTTCTAGCAAATGCTTAAAGCCTT | |
R: GCACCTCTACGAGTAACTAACTTTGGTA | ||
P3 | F: AAACATTTAATAATGCTGGTCGATT | |
F: TTAATAATGCTGGTCGATTATCTATTAGT | ||
R: TTTTATATTAAAAGTTTTTCGAAAAAGAA | ||
Smo | F: TGGTGTTTGCCACTTGTCCT | qRT-PCR |
R: ACCAAGCTCTGACTGCATGA | ||
Arp1 | F: TGCTGCACTCGTAGTTGACAATGG | |
R: ACCCTGGTGGCGTGGTCTTC | ||
OR151 | F: AAACACCGGAAAGAGGTATGG | |
R: TGCATCCAGCATACGAACAG | ||
OR152 | F: CGCTCTTTTGCTCTCTGGTTCG | |
R: TGATCAGCAGGCCAAGCATA | ||
OR2 | F: CTCGTGGGCTCCTGTTCGCTTG | |
R: CTGTTCCTTCGGGCTGCTCTGC |
Compounds | CAS Number | Purity (%) | Origin |
---|---|---|---|
Linalool | 78-70-6 | >98 | Macklin |
Myrcene | 123-35-3 | >90 | Macklin |
6-methyl-5-heptene-2-one (6-M) | 110-93-0 | >98 | Macklin |
neral | 5392-40-5 | >97 | Macklin |
2-((4-Ethyl-5-(pyridin-3-yl)-4H-1,2,4-triazol-3-yl) thio)-N-(4-ethylphenyl) acetamidea (VUAA1) | 525582-84-7 | >97.5 | Macklin |
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Guo, L.; Wang, J.; Yu, D.; Zhang, Y.; Zhang, H.; Guo, Y. Expression and Functional Analysis of the Smo Protein in Apis mellifera. Insects 2024, 15, 555. https://doi.org/10.3390/insects15070555
Guo L, Wang J, Yu D, Zhang Y, Zhang H, Guo Y. Expression and Functional Analysis of the Smo Protein in Apis mellifera. Insects. 2024; 15(7):555. https://doi.org/10.3390/insects15070555
Chicago/Turabian StyleGuo, Lina, Jue Wang, Diandian Yu, Yu Zhang, Huiman Zhang, and Yuan Guo. 2024. "Expression and Functional Analysis of the Smo Protein in Apis mellifera" Insects 15, no. 7: 555. https://doi.org/10.3390/insects15070555