Addition of Polyphenols to Drugs: The Potential of Controlling “Inflammaging” and Fibrosis in Human Senescent Lung Fibroblasts In Vitro
Abstract
:1. Introduction
2. Results
2.1. Senomorphic Effects of Drugs and Polyphenols
2.2. Evaluation of the Antifibrotic Activity of Drugs and Polyphenols
2.3. EMT Evaluation
3. Discussion
4. Materials and Methods
4.1. MRC-5 Cell Culture
4.2. MRC-5 Senescence Induced by Doxorubicin
4.3. Determination of Drug and Polyphenol Non-Toxic Concentrations
4.4. SASP Evaluation
4.5. Senescence-Associated β-Galactosidase (SA-β-gal) Assay
4.6. Quantitative Analysis of Gene Expression
4.7. Picrosirius Red and Crystal Violet Stains
4.8. Epithelial-Mesenchymal Transition (EMT) Assay Using A549 Cells
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Godoy, M.C.X.d.; Monteiro, G.A.; Moraes, B.H.d.; Macedo, J.A.; Gonçalves, G.M.S.; Gambero, A. Addition of Polyphenols to Drugs: The Potential of Controlling “Inflammaging” and Fibrosis in Human Senescent Lung Fibroblasts In Vitro. Int. J. Mol. Sci. 2024, 25, 7163. https://doi.org/10.3390/ijms25137163
Godoy MCXd, Monteiro GA, Moraes BHd, Macedo JA, Gonçalves GMS, Gambero A. Addition of Polyphenols to Drugs: The Potential of Controlling “Inflammaging” and Fibrosis in Human Senescent Lung Fibroblasts In Vitro. International Journal of Molecular Sciences. 2024; 25(13):7163. https://doi.org/10.3390/ijms25137163
Chicago/Turabian StyleGodoy, Maria Carolina Ximenes de, Gabriela Arruda Monteiro, Bárbara Hakim de Moraes, Juliana Alves Macedo, Gisele Mara Silva Gonçalves, and Alessandra Gambero. 2024. "Addition of Polyphenols to Drugs: The Potential of Controlling “Inflammaging” and Fibrosis in Human Senescent Lung Fibroblasts In Vitro" International Journal of Molecular Sciences 25, no. 13: 7163. https://doi.org/10.3390/ijms25137163