Generation and Characterization of a Zebrafish IL-2Rγc SCID Model
Abstract
:1. Introduction
2. Results
2.1. Generation of Il-2rγc.a Mutants
2.2. Impact on Embryonic Lymphopoiesis
2.3. Impact on Later Lymphopoiesis
2.4. Impact on Intestinal Microbiota
2.5. Impact on Anti-Tumor Immunity
3. Discussion
4. Materials and Methods
4.1. Zebrafish Husbandry and Manipulation
4.2. Reverse-Transcription Polymerase Chain Reaction
4.3. Whole-Mount in Situ Hybridization (WISH)
4.4. Ex Vivo Analysis
4.5. Tumor Xenotransplantation
4.6. Imaging
4.7. Statistical Analysis
4.8. Microbiome Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sertori, R.; Jones, R.; Basheer, F.; Rivera, L.; Dawson, S.; Loke, S.; Heidary, S.; Dhillon, A.; Liongue, C.; Ward, A.C. Generation and Characterization of a Zebrafish IL-2Rγc SCID Model. Int. J. Mol. Sci. 2022, 23, 2385. https://doi.org/10.3390/ijms23042385
Sertori R, Jones R, Basheer F, Rivera L, Dawson S, Loke S, Heidary S, Dhillon A, Liongue C, Ward AC. Generation and Characterization of a Zebrafish IL-2Rγc SCID Model. International Journal of Molecular Sciences. 2022; 23(4):2385. https://doi.org/10.3390/ijms23042385
Chicago/Turabian StyleSertori, Robert, Realla Jones, Faiza Basheer, Leni Rivera, Samantha Dawson, Stella Loke, Somayyeh Heidary, Amardeep Dhillon, Clifford Liongue, and Alister C. Ward. 2022. "Generation and Characterization of a Zebrafish IL-2Rγc SCID Model" International Journal of Molecular Sciences 23, no. 4: 2385. https://doi.org/10.3390/ijms23042385