Alterations of the Fatty Acid Profile and the Expression of Genes Related to FA Metabolism in Cirrhotic Liver Tissue
Abstract
:1. Introduction
2. Results
2.1. Effect of Cirrhosis on Hepatic FA Profile
2.2. Effect of Cirrhosis on Hepatic FA Metabolism
3. Discussion
4. Materials and Methods
4.1. Subjects
4.2. Sample Collection
4.3. Sample Preparation
4.4. Gas Chromatography–Mass Spectrometry Analysis
4.5. Analysis of mRNA Level
4.6. Analysis of Hydroxyproline Content
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Parameter | Hepatic Control | Patients with Cirrhosis | p Value |
---|---|---|---|
Age (years) | 57.9 ± 17.6 | 50.6 ± 11.3 | NS |
BMI (kg/m3) | 27.5 (23.5; 32.0) | 26.0 (23.5; 32.5) | NS |
MELD-Na | 7.00 ± 1.63 | 17.0 ± 7.27 | 0.012 * |
Total cholesterol (mg/dL) | 151 ± 38.1 | 138 ± 50.9 | NS |
LDL-C (mg/dL) | 80.9 ± 27.9 | 90.0 ± 40.3 | NS |
HDL-C (mg/dL) | 51.2 ± 15.2 | 35.0 ± 13.3 | 0.017 * |
TAG (mg/dL) | 98.0 ± 43.9 | 91.7 ± 24.7 | NS |
Glucose (mg/dL) | 96.0 (89.5; 110) | 101 (93.0; 116) | NS |
Bilirubin (mg/dL) | 0.58 (0.40; 0.75) | 2.0 (1.16; 4.05) | <0.001 ## |
GGTP (IU/L) | 43.0 (24.0; 96.0) | 79.0 (47.0; 238) | NS |
ALT (U/L) | 20.0 (17.0; 41.0) | 36.0 (13.0; 72.0) | NS |
AST (U/L) | 25.5 (19.0; 37.8) | 51.0 (28.5; 90.5) | 0.033 # |
WBC (109/l) | 7.37 (5.66; 8.87) | 5.46 (3.56; 7.54) | 0.022 # |
ALP (U/L) | 76.0 (66.5; 113) | 116 (91.3; 218) | 0.034 # |
Creatinine (mg/dL) | 0.81 (0.69; 0.94) | 0.82 (0.67; 1.25) | NS |
Albumin (g/L) | 38.6 ± 3.75 | 33.2 ± 6.81 | 0.028 * |
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Hliwa, A.; Lange-Andrzejewska, O.; Laski, D.; Sledzinski, M.; Remiszewski, P.; Drobinska, A.; Mika, A.; Sledzinski, T. Alterations of the Fatty Acid Profile and the Expression of Genes Related to FA Metabolism in Cirrhotic Liver Tissue. Int. J. Mol. Sci. 2024, 25, 8115. https://doi.org/10.3390/ijms25158115
Hliwa A, Lange-Andrzejewska O, Laski D, Sledzinski M, Remiszewski P, Drobinska A, Mika A, Sledzinski T. Alterations of the Fatty Acid Profile and the Expression of Genes Related to FA Metabolism in Cirrhotic Liver Tissue. International Journal of Molecular Sciences. 2024; 25(15):8115. https://doi.org/10.3390/ijms25158115
Chicago/Turabian StyleHliwa, Aleksandra, Oliwia Lange-Andrzejewska, Dariusz Laski, Maciej Sledzinski, Piotr Remiszewski, Anna Drobinska, Adriana Mika, and Tomasz Sledzinski. 2024. "Alterations of the Fatty Acid Profile and the Expression of Genes Related to FA Metabolism in Cirrhotic Liver Tissue" International Journal of Molecular Sciences 25, no. 15: 8115. https://doi.org/10.3390/ijms25158115