Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene Nanoplastics
Abstract
:1. Introduction
2. Results
2.1. Uptake of PS Nanoplastics into MEFs via Endocytosis
2.2. Activation of the Stress Response System in MEFs Caused by the Intracellular Accumulation of PS Nanoplastics
2.3. Clearance of PS Nanoplastics from the Cytoplasm of MEFs
3. Discussion
4. Materials and Methods
4.1. Isolation and Culture of Mouse Embryonic Fibroblasts
4.2. Polystyrene Nanoplastic Treatment
4.3. Blocking Endocytosis
4.4. Reactive Oxygen Species Detection Assay
4.5. Quantitative Reverse Transcription PCR
4.6. Immunoblot Analysis
4.7. Immunofluorescence Analysis
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Han, S.-W.; Choi, J.; Ryu, K.-Y. Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene Nanoplastics. Int. J. Mol. Sci. 2021, 22, 2094. https://doi.org/10.3390/ijms22042094
Han S-W, Choi J, Ryu K-Y. Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene Nanoplastics. International Journal of Molecular Sciences. 2021; 22(4):2094. https://doi.org/10.3390/ijms22042094
Chicago/Turabian StyleHan, Seung-Woo, Jinhee Choi, and Kwon-Yul Ryu. 2021. "Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene Nanoplastics" International Journal of Molecular Sciences 22, no. 4: 2094. https://doi.org/10.3390/ijms22042094