IAA Plays an Important Role in Alkaline Stress Tolerance by Modulating Root Development and ROS Detoxifying Systems in Rice Plants
Abstract
:1. Introduction
2. Results
2.1. Dongdao-4 Seedlings Are More Tolerant to Alkaline Stress than Jigeng-88 Seedlings
2.2. Effect of Alkaline Stress on Auxin Concentration
2.3. Effect of IAA and TIBA on Plant Growth under Alkaline Stress
2.4. Effect of IAA and TIBA on Foliar Chlorophyll Concentration and Photosynthetic Rates under Alkaline Stress
2.5. Effect of IAA and TIBA on Root System Architecture under Alkaline Stress
2.6. Effect of IAA and TIBA on the Content of H2O2 and Malondialdehyde under Alkaline Stress
2.7. Effect of IAA and TIBA on Activities of Antioxidant Enzymes under Alkaline Stress
2.8. Effect of Alkaline Stress on the Genes Involved in IAA Biosynthesis, Transport and Catabolism
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Germination Treatments
4.2. Measurements of Plant Growth
4.3. Measurements of Chlorophyll (CHL) Concentration
4.4. Measurements of Photosynthetic Characteristics
4.5. Determination of H2O2
4.6. Determination of Malondialdehyde (MDA)
4.7. Determination of Peroxidase, Superoxide Dismutase, and Catalase Activity
4.8. RNA Isolation and Real-Time RT-PCR
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Ma, C.; Yuan, S.; Xie, B.; Li, Q.; Wang, Q.; Shao, M. IAA Plays an Important Role in Alkaline Stress Tolerance by Modulating Root Development and ROS Detoxifying Systems in Rice Plants. Int. J. Mol. Sci. 2022, 23, 14817. https://doi.org/10.3390/ijms232314817
Ma C, Yuan S, Xie B, Li Q, Wang Q, Shao M. IAA Plays an Important Role in Alkaline Stress Tolerance by Modulating Root Development and ROS Detoxifying Systems in Rice Plants. International Journal of Molecular Sciences. 2022; 23(23):14817. https://doi.org/10.3390/ijms232314817
Chicago/Turabian StyleMa, Changkun, Shuai Yuan, Biao Xie, Qian Li, Quanjiu Wang, and Mingan Shao. 2022. "IAA Plays an Important Role in Alkaline Stress Tolerance by Modulating Root Development and ROS Detoxifying Systems in Rice Plants" International Journal of Molecular Sciences 23, no. 23: 14817. https://doi.org/10.3390/ijms232314817