Transcription Factor VlbZIP14 Inhibits Postharvest Grape Berry Abscission by Directly Activating VlCOMT and Promoting Lignin Biosynthesis
Abstract
:1. Introduction
2. Results
2.1. Treatment with Chitosan and Eugenol Inhibits SO2-Induced Berry Abscission during Storage
2.2. Treatment with Chitosan and Eugenol Maintains the Quality of Table Grapes during Storage
2.3. Treatment with Chitosan and Eugenol Reduces the Lipid Peroxidation of Table Grapes during Storage
2.4. Treatment with Chitosan and Eugenol Inhibits the Activity of Cell Wall-Degrading Enzymes of Table Grapes
2.5. Transcriptome Analysis of SO2-Induced Abscission of Berries Treated with Eugenol-Loaded Chitosan Nanoparticles
2.6. SN Treatment-Related Modules Screened by WGCNA
2.7. COMT Promotes the Synthesis of Lignin and Inhibits Berry Abscission
2.8. VlCOMT Overexpression Promotes Lignin Synthesis
2.9. VlbZIP14 Specifically Activates the VlCOMT Promoter
3. Discussion
4. Methods
4.1. Plant Materials and Exogenous Treatment
4.2. Abscission Rate and Weight Loss
4.3. Anthocyanin and Vitamin C (VC) Content
4.4. Malondialdehyde (MDA) and Hydrogen Peroxide (H2O2) Content
4.5. Enzyme Activity Assays
4.6. Transcriptome Deep Sequencing (RNA-seq) Library Preparation and Sequencing
4.7. Analysis of DEGs
4.8. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Enrichment Analysis
4.9. Cluster Analysis and WGCNA
4.10. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.11. Kinetic Parameters of Catalytic Reaction
4.12. High-Performance Liquid Chromatography (HPLC) Assay
4.13. Determination of Lignin, Cellulose, and Hemicellulose Content
4.14. Generation of Transgenic Tomato Plants
4.15. Subcellular Localization of VlbZIP14
4.16. Dual Luciferase Assay
4.17. Yeast One-Hybrid (Y1H) Assay
4.18. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yu, P.; Li, S.; Sun, Y.; Meng, X.; Shi, Q.; Zhao, X.; Yu, Y. Transcription Factor VlbZIP14 Inhibits Postharvest Grape Berry Abscission by Directly Activating VlCOMT and Promoting Lignin Biosynthesis. Int. J. Mol. Sci. 2024, 25, 9479. https://doi.org/10.3390/ijms25179479
Yu P, Li S, Sun Y, Meng X, Shi Q, Zhao X, Yu Y. Transcription Factor VlbZIP14 Inhibits Postharvest Grape Berry Abscission by Directly Activating VlCOMT and Promoting Lignin Biosynthesis. International Journal of Molecular Sciences. 2024; 25(17):9479. https://doi.org/10.3390/ijms25179479
Chicago/Turabian StyleYu, Peng, Songqi Li, Yadan Sun, Xiangxuan Meng, Qiaofang Shi, Xiaochun Zhao, and Yihe Yu. 2024. "Transcription Factor VlbZIP14 Inhibits Postharvest Grape Berry Abscission by Directly Activating VlCOMT and Promoting Lignin Biosynthesis" International Journal of Molecular Sciences 25, no. 17: 9479. https://doi.org/10.3390/ijms25179479