Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells
Abstract
:1. Introduction
2. Results
2.1. Detection of Gene Locations with Differential H3K27ac Occupancy Following Fluoride Treatment
2.2. Comparison of Gene Expressions and H3K27ac Occupancy Following Fluoride Treatment
2.3. Fluoride-Induced p21 and Mdm2 mRNA Levels Are Suppressed by HAT Inhibitors (AA, MG149)
2.4. Fluoride Suppresses the Activity of Class I and II HDACs
2.5. Sodium Butyrate Suppresses the Activity of Class I, II, and III HDACs and Increases Histone Acetylation
2.6. Addition of SB Increases the mRNA Levels of Bcl2 and Bad Compared to Fluoride Treatment Alone
2.7. Addition of SB Suppresses Fluoride-Mediated Apoptosis by Decreasing the mRNA Levels of p21, Mdm2, and the Bax/Bcl2 Ratio
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. ChIP-Sequence
4.3. Real Time PCR
4.4. Western Blotting
4.5. Immunofluorescence
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Histone | Chromosome | PeakStart | PeakEnd | Distance | GeneStart | GeneEnd | ClosestTSS_ID | Symbol | Strand | Enrichment | Qvalues | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
acetylated | H3K27 | chr7 | 52,721,282 | 52,722,261 | 373 | 52,717,232 | 52,722,145 | ENSMUST00000169539 | Bax | − | 2.65055 | 4.98461 |
chr10 | 117,146,295 | 117,147,436 | 212 | 117,125,951 | 117,147,078 | ENSMUST00000155285 | Mdm2 | − | 2.47539 | 4.63473 | ||
chr17 | 29,230,713 | 29,231,971 | 625 | 29,230,717 | 29,237,667 | ENSMUST00000023829 | p21 | + | 2.31322 | 5.8589 | ||
De-acetylated | H3K27 | chr1 | 108,610,964 | 108,611,451 | −356 | 108,434,755 | 108,610,851 | ENSMUST00000112751 | Bcl2 | − | 2.49999 | 1.6118 |
chr1 | 108,610,216 | 108,610,628 | 429 | 108,434,755 | 108,610,851 | ENSMUST00000112751 | Bcl2 | − | 2.84654 | 1.61343 | ||
chr11 | 69,394,210 | 69,394,667 | 531 | 69,393,907 | 69,405,375 | ENSMUST00000108657 | Trp53 | + | 1.63639 | 2.34386 | ||
chr19 | 7,016,341 | 7,017,061 | −112 | 7,016,813 | 7,026,382 | ENSMUST00000113426 | Bad | + | 2.19519 | 3.62331 |
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Yamashita, S.; Okamoto, M.; Mendonca, M.; Fujiwara, N.; Kitamura, E.; Chang, C.-S.S.; Brueckner, S.; Shindo, S.; Kuriki, N.; Cooley, M.A.; et al. Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells. Int. J. Mol. Sci. 2024, 25, 9600. https://doi.org/10.3390/ijms25179600
Yamashita S, Okamoto M, Mendonca M, Fujiwara N, Kitamura E, Chang C-SS, Brueckner S, Shindo S, Kuriki N, Cooley MA, et al. Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells. International Journal of Molecular Sciences. 2024; 25(17):9600. https://doi.org/10.3390/ijms25179600
Chicago/Turabian StyleYamashita, Shohei, Motoki Okamoto, Melanie Mendonca, Natsumi Fujiwara, Eiko Kitamura, Chang-Sheng Sam Chang, Susanne Brueckner, Satoru Shindo, Nanako Kuriki, Marion A. Cooley, and et al. 2024. "Fluoride Alters Gene Expression via Histone H3K27 Acetylation in Ameloblast-like LS8 Cells" International Journal of Molecular Sciences 25, no. 17: 9600. https://doi.org/10.3390/ijms25179600