Vx-809, a CFTR Corrector, Acts through a General Mechanism of Protein Folding and on the Inflammatory Process
Abstract
:1. Introduction
2. Results
2.1. Vx-809 Interferes with UPR Activation
2.2. Vx-809 Counteract Thapsigargin-Induced Apoptotic Response
2.3. Vx-809 Is Involved in Intracellular and Mitochondrial ROS Production
2.4. The “Corrector” Vx-809 Reduces Thapsigargin-Induced Ca2+ Homeostasis Dysregulation
2.5. Effect of Vx-809 on the Inflammatory Pathway
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. Experimental Protocol
4.4. Protein Extraction and Western Blot Analysis
4.5. Measurement of Intracellular Calcium Signaling
4.6. Intracellular and Mitochondrial ROS Release Measurement
4.7. Flow Cytometry Analysis
4.8. Determination of Hypodiploid DNA
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pecoraro, M.; Serra, A.; Pascale, M.; Franceschelli, S. Vx-809, a CFTR Corrector, Acts through a General Mechanism of Protein Folding and on the Inflammatory Process. Int. J. Mol. Sci. 2023, 24, 4252. https://doi.org/10.3390/ijms24044252
Pecoraro M, Serra A, Pascale M, Franceschelli S. Vx-809, a CFTR Corrector, Acts through a General Mechanism of Protein Folding and on the Inflammatory Process. International Journal of Molecular Sciences. 2023; 24(4):4252. https://doi.org/10.3390/ijms24044252
Chicago/Turabian StylePecoraro, Michela, Adele Serra, Maria Pascale, and Silvia Franceschelli. 2023. "Vx-809, a CFTR Corrector, Acts through a General Mechanism of Protein Folding and on the Inflammatory Process" International Journal of Molecular Sciences 24, no. 4: 4252. https://doi.org/10.3390/ijms24044252