Next Article in Journal
Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures
Next Article in Special Issue
The Potential Protective Role of RUNX1 in Nonalcoholic Fatty Liver Disease
Previous Article in Journal
Asciminib Mitigates DNA Damage Stress Signaling Induced by Cyclophosphamide in the Ovary
Previous Article in Special Issue
The Role of the Gut Microbiome in Liver Cirrhosis Treatment
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 3
10.3390/ijms22031397
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Bile Acids and Microbiota: Multifaceted and Versatile Regulators of the Liver–Gut Axis

by
Niklas Grüner
1 and
Jochen Mattner
1,2,*
1
Mikrobiologisches Institut-Klinische Mikrobiologie, Immunologie und Hygiene, Universitätsklinikum Erlangen and Friedrich-Alexander Universität (FAU) Erlangen-Nürnberg, 91054 Erlangen, Germany
2
Medical Immunology Campus Erlangen, FAU Erlangen-Nürnberg, 91054 Erlangen, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(3), 1397; https://doi.org/10.3390/ijms22031397
Submission received: 23 December 2020 / Revised: 25 January 2021 / Accepted: 28 January 2021 / Published: 30 January 2021
(This article belongs to the Special Issue Liver–Gut Axis)
Browse Figures
Review Reports Versions Notes

Abstract

:
After their synthesis from cholesterol in hepatic tissues, bile acids (BAs) are secreted into the intestinal lumen. Most BAs are subsequently re-absorbed in the terminal ileum and are transported back for recycling to the liver. Some of them, however, reach the colon and change their physicochemical properties upon modification by gut bacteria, and vice versa, BAs also shape the composition and function of the intestinal microbiota. This mutual interplay of both BAs and gut microbiota regulates many physiological processes, including the lipid, carbohydrate and energy metabolism of the host. Emerging evidence also implies an important role of this enterohepatic BA circuit in shaping mucosal colonization resistance as well as local and distant immune responses, tissue physiology and carcinogenesis. Subsequently, disrupted interactions of gut bacteria and BAs are associated with many disorders as diverse as Clostridioides difficile or Salmonella Typhimurium infection, inflammatory bowel disease, type 1 diabetes, asthma, metabolic syndrome, obesity, Parkinson’s disease, schizophrenia and epilepsy. As we cannot address all of these interesting underlying pathophysiologic mechanisms here, we summarize the current knowledge about the physiologic and pathogenic interplay of local site microbiota and the enterohepatic BA metabolism using a few selected examples of liver and gut diseases.

1. Introduction

The microenvironment, the tissue milieu and metabolic processes of the host’s own cells and colonizing microbial commensals have a tremendous impact on the development and function of various hematopoietic and non-hematopoietic cell populations in humans. The gut microbiota and their metabolites, for example, contribute to the regulation of many physiological processes and exhibit a pivotal role in maintaining human health. Thus, microbiota signal not only to neighboring but also to distant organs and tissues in the body and interfere, for example, with the brain (brain–gut axis), the liver (liver–gut axis) and the immune and hormone system of the host [1], and vice versa, the host also shapes the intraluminal microbiota. Although the genetic traits of the host as well as dietary and micro-environmental factors presumably contribute to these complex interactions [2,3,4,5,6], it is largely unknown how commensal microbiota are selectively influenced.
Nonetheless, disrupted intestinal microbiota that accompany inflammatory processes in the gut and the subsequent altered availability of microbial- or host-derived products and metabolites are frequently associated with the pathogenesis of various complex disorders [7,8,9,10]. As the etiology of these diseases is multifactorial, they develop as a consequence of a malfunctioning network rather than of a single cause [11]. Thus, the versatile host–microbiota interactions are pivotal for maintaining physiologic homeostasis.
One interesting aspect of host–microbiota interactions is that host- and microbiota-derived enzymes frequently regulate the same pool of intraluminal metabolites [12]. Some physiologic signaling cascades and metabolic processes even require co-metabolism between the host and the microbiota. A classic example of shared alternating enzymatic reactions between mammalians and their intraluminal microbiota is the biosynthesis of bile acids (BAs). In this case, the host synthesizes primary BAs in the liver, which the intestinal microbiota subsequently transform in the gut. This conjugation and deconjugation of BAs plays a pivotal role in many physiological and pathophysiological processes in the liver–gut axis. Here, we discuss a few of them using selected examples of infectious, immune-mediated and tumor diseases.

2. Bile Acids (BAs) and Enterohepatic Circulation

Bile acids (BAs) are hydroxylated, amphipathic steroid acids that are synthesized in the peroxisomes of the liver from cholesterol [13]. In hepatic tissues, BAs are also conjugated to the hydrophilic amino acids glycine (Gly), taurine (Tau) or sulfate. These BAs are now named primary or conjugated BAs. They consist of cholic acid (CA) and chenodeoxycholic acid (CDCA) or tauro- and glycoconjugated versions thereof (Table 1). While humans preferably use glycine for conjugation, taurine is the most common conjugate of BAs in rodents.
Once synthesized, these primary BAs are secreted into the bile, where they comprise about 80% of the organic compounds [29] and are concentrated for storage in the gallbladder [30]. Ingestion of food triggers the release of cholecystokinin by enteroendocrine cells, which causes gallbladder contraction and the release of primary BAs into the duodenum [31]. There, the primary purpose of BAs is to facilitate the digestion and absorption of dietary lipids, fatty acids, cholesterol, fat-soluble vitamins and other hydrophobic components of the diet via its surfactant properties, which emulsify fats into micelles [14]. Usually, more than 95% of the primary BAs are re-absorbed from the terminal ileum and transported back into the liver via the so-called enterohepatic circulation (EHC) (Figure 1). Upon their return to the liver via the portal vein, primary BAs inhibit cholesterol biosynthesis and further BA biosynthesis [29].
However, small quantities of primary BAs also reach the colon, where certain gut bacteria transform them into secondary BAs, such as deoxycholic acid (DCA), ursodeoxycholic (UDCA) and lithocholic acid (LCA), by deconjugation, oxidation/epimerization, (7-α-) dehydroxylation and esterification [32,33,34,35] (Table 2). In particular, secondary BAs exhibit strong antimicrobial activity and cytotoxicity. Thus, BAs also regulate the composition of gut bacterial communities and host physiology [32], features that are described in more detail below. Immunoglobulin A (IgA), the major immunoglobulin at mucosal surfaces, enhances the antimicrobial properties of BAs; thus, both BAs and IgA inhibit bacterial growth and adhesion and subsequently protect against ascending infections within the biliary tract. In addition, BAs eliminate bilirubin from the body via the feces [29,36].
Both, primary and secondary BAs exert biological effects on cells of the host by activating nuclear and plasma membrane receptors, including the nuclear farnesoid X receptor (FXR) or the G protein-coupled receptor (TGR5) [37,38,39,40]. These receptors control the synthesis and metabolism of BAs. However, engagement of these receptors enables BAs to contribute to the regulation of glucose homeostasis, lipid metabolism and energy expenditure [50]. Furthermore, BAs regulate immune responses upon ligation of these two receptors (Figure 2), which are located at the interface of the host immune system with the intestinal microbiota [51]. Both receptors are highly expressed on cells of the innate immune system, including macrophages, dendritic cells and natural killer T (NKT) cells [51]. In particular, TGR5 and FXR regulate the polarization of macrophages and can also rescue mice from severe colitis [52,53].
In summary, the main functions of BAs include (a) emulsifying and digesting fat, (b) regulating and excreting cholesterol, (c) exerting antimicrobial effects and (d) eliminating bilirubin from the body [29,36]. However, as there exist multiple microbes that are tolerant against bile [54], the antimicrobial effects of bile (acids) selectively restrain certain microbial species and subsequently affect the composition of the complete intestinal or biliary microflora.

3. Local Site Microbiota in the Gut and Bile

The intestine hosts complex and dynamic populations of highly diverse microorganisms. These include various bacteria, archaea and eukarya, which form a mutually beneficial relationship with the host [4,7]. Diet, for example, strongly influences the composition of the microbiota [55,56], and vice versa, intestinal microbiota produce metabolites and extract nutrients from a large range of molecules that enzymes of the host are unable to convert [34]. Many of these nutrients and metabolites derived from commensal microbiota have been implicated in the development, homeostasis and function of the immune system, suggesting that microbial commensals influence host immunity via nutrient- and metabolite-dependent mechanisms [57]. Accordingly, an altered composition of the gut microbiota, known as dysbiosis, accompanies many intestinal and extra-intestinal disorders [1,58].
Similar to the gut, the gallbladder also harbors a complex microbiota. In contrast to the intestine, the microbiota of the biliary tract contains relatively low levels of Bacteroidetes, while numbers of Proteobacteria, Tenericutes, Actinobacteria and Cyanobacteria are increased [59,60,61]. Similar to what is observed with intestinal microbiota, although less well studied, the bile microbiota can be disrupted [59].

4. Crosstalk between BAs and Microbiota

The regulation of the BA pool is one example of the interference of the microbial metabolism with the host [62]. The deconjugation, oxidation/epimerization, (7-α-) dehydroxylation and esterification of BAs by the intestinal microbiota can dramatically change their physicochemical properties and subsequently affect their microbial toxicity and intestinal absorption.
For example, the deconjugation of BAs by microbial bile salt hydrolases (BSH)—abundant enzymes found in all major bacterial phyla [33]—enhances their intestinal re-absorption. Furthermore, it promotes the colonization of the gut by microbiota and can serve as a nutritional source of sulfur, nitrogen and carbon [63,64,65].
7-α-Dehydroxylation converts the primary BAs cholic (CA) and chenodeoxycholic acids (CDCA) into the secondary BAs deoxycholic (DCA) and lithocholic acids (LCA) [35]. It is quantitatively the most important and the most physiologically significant conversion of BAs in humans. Deoxycholic acid may even account for up to 25% of the total BA pool. The bacterial species that possess 7-α-dehydroxylation activity include members of the Firmicutes phylum, such as Clostridium or Eubacterium.
The effects of the intestinal microbiota presumably extend further beyond BA composition and biotransformation. In fact, germ-free and antibiotic-treated mice exhibit reduced BA excretion in the feces. In contrast, the BA pool is increased along with enhanced BA secretion and re-absorption from the intestine and an overall altered metabolic homeostasis of the host [66,67,68,69].
As mentioned above, their amphipathic character also supplies BAs with antimicrobial activities. Thus, BAs alter the fluidity, permeability and function of cellular membranes and membrane-bound proteins [54,70,71]. BAs also cause DNA damage and oxidative stress and affect the formation of RNAs and proteins [54,70,71,72]. The application of BAs expands Firmicutes at the expense of Bacteroidetes in the gut. Accordingly, increased intraluminal BA concentrations favor the growth of bacterial species that 7-α-dehydroxylate primary BAs into secondary BAs [73,74]. In contrast, lower intraluminal BA levels predominantly favor the growth of Gram-negative bacteria. In addition, BAs also influence the integrity of intestinal epithelial cells and mucosal immune responses and thus indirectly regulate the composition of microbial communities [68,75,76,77]. As discussed below, secondary BAs can also exhibit toxic effects (Table 2) and promote infectious, inflammatory or malignant diseases.

5. Functional Consequences

A disruption of the versatile interactions between BAs and microbiota can lead to many disorders. However, it has remained unclear whether compositional changes in the intestinal microbiota and/or the intraluminal metabolome are the consequence or the cause of the respective individual diseases. Nonetheless, dysbiosis usually accompanies many disorders, such as inflammatory bowel disease (IBD), or forms the basis for infections with gastrointestinal pathogens. Below, we summarize a few examples of these diseases affecting the liver–gut axis.

5.1. Infectious Disease

Microbial metabolism affects BA composition. Microbiota and microbiota-derived products also confer colonization resistance against many gastrointestinal pathogens, and vice versa, BAs as well as dietary habits shape the composition of the microbiota [78,79,80]. The loss of secondary BAs, for example, has been associated with susceptibility to infection by pathogenic bacteria. Conversely, a restoration of the secondary BA pool again promotes colonization resistance [26].
Dietary fat, for example, boosts intraluminal primary BA concentrations and subsequently gut colonization by Salmonella enteritidis serovar Typhimurium (S. Typhimurium) [16], as Salmonella spp. adapt to bile [17], upregulate the expression of virulence genes [18] and thus exhibit a much higher bile resistance than intestinal microbiota [16]. Survival strategies utilized by Salmonella serovars include the upregulation of efflux pumps and outer membrane proteins, the modification of lipopolysaccharide (LPS) and membrane structures and the induction of many virulence factors, including the repression of the type 3-secretion system (T3SS), which is essential for bacterial invasion of intestinal epithelial cells [20]. This ability of Salmonella spp. to survive in bile is a longstanding observation that is utilized for their selection and isolation in selective media, such as the bile-salt containing MacConkey agar [81].
Both commensal and pathogenic Escherichia coli (E. coli) strains utilize several mechanisms to resist bile. These include the activation of stress response genes, promoting the repair of DNA or membrane damage, the induction of efflux pumps or the upregulation of toxin/antitoxin systems to remove bile compounds [19,20,21,22,23]. Pathogenic E. coli strains, including enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC), also express virulence genes in response to bile exposure [20]. These primarily facilitate the colonization of the gut, the acquisition of nutrients and adhesion to host cells. However, further studies should explore whether there exists an altered resistance to detergents among different E. coli strains following bile exposure.
Exposure to bile in the small intestine also increases the virulence of Shigella flexneri and Shigella dysenteriae, two of the causative agents of Shigellosis [20], prior to reaching the site of infection in the colon. This manifests in enhanced protein secretion as well as an improved adherence to and invasion of intestinal epithelial cells.
Bile and the microbiota also influence the life cycle of Clostridioides difficile (C. difficile), a spore-forming Gram-positive bacterium and the causative agent of antibiotic-associated diarrhea (AAD). The application of antibiotics disrupts the commensal microbiota that converts primary BAs into secondary BAs, which usually inhibit the germination of C. difficile spores into vegetative bacteria [24,25,26]. Subsequently, the accumulation of primary BAs promotes the germination of C. difficile spores, bacterial replication and the production of colitis-mediating enterotoxins.
One of the few intestinal bacterial species that can actually convert primary BAs such as cholic acid into toxic secondary BAs such as deoxycholic acid is Clostridium (C.) scindens [82]. Due to the synthesis of C. difficile-inhibiting metabolites from host-derived bile salts, C. scindens enhances resistance to infection with C. difficile in a secondary BA-dependent fashion [83]. Thus, this BA-α-dehydroxylating member of the genus Clostridium is an example of a bile-mediated colonization resistance mechanism by intestinal microbiota. The knowledge of such mechanisms and the ecological context of microbes underlying these effects will facilitate the amplification of microbiota-mediated pathogen resistance in individuals at risk for infection.
Accumulating evidence demonstrates that intestinal microbiota also modulate enteric virus infections. For example, the modification of BAs by commensal bacteria primes type III interferon responses and subsequently inhibits an infection of the proximal small intestine with norovirus [84]. Primary and secondary BAs and their derivatives, such as glyco-ursodeoxycholic acid, and semi-synthetic derivatives, such as obeticholic acid, as well as ursodeoxycholic acid (UCDA), have been even recently suggested as therapy for SARS-CoV-2 infection due to the inhibition of virus binding to host cells or the suppression of the COVID-19-associated cytokine storm [85,86].

5.2. Inflammatory Bowel Disease (IBD)

Inflammatory bowel diseases (IBDs), such as ulcerative colitis (UC) and Crohn’s disease (CD), are characterized by immune-mediated inflammation within the gastrointestinal (GI) tract of affected patients [87]. Although the exact etiologies underlying UC and CD are unknown, both disorders are generally thought to result from a complex interplay of microbial, genetic, geographic and habitual factors [88,89], resulting in a dys-balanced interaction of symbiotic microorganisms, the intestinal epithelium and the immune system [90].
The absorption of BAs in the intestine is predominantly impaired in both pre-clinical models of experimental enterocolitis and in human IBD patients [27]. In addition, fecal BA composition remains altered in IBD patients who do not sustain remission [91]. Furthermore, the expression of the apical sodium-dependent bile acid transporter (ASBT), the major transporter for the efficient uptake of BAs in the terminal ileum [92], is suppressed in various animal models [27,93,94,95]. Accordingly, particularly CD patients exhibit reduced ASBT expression [96,97]. The subsequent increase of the intraluminal BA pool frequently manifests in the form of diarrhea due to a spillover of BAs into the colon [27]. Importantly, the restoration of the intestinal BA pool not only ameliorates clinical symptoms but also increases the numbers of regulatory T cells expressing the transcription factor RORγ and ameliorates host susceptibility to inflammatory colitis [98]. Increased levels of secondary BAs have been also associated with remission in UC patients following fecal microbiota transplantation (FMT) [99]. Thus, in summary, secondary BAs are associated with remission and improved clinical outcome in IBD, presumably reflecting a richer and more diverse microbiota.

5.3. Immune-Mediated Disease of the Liver

Chronic cholestatic liver diseases, such as primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC), are characterized by hepatic portal inflammation and slowly progress to obliterative fibrosis and eventually liver cirrhosis [100]. The subsequent obstruction of the bile flow alters the commensal microbiota of the gut and the biliary tract, susceptibility to infection and the integrity of the epithelial layers [61,101,102,103,104,105,106]. Indeed, increased concentrations of the pro-inflammatory and potentially cancerogenic agent taurolithocholic acid accompany biliary dysbiosis in PSC patients [101]. Furthermore, patients suffering from both PSC and IBD [107] exhibit distinct microbiota and microbiota-stool BA correlations as compared with IBD patients without concomitant PSC [108]. For example, the relative abundance of Clostridiales II and the overall bacterial diversity are lower in PSC patients compared with UC patients (without concomitant PSC) and control individuals [109]. Thus, microbial modifications of BAs likely modify the metabolism of the host, which can lead to altered immune signaling via BA receptors and modified immune responses. As microbial triggers play a role in the pathogenesis of both disorders [110,111], the altered BA composition in PBC and PSC might even allow unusual bacteria to expand and/or even perpetuate ascending infections within the biliary tree. Thus, there exists compelling evidence that microbial agents and/or changes in the intestinal/biliary microbiota, as well as an altered metabolite profile, including BAs, are involved in the pathogenesis of PBC and PSC. As pathogenic bacteria might use the biliary tree as a route for infection, and as ursodeoxycholic acid (UCDA, one of the secondary bile acids) is the only FDA-approved drug currently available for the treatment of PBC, its mechanism of action might include antimicrobial effects, as recently shown for C. difficile infections [112]. Whether the application of antibiotics might be a novel and alternative therapeutic option for the treatment of these devastating diseases needs to be addressed in future studies.

5.4. Hepato-Intestinal Carcinogenesis

It is well-known that the intestinal microbiota influences the efficacy of immunotherapy against tumors [113,114,115,116,117]. Furthermore, there exists increasing evidence that BAs and the intestinal microbiota promote intestinal and hepatic carcinogenesis [118,119,120]. Frequently, members of the genus Clostridiales are involved in these processes, presumably due to their capacity to convert primary into secondary BAs through 7-α-dehydroxylation [82].
Cholecystectomy alters the bile flow into the intestine and the enterohepatic circulation of BAs and can cause intestinal microbial dysbiosis [121,122,123]. The subsequent dysfunction of BA metabolism presumably positions patients at an increased risk for colorectal cancer (CRC). Thus, a lack of distinct intestinal microbiota promoted an accumulation of secondary BAs in the intestinal lumen, which may be a leading cause for the incidence of CRC in cholecystectomized patients [121,122,123]. The subsequent absence of surfactant protein D, which is synthesized in the gallbladder, contributes to the manifestation of intestinal dysbiosis [124].
Secondary BA-induced dysbiosis due to other medical reasons also promoted intestinal carcinogenesis [125] and adenomatous polyps [126], the most common precursor to CRC. In this context, primary BAs significantly inhibit cell responses following exposure to Bacteroides fragilis toxin (BFT or fragilysin), a metalloprotease encoded by enterotoxigenic Bacteroides fragilis (ETBF) that leads to tumorigenesis in susceptible mice, and is enriched in the mucosa of CRC patients [15] (Figure 3).
Clostridium butyricum, a butyrate-producing probiotic, inhibits intestinal tumor development by modulating Wnt signaling and gut microbiota [127]. Butyrate inhibited deoxycholic-acid-resistant colonic cell proliferation via cell cycle arrest and apoptosis [128]. The expression of the farnesoid X receptor (FXR), for which BAs are endogenous ligands [37,38,39,40], also influenced the Wnt/β-catenin signaling pathway and correlated inversely with the CRC stage and the clinical outcome [129]. Similarly, systemic butyrate also limited the antitumor effects of checkpoint inhibition [130]. Whether this was due to altered BA metabolism is currently unknown.
Removal of Clostridium species due to the application of antibiotics such as vancomycin promoted an accumulation of the primary bile acid chenodeoxycholic acid (CDCA). CDCA triggered the production of the chemokine CXCL16 by liver sinusoidal endothelial cells, which subsequently led to an accumulation of natural killer T (NKT) cells, an innate (-like) lymphocyte population endowed with potent immunomodulatory properties [131], in the liver and improved antitumor surveillance [28] (Figure 4).
Accordingly, alterations in the composition of intestinal microbiota involved in bile acid metabolism have been linked to the progression of hepatocellular carcinoma in mice and in patients with nonalcoholic steatohepatitis (NASH) [132,133]. Sex-dependent differences in BA profiles and altered hepatic BA retention in response to cholestyramine, a BA sequestrant, might thereby contribute to the sex-based disparity in liver carcinogenesis [134,135]. Furthermore, the deletion of thymine DNA glycosylase (TDG), a base excision repair enzyme that plays an essential role in the maintenance of epigenetic stability in cells [136], 8 weeks post-partum promoted hepatocellular carcinoma and dysregulation of BA homeostasis [119]. Future studies should investigate whether BA profiles and/or dysbiosis also affect pancreatic tumors.

6. Conclusions

There exists compelling evidence that co-metabolism between the host and the microbiota, as exemplified here for the enterohepatic circulation of BAs, is involved in the pathogenesis of many disorders of the gut–liver axis. Thus, the application of antibiotics to selectively deplete distinct microbiota or of BA sequestrants might be an alternative therapeutic option for the treatment of these complex disorders. However, many antibiotics are not specific for distinct bacterial species [137,138], similar to BA sequestrants for BAs. Thus, the choice of the substance, the duration of its use, its bile-permeability and its availability need to be carefully evaluated, especially with respect to the fact that many of the microbial species and their function for the maintenance of immune tolerance have not yet been elucidated. In order to address these pending questions, clinical studies must be initiated. Thus, therapeutic regimens need to be carefully considered in order to identify therapeutic targets and approaches that can be used to guide the development of effective therapies for dysbiosis and the disruption of BA metabolism, but also allow for the identification of common targets in enterohepatic diseases for clinical intervention in the future.

Author Contributions

N.G. collected the literature and prepared the figures. J.M. wrote the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the German Research Foundation (DFG), grants MA 2621/4-1 and CRC1181-project C04.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

We adhere to the Code of Conduct of the Committee on Publication Ethics (COPE) and to its Best Practice Guidelines.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

AADantibiotic-associated diarrhea
ASBTapical sodium-dependent bile acid transporter
BAbile acid
BFTBacteroides fragilis toxin
BSHbile salt hydrolases
CAcholic acid
CDCrohn’s disease
CDCAchenodeoxycholic acid
CRCcolorectal cancer
DCAdeoxycholic acid
EHCenterohepatic circulation
ETBFenterotoxigenic Bacteroides fragilis
FXRnuclear farnesoid X receptor
GIgastrointestinal tract
Glyglycine
HCChepatocellular carcinoma
IBDinflammatory bowel disease
IFNginterferon gamma
IgAimmunoglobulin A
IL1-binterleukin-1 beta
IL-6interleukin-6
IL-10interleukin-10
LCAlithocholic acid
LPSlipopolysaccharide
NASHnonalcoholic steatohepatitis
NKTnatural killer T cells
PBCprimary biliary cirrhosis
PSCprimary sclerosing cholangitis
Tautaurine
TDGthymine DNA glycosylase
TGF-btransforming growth factor beta
TNF-atumor necrosis factor alpha
T3SStype-3-secretion system
UCulcerative colitis
UCDAursodeoxycholic acid

References

  1. Schroeder, B.O.; Backhed, F. Signals from the gut microbiota to distant organs in physiology and disease. Nat. Med. 2016, 22, 1079–1089. [Google Scholar] [CrossRef] [PubMed]
  2. Liu, S.; da Cunha, A.P.; Rezende, R.M.; Cialic, R.; Wei, Z.; Bry, L.; Comstock, L.E.; Gandhi, R.; Weiner, H.L. The Host Shapes the Gut Microbiota via Fecal MicroRNA. Cell Host Microbe 2016, 19, 32–43. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Turnbaugh, P.J.; Hamady, M.; Yatsunenko, T.; Cantarel, B.L.; Duncan, A.; Ley, R.E.; Sogin, M.L.; Jones, W.J.; Roe, B.A.; Affourtit, J.P.; et al. A core gut microbiome in obese and lean twins. Nature 2009, 457, 480–484. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Backhed, F.; Ley, R.E.; Sonnenburg, J.L.; Peterson, D.A.; Gordon, J.I. Host-bacterial mutualism in the human intestine. Science 2005, 307, 1915–1920. [Google Scholar] [CrossRef] [Green Version]
  5. Goodrich, J.K.; Waters, J.L.; Poole, A.C.; Sutter, J.L.; Koren, O.; Blekhman, R.; Beaumont, M.; Van Treuren, W.; Knight, R.; Bell, J.T.; et al. Human genetics shape the gut microbiome. Cell 2014, 159, 789–799. [Google Scholar] [CrossRef] [Green Version]
  6. Ley, R.E.; Backhed, F.; Turnbaugh, P.; Lozupone, C.A.; Knight, R.D.; Gordon, J.I. Obesity alters gut microbial ecology. Proc. Natl. Acad. Sci. USA 2005, 102, 11070–11075. [Google Scholar] [CrossRef] [Green Version]
  7. Belkaid, Y.; Harrison, O.J. Homeostatic Immunity and the Microbiota. Immunity 2017, 46, 562–576. [Google Scholar] [CrossRef] [Green Version]
  8. Pickard, J.M.; Zeng, M.Y.; Caruso, R.; Nunez, G. Gut microbiota: Role in pathogen colonization, immune responses, and inflammatory disease. Immunol. Rev. 2017, 279, 70–89. [Google Scholar] [CrossRef]
  9. Xavier, J.B.; Young, V.B.; Skufca, J.; Ginty, F.; Testerman, T.; Pearson, A.T.; Macklin, P.; Mitchell, A.; Shmulevich, I.; Xie, L.; et al. The Cancer Microbiome: Distinguishing Direct and Indirect Effects Requires a Systemic View. Trends Cancer 2020, 6, 192–204. [Google Scholar] [CrossRef] [Green Version]
  10. Sharon, G.; Sampson, T.R.; Geschwind, D.H.; Mazmanian, S.K. The Central Nervous System and the Gut Microbiome. Cell 2016, 167, 915–932. [Google Scholar] [CrossRef] [Green Version]
  11. de Souza, H.S.P.; Fiocchi, C.; Iliopoulos, D. The IBD interactome: An integrated view of aetiology, pathogenesis and therapy. Nat. Rev. Gastroenterol. Hepatol. 2017, 14, 739–749. [Google Scholar] [CrossRef] [PubMed]
  12. Baier, J.; Gansbauer, M.; Giessler, C.; Arnold, H.; Muske, M.; Schleicher, U.; Lukassen, S.; Ekici, A.; Rauh, M.; Daniel, C.; et al. Arginase impedes the resolution of colitis by altering the microbiome and metabolome. J. Clin. Investig. 2020, 130, 5703–5720. [Google Scholar] [CrossRef] [PubMed]
  13. Ferdinandusse, S.; Houten, S.M. Peroxisomes and bile acid biosynthesis. Biochim. Biophys. Acta 2006, 1763, 1427–1440. [Google Scholar] [CrossRef] [Green Version]
  14. Hofmann, A.F.; Borgstroem, B. The Intraluminal Phase of Fat Digestion in Man: The Lipid Content of the Micellar and Oil Phases of Intestinal Content Obtained during Fat Digestion and Absorption. J. Clin. Investig. 1964, 43, 247–257. [Google Scholar] [CrossRef] [PubMed]
  15. Metz, P.; Tjan, M.J.H.; Wu, S.; Pervaiz, M.; Hermans, S.; Shettigar, A.; Sears, C.L.; Ritschel, T.; Dutilh, B.E.; Boleij, A. Drug Discovery and Repurposing Inhibits a Major Gut Pathogen-Derived Oncogenic Toxin. Front. Cell Infect. Microbiol. 2019, 9, 364. [Google Scholar] [CrossRef]
  16. Wotzka, S.Y.; Kreuzer, M.; Maier, L.; Arnoldini, M.; Nguyen, B.D.; Brachmann, A.O.; Berthold, D.L.; Zund, M.; Hausmann, A.; Bakkeren, E.; et al. Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice. Nat. Microbiol. 2019, 4, 2164–2174. [Google Scholar] [CrossRef]
  17. Hernandez, S.B.; Cota, I.; Ducret, A.; Aussel, L.; Casadesus, J. Adaptation and preadaptation of Salmonella enterica to Bile. PLoS Genet. 2012, 8, e1002459. [Google Scholar] [CrossRef] [Green Version]
  18. Johnson, R.; Ravenhall, M.; Pickard, D.; Dougan, G.; Byrne, A.; Frankel, G. Comparison of Salmonella enterica Serovars Typhi and Typhimurium Reveals Typhoidal Serovar-Specific Responses to Bile. Infect. Immun. 2018, 86, e00490-17. [Google Scholar] [CrossRef] [Green Version]
  19. Rosenberg, E.Y.; Bertenthal, D.; Nilles, M.L.; Bertrand, K.P.; Nikaido, H. Bile salts and fatty acids induce the expression of Escherichia coli AcrAB multidrug efflux pump through their interaction with Rob regulatory protein. Mol. Microbiol. 2003, 48, 1609–1619. [Google Scholar] [CrossRef]
  20. Sistrunk, J.R.; Nickerson, K.P.; Chanin, R.B.; Rasko, D.A.; Faherty, C.S. Survival of the Fittest: How Bacterial Pathogens Utilize Bile To Enhance Infection. Clin. Microbiol. Rev. 2016, 29, 819–836. [Google Scholar] [CrossRef] [Green Version]
  21. Kramer, V.C.; Nickerson, K.W.; Hamlett, N.V.; O’Hara, C. Prevalence of extreme detergent resistance among the Enterobacteriaceae. Can. J. Microbiol. 1984, 30, 711–713. [Google Scholar] [CrossRef] [PubMed]
  22. Kwan, B.W.; Lord, D.M.; Peti, W.; Page, R.; Benedik, M.J.; Wood, T.K. The MqsR/MqsA toxin/antitoxin system protects Escherichia coli during bile acid stress. Environ. Microbiol. 2015, 17, 3168–3181. [Google Scholar] [CrossRef] [PubMed]
  23. Sengupta, C.; Ray, S.; Chowdhury, R. Fine tuning of virulence regulatory pathways in enteric bacteria in response to varying bile and oxygen concentrations in the gastrointestinal tract. Gut. Pathog. 2014, 6, 38. [Google Scholar] [CrossRef] [PubMed]
  24. Giel, J.L.; Sorg, J.A.; Sonenshein, A.L.; Zhu, J. Metabolism of bile salts in mice influences spore germination in Clostridium difficile. PLoS ONE 2010, 5, e8740. [Google Scholar] [CrossRef] [Green Version]
  25. Sorg, J.A.; Sonenshein, A.L. Inhibiting the initiation of Clostridium difficile spore germination using analogs of chenodeoxycholic acid, a bile acid. J. Bacteriol. 2010, 192, 4983–4990. [Google Scholar] [CrossRef] [Green Version]
  26. Weingarden, A.R.; Chen, C.; Bobr, A.; Yao, D.; Lu, Y.; Nelson, V.M.; Sadowsky, M.J.; Khoruts, A. Microbiota transplantation restores normal fecal bile acid composition in recurrent Clostridium difficile infection. Am. J. Physiol. Gastrointest. Liver Physiol. 2014, 306, G310–G319. [Google Scholar] [CrossRef] [Green Version]
  27. Fitzpatrick, L.R.; Jenabzadeh, P. IBD and Bile Acid Absorption: Focus on Pre-clinical and Clinical Observations. Front. Physiol. 2020, 11, 564. [Google Scholar] [CrossRef]
  28. Ma, C.; Han, M.; Heinrich, B.; Fu, Q.; Zhang, Q.; Sandhu, M.; Agdashian, D.; Terabe, M.; Berzofsky, J.A.; Fako, V.; et al. Gut microbiome-mediated bile acid metabolism regulates liver cancer via NKT cells. Science 2018, 360. [Google Scholar] [CrossRef] [Green Version]
  29. Hofmann, A.F. The continuing importance of bile acids in liver and intestinal disease. Arch. Intern. Med. 1999, 159, 2647–2658. [Google Scholar] [CrossRef]
  30. Chen, I.; Cassaro, S. Physiology, Bile Acids; StatPearls: Treasure Island, FL, USA, 2020. [Google Scholar]
  31. Di Ciaula, A.; Garruti, G.; Lunardi Baccetto, R.; Molina-Molina, E.; Bonfrate, L.; Wang, D.Q.; Portincasa, P. Bile Acid Physiology. Ann. Hepatol. 2017, 16, s4–s14. [Google Scholar] [CrossRef]
  32. Ridlon, J.M.; Harris, S.C.; Bhowmik, S.; Kang, D.J.; Hylemon, P.B. Consequences of bile salt biotransformations by intestinal bacteria. Gut Microbes 2016, 7, 22–39. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  33. Jones, B.V.; Begley, M.; Hill, C.; Gahan, C.G.; Marchesi, J.R. Functional and comparative metagenomic analysis of bile salt hydrolase activity in the human gut microbiome. Proc. Natl. Acad. Sci. USA 2008, 105, 13580–13585. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. Gerard, P. Metabolism of cholesterol and bile acids by the gut microbiota. Pathogens 2013, 3, 14–24. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  35. Hamilton, J.P.; Xie, G.; Raufman, J.P.; Hogan, S.; Griffin, T.L.; Packard, C.A.; Chatfield, D.A.; Hagey, L.R.; Steinbach, J.H.; Hofmann, A.F. Human cecal bile acids: Concentration and spectrum. Am. J. Physiol. Gastrointest. Liver Physiol. 2007, 293, G256–G263. [Google Scholar] [CrossRef] [Green Version]
  36. Hofmann, A.F.; Hagey, L.R.; Krasowski, M.D. Bile salts of vertebrates: Structural variation and possible evolutionary significance. J. Lipid Res. 2010, 51, 226–246. [Google Scholar] [CrossRef] [Green Version]
  37. Fiorucci, S.; Mencarelli, A.; Palladino, G.; Cipriani, S. Bile-acid-activated receptors: Targeting TGR5 and farnesoid-X-receptor in lipid and glucose disorders. Trends Pharm. Sci. 2009, 30, 570–580. [Google Scholar] [CrossRef]
  38. Wang, H.; Chen, J.; Hollister, K.; Sowers, L.C.; Forman, B.M. Endogenous bile acids are ligands for the nuclear receptor FXR/BAR. Mol. Cell 1999, 3, 543–553. [Google Scholar] [CrossRef]
  39. Maruyama, T.; Miyamoto, Y.; Nakamura, T.; Tamai, Y.; Okada, H.; Sugiyama, E.; Nakamura, T.; Itadani, H.; Tanaka, K. Identification of membrane-type receptor for bile acids (M-BAR). Biochem. Biophys. Res. Commun. 2002, 298, 714–719. [Google Scholar] [CrossRef]
  40. Kawamata, Y.; Fujii, R.; Hosoya, M.; Harada, M.; Yoshida, H.; Miwa, M.; Fukusumi, S.; Habata, Y.; Itoh, T.; Shintani, Y.; et al. A G protein-coupled receptor responsive to bile acids. J. Biol. Chem. 2003, 278, 9435–9440. [Google Scholar] [CrossRef] [Green Version]
  41. Ridlon, J.M.; Wolf, P.G.; Gaskins, H.R. Taurocholic acid metabolism by gut microbes and colon cancer. Gut Microbes 2016, 7, 201–215. [Google Scholar] [CrossRef] [Green Version]
  42. Song, M.; Yang, Q.; Zhang, F.L.; Chen, L.; Su, H.; Yang, X.H.; He, H.W.; Liu, F.F.; Zheng, J.S.; Ling, M.F.; et al. Hyodeoxycholic acid (HDCA) suppresses intestinal epithelial cell proliferation through FXR-PI3K/AKT pathway, accompanied by alteration of bile acids metabolism profiles induced by gut bacteria. Faseb. J. 2020, 34, 7103–7117. [Google Scholar] [CrossRef] [PubMed]
  43. Qi, X.Y.; Yun, C.Y.; Sun, L.L.; Xia, J.L.; Wu, Q.; Wang, Y.; Wang, L.N.; Zhang, Y.M.; Liang, X.Y.; Wang, L.Y.; et al. Gut microbiota-bile acid-interleukin-22 axis orchestrates polycystic ovary syndrome. Nat. Med. 2019, 25, 1225–1233. [Google Scholar] [CrossRef] [PubMed]
  44. Marksteiner, J.; Blasko, I.; Kemmler, G.; Koal, T.; Humpel, C. Bile acid quantification of 20 plasma metabolites identifies lithocholic acid as a putative biomarker in Alzheimer’s disease. Metabolomics 2018, 14. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Wang, W.J.; Zhao, J.F.; Gui, W.F.; Sun, D.; Dai, H.J.; Xiao, L.; Chu, H.K.; Du, F.; Zhu, Q.J.; Schnabl, B.; et al. Tauroursodeoxycholic acid inhibits intestinal inflammation and barrier disruption in mice with non-alcoholic fatty liver disease. Br. J. Pharm. 2018, 175, 469–484. [Google Scholar] [CrossRef] [Green Version]
  46. Vandewynckel, Y.P.; Laukens, D.; Devisscher, L.; Paridaens, A.; Bogaerts, E.; Verhelst, X.; Van den Bussche, A.; Raevens, S.; Van Steenkiste, C.; Van Troys, M.; et al. Tauroursodeoxycholic Acid Dampens Oncogenic Apoptosis Induced by Endoplasmic Reticulum Stress during Hepatocarcinogen Exposure. J. Hepatol. 2015, 62, S402–S403. [Google Scholar] [CrossRef]
  47. He, J.; Liang, J.R.; Zhu, S.; Zhao, W.N.; Zhang, Y.M.; Sun, W.J. Protective effect of taurohyodeoxycholic acid from Pulvis Fellis Suis on trinitrobenzene sulfonic acid induced ulcerative colitis in mice. Eur. J. Pharm. 2011, 670, 229–235. [Google Scholar] [CrossRef]
  48. Li, F.; Jiang, C.T.; Krausz, K.W.; Li, Y.F.; Albert, I.; Hao, H.P.; Fabre, K.M.; Mitchell, J.B.; Patterson, A.D.; Gonzalez, F.J. Microbiome remodelling leads to inhibition of intestinal farnesoid X receptor signalling and decreased obesity. Nat. Commun. 2013, 4, 2384. [Google Scholar] [CrossRef]
  49. Pan, X.B.; Elliott, C.T.; McGuinness, B.; Passmore, P.; Kehoe, P.G.; Holscher, C.; McClean, P.L.; Graham, S.F.; Green, B.D. Metabolomic Profiling of Bile Acids in Clinical and Experimental Samples of Alzheimer’s Disease. Metabolites 2017, 7, 28. [Google Scholar] [CrossRef] [Green Version]
  50. McGlone, E.R.; Bloom, S.R. Bile acids and the metabolic syndrome. Ann. Clin. Biochem. 2019, 56, 326–337. [Google Scholar] [CrossRef]
  51. Fiorucci, S.; Biagioli, M.; Zampella, A.; Distrutti, E. Bile Acids Activated Receptors Regulate innate immunity. Front. Immunol. 2018, 9, 1853. [Google Scholar] [CrossRef] [Green Version]
  52. Wammers, M.; Schupp, A.K.; Bode, J.G.; Ehlting, C.; Wolf, S.; Deenen, R.; Kohrer, K.; Haussinger, D.; Graf, D. Reprogramming of pro-inflammatory human macrophages to an anti-inflammatory phenotype by bile acids. Sci. Rep. 2018, 8, 255. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  53. Biagioli, M.; Carino, A.; Cipriani, S.; Francisci, D.; Marchiano, S.; Scarpelli, P.; Sorcini, D.; Zampella, A.; Fiorucci, S. The Bile Acid Receptor GPBAR1 Regulates the M1/M2 Phenotype of Intestinal Macrophages and Activation of GPBAR1 Rescues Mice from Murine Colitis. J. Immunol. 2017, 199, 718–733. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  54. Begley, M.; Gahan, C.G.; Hill, C. The interaction between bacteria and bile. Fems. Microbiol. Rev. 2005, 29, 625–651. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  55. Arumugam, M.; Raes, J.; Pelletier, E.; Le Paslier, D.; Yamada, T.; Mende, D.R.; Fernandes, G.R.; Tap, J.; Bruls, T.; Batto, J.M.; et al. Enterotypes of the human gut microbiome. Nature 2011, 473, 174–180. [Google Scholar] [CrossRef] [PubMed]
  56. Wu, G.D.; Chen, J.; Hoffmann, C.; Bittinger, K.; Chen, Y.Y.; Keilbaugh, S.A.; Bewtra, M.; Knights, D.; Walters, W.A.; Knight, R.; et al. Linking long-term dietary patterns with gut microbial enterotypes. Science 2011, 334, 105–108. [Google Scholar] [CrossRef] [Green Version]
  57. Brestoff, J.R.; Artis, D. Commensal bacteria at the interface of host metabolism and the immune system. Nat. Immunol. 2013, 14, 676–684. [Google Scholar] [CrossRef] [Green Version]
  58. Zeng, M.Y.; Inohara, N.; Nunez, G. Mechanisms of inflammation-driven bacterial dysbiosis in the gut. Mucosal Immunol. 2017, 10, 18–26. [Google Scholar] [CrossRef] [Green Version]
  59. Molinero, N.; Ruiz, L.; Milani, C.; Gutierrez-Diaz, I.; Sanchez, B.; Mangifesta, M.; Segura, J.; Cambero, I.; Campelo, A.B.; Garcia-Bernardo, C.M.; et al. The human gallbladder microbiome is related to the physiological state and the biliary metabolic profile. Microbiome 2019, 7, 100. [Google Scholar] [CrossRef] [Green Version]
  60. Folseraas, T.; Melum, E.; Rausch, P.; Juran, B.D.; Ellinghaus, E.; Shiryaev, A.; Laerdahl, J.K.; Ellinghaus, D.; Schramm, C.; Weismuller, T.J.; et al. Extended analysis of a genome-wide association study in primary sclerosing cholangitis detects multiple novel risk loci. J. Hepatol. 2012, 57, 366–375. [Google Scholar] [CrossRef] [Green Version]
  61. Verdier, J.; Luedde, T.; Sellge, G. Biliary Mucosal Barrier and Microbiome. Viszeralmedizin 2015, 31, 156–161. [Google Scholar] [CrossRef] [Green Version]
  62. Ridlon, J.M.; Kang, D.J.; Hylemon, P.B.; Bajaj, J.S. Bile acids and the gut microbiome. Curr. Opin. Gastroenterol. 2014, 30, 332–338. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  63. Grill, J.P.; Perrin, S.; Schneider, F. Bile salt toxicity to some bifidobacteria strains: Role of conjugated bile salt hydrolase and pH. Can. J. Microbiol. 2000, 46, 878–884. [Google Scholar] [CrossRef] [PubMed]
  64. Dussurget, O.; Cabanes, D.; Dehoux, P.; Lecuit, M.; Buchrieser, C.; Glaser, P.; Cossart, P. European Listeria Genome Consortium. Listeria monocytogenes bile salt hydrolase is a PrfA-regulated virulence factor involved in the intestinal and hepatic phases of listeriosis. Mol. Microbiol 2002, 45, 1095–1106. [Google Scholar] [CrossRef]
  65. Carbonero, F.; Benefiel, A.C.; Alizadeh-Ghamsari, A.H.; Gaskins, H.R. Microbial pathways in colonic sulfur metabolism and links with health and disease. Front. Physiol. 2012, 3, 448. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  66. Out, C.; Patankar, J.V.; Doktorova, M.; Boesjes, M.; Bos, T.; de Boer, S.; Havinga, R.; Wolters, H.; Boverhof, R.; van Dijk, T.H.; et al. Gut microbiota inhibit Asbt-dependent intestinal bile acid reabsorption via Gata4. J. Hepatol. 2015, 63, 697–704. [Google Scholar] [CrossRef] [Green Version]
  67. Sayin, S.I.; Wahlstrom, A.; Felin, J.; Jantti, S.; Marschall, H.U.; Bamberg, K.; Angelin, B.; Hyotylainen, T.; Oresic, M.; Backhed, F. Gut microbiota regulates bile acid metabolism by reducing the levels of tauro-beta-muricholic acid, a naturally occurring FXR antagonist. Cell Metab. 2013, 17, 225–235. [Google Scholar] [CrossRef] [Green Version]
  68. Wahlstrom, A.; Sayin, S.I.; Marschall, H.U.; Backhed, F. Intestinal Crosstalk between Bile Acids and Microbiota and Its Impact on Host Metabolism. Cell Metab. 2016, 24, 41–50. [Google Scholar] [CrossRef] [Green Version]
  69. Zarrinpar, A.; Chaix, A.; Xu, Z.Z.; Chang, M.W.; Marotz, C.A.; Saghatelian, A.; Knight, R.; Panda, S. Antibiotic-induced microbiome depletion alters metabolic homeostasis by affecting gut signaling and colonic metabolism. Nat. Commun. 2018, 9, 2872. [Google Scholar] [CrossRef] [Green Version]
  70. Long, S.L.; Gahan, C.G.M.; Joyce, S.A. Interactions between gut bacteria and bile in health and disease. Mol. Asp. Med. 2017, 56, 54–65. [Google Scholar] [CrossRef]
  71. Urdaneta, V.; Casadesus, J. Interactions between Bacteria and Bile Salts in the Gastrointestinal and Hepatobiliary Tracts. Front. Med. (Lausanne) 2017, 4, 163. [Google Scholar] [CrossRef]
  72. Bernstein, C.; Bernstein, H.; Payne, C.M.; Beard, S.E.; Schneider, J. Bile salt activation of stress response promoters in Escherichia coli. Curr. Microbiol. 1999, 39, 68–72. [Google Scholar] [CrossRef] [PubMed]
  73. Islam, K.B.; Fukiya, S.; Hagio, M.; Fujii, N.; Ishizuka, S.; Ooka, T.; Ogura, Y.; Hayashi, T.; Yokota, A. Bile acid is a host factor that regulates the composition of the cecal microbiota in rats. Gastroenterology 2011, 141, 1773–1781. [Google Scholar] [CrossRef] [PubMed]
  74. Kakiyama, G.; Pandak, W.M.; Gillevet, P.M.; Hylemon, P.B.; Heuman, D.M.; Daita, K.; Takei, H.; Muto, A.; Nittono, H.; Ridlon, J.M.; et al. Modulation of the fecal bile acid profile by gut microbiota in cirrhosis. J. Hepatol. 2013, 58, 949–955. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  75. Inagaki, T.; Moschetta, A.; Lee, Y.K.; Peng, L.; Zhao, G.; Downes, M.; Yu, R.T.; Shelton, J.M.; Richardson, J.A.; Repa, J.J.; et al. Regulation of antibacterial defense in the small intestine by the nuclear bile acid receptor. Proc. Natl. Acad. Sci. USA 2006, 103, 3920–3925. [Google Scholar] [CrossRef] [Green Version]
  76. Vavassori, P.; Mencarelli, A.; Renga, B.; Distrutti, E.; Fiorucci, S. The bile acid receptor FXR is a modulator of intestinal innate immunity. J. Immunol. 2009, 183, 6251–6261. [Google Scholar] [CrossRef] [Green Version]
  77. Cipriani, S.; Mencarelli, A.; Chini, M.G.; Distrutti, E.; Renga, B.; Bifulco, G.; Baldelli, F.; Donini, A.; Fiorucci, S. The bile acid receptor GPBAR-1 (TGR5) modulates integrity of intestinal barrier and immune response to experimental colitis. PLoS ONE 2011, 6, e25637. [Google Scholar] [CrossRef]
  78. David, L.A.; Materna, A.C.; Friedman, J.; Campos-Baptista, M.I.; Blackburn, M.C.; Perrotta, A.; Erdman, S.E.; Alm, E.J. Host lifestyle affects human microbiota on daily timescales. Genome Biol. 2014, 15, R89. [Google Scholar] [CrossRef] [Green Version]
  79. David, L.A.; Maurice, C.F.; Carmody, R.N.; Gootenberg, D.B.; Button, J.E.; Wolfe, B.E.; Ling, A.V.; Devlin, A.S.; Varma, Y.; Fischbach, M.A.; et al. Diet rapidly and reproducibly alters the human gut microbiome. Nature 2014, 505, 559–563. [Google Scholar] [CrossRef] [Green Version]
  80. Desai, M.S.; Seekatz, A.M.; Koropatkin, N.M.; Kamada, N.; Hickey, C.A.; Wolter, M.; Pudlo, N.A.; Kitamoto, S.; Terrapon, N.; Muller, A.; et al. A Dietary Fiber-Deprived Gut Microbiota Degrades the Colonic Mucus Barrier and Enhances Pathogen Susceptibility. Cell 2016, 167, 1339–1353.e1321. [Google Scholar] [CrossRef] [Green Version]
  81. Alvarez-Ordonez, A.; Begley, M.; Prieto, M.; Messens, W.; Lopez, M.; Bernardo, A.; Hill, C. Salmonella spp. survival strategies within the host gastrointestinal tract. Microbiology 2011, 157, 3268–3281. [Google Scholar] [CrossRef] [Green Version]
  82. Devendran, S.; Shrestha, R.; Alves, J.M.P.; Wolf, P.G.; Ly, L.; Hernandez, A.G.; Mendez-Garcia, C.; Inboden, A.; Wiley, J.; Paul, O.; et al. Clostridium scindens ATCC 35704: Integration of Nutritional Requirements, the Complete Genome Sequence, and Global Transcriptional Responses to Bile Acids. Appl. Environ. Microbiol. 2019, 85. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  83. Buffie, C.G.; Bucci, V.; Stein, R.R.; McKenney, P.T.; Ling, L.; Gobourne, A.; No, D.; Liu, H.; Kinnebrew, M.; Viale, A.; et al. Precision microbiome reconstitution restores bile acid mediated resistance to Clostridium difficile. Nature 2015, 517, 205–208. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  84. Grau, K.R.; Zhu, S.; Peterson, S.T.; Helm, E.W.; Philip, D.; Phillips, M.; Hernandez, A.; Turula, H.; Frasse, P.; Graziano, V.R.; et al. The intestinal regionalization of acute norovirus infection is regulated by the microbiota via bile acid-mediated priming of type III interferon. Nat. Microbiol. 2020, 5, 84–92. [Google Scholar] [CrossRef] [PubMed]
  85. Abdulrab, S.; Al-Maweri, S.; Halboub, E. Ursodeoxycholic acid as a candidate therapeutic to alleviate and/or prevent COVID-19-associated cytokine storm. Med. Hypotheses 2020, 143, 109897. [Google Scholar] [CrossRef]
  86. Carino, A.; Moraca, F.; Fiorillo, B.; Marchiano, S.; Sepe, V.; Biagioli, M.; Finamore, C.; Bozza, S.; Francisci, D.; Distrutti, E.; et al. Hijacking SARS-CoV-2/ACE2 Receptor Interaction by Natural and Semi-synthetic Steroidal Agents Acting on Functional Pockets on the Receptor Binding Domain. Front. Chem. 2020, 8, 572885. [Google Scholar] [CrossRef]
  87. Abraham, C.; Cho, J.H. Inflammatory bowel disease. New. Engl. J. Med. 2009, 361, 2066–2078. [Google Scholar] [CrossRef]
  88. Baumgart, D.C.; Carding, S.R. Inflammatory bowel disease: Cause and immunobiology. Lancet 2007, 369, 1627–1640. [Google Scholar] [CrossRef]
  89. Engel, M.A.; Neurath, M.F. New pathophysiological insights and modern treatment of IBD. J. Gastroenterol. 2010, 45, 571–583. [Google Scholar] [CrossRef]
  90. Braun, J.; Wei, B. Body traffic: Ecology, genetics, and immunity in inflammatory bowel disease. Annu. Rev. Pathol. 2007, 2, 401–429. [Google Scholar] [CrossRef] [Green Version]
  91. Connors, J.; Dunn, K.A.; Allott, J.; Bandsma, R.; Rashid, M.; Otley, A.R.; Bielawski, J.P.; Van Limbergen, J. The relationship between fecal bile acids and microbiome community structure in pediatric Crohn’s disease. ISME J. 2020, 14, 702–713. [Google Scholar] [CrossRef]
  92. Dawson, P.A.; Karpen, S.J. Intestinal transport and metabolism of bile acids. J. Lipid Res. 2015, 56, 1085–1099. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  93. Pavlidis, P.; Powell, N.; Vincent, R.P.; Ehrlich, D.; Bjarnason, I.; Hayee, B. Systematic review: Bile acids and intestinal inflammation-luminal aggressors or regulators of mucosal defence? Aliment. Pharm. 2015, 42, 802–817. [Google Scholar] [CrossRef] [PubMed]
  94. Rau, M.; Stieger, B.; Monte, M.J.; Schmitt, J.; Jahn, D.; Frey-Wagner, I.; Raselli, T.; Marin, J.J.; Mullhaupt, B.; Rogler, G.; et al. Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation. Inflamm. Bowel Dis. 2016, 22, 2382–2389. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  95. Guard, B.C.; Honneffer, J.B.; Jergens, A.E.; Jonika, M.M.; Toresson, L.; Lawrence, Y.A.; Webb, C.B.; Hill, S.; Lidbury, J.A.; Steiner, J.M.; et al. Longitudinal assessment of microbial dysbiosis, fecal unconjugated bile acid concentrations, and disease activity in dogs with steroid-responsive chronic inflammatory enteropathy. J. Vet. Intern. Med. 2019, 33, 1295–1305. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  96. Jahnel, J.; Fickert, P.; Hauer, A.C.; Hogenauer, C.; Avian, A.; Trauner, M. Inflammatory bowel disease alters intestinal bile acid transporter expression. Drug Metab. Dispos. 2014, 42, 1423–1431. [Google Scholar] [CrossRef] [PubMed]
  97. Jung, D.; Fantin, A.C.; Scheurer, U.; Fried, M.; Kullak-Ublick, G.A. Human ileal bile acid transporter gene ASBT (SLC10A2) is transactivated by the glucocorticoid receptor. Gut 2004, 53, 78–84. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  98. Song, X.; Sun, X.; Oh, S.F.; Wu, M.; Zhang, Y.; Zheng, W.; Geva-Zatorsky, N.; Jupp, R.; Mathis, D.; Benoist, C.; et al. Microbial bile acid metabolites modulate gut RORgamma+ regulatory T cell homeostasis. Nature 2020, 577, 410–415. [Google Scholar] [CrossRef]
  99. Paramsothy, S.; Nielsen, S.; Kamm, M.A.; Deshpande, N.P.; Faith, J.J.; Clemente, J.C.; Paramsothy, R.; Walsh, A.J.; van den Bogaerde, J.; Samuel, D.; et al. Specific Bacteria and Metabolites Associated With Response to Fecal Microbiota Transplantation in Patients With Ulcerative Colitis. Gastroenterology 2019, 156, 1440–1454 e1442. [Google Scholar] [CrossRef] [Green Version]
  100. Mattner, J. Impact of Microbes on the Pathogenesis of Primary Biliary Cirrhosis (PBC) and Primary Sclerosing Cholangitis (PSC). Int. J. Mol. Sci. 2016, 17, 1864. [Google Scholar] [CrossRef]
  101. Liwinski, T.; Zenouzi, R.; John, C.; Ehlken, H.; Ruhlemann, M.C.; Bang, C.; Groth, S.; Lieb, W.; Kantowski, M.; Andersen, N.; et al. Alterations of the bile microbiome in primary sclerosing cholangitis. Gut 2020, 69, 665–672. [Google Scholar] [CrossRef] [Green Version]
  102. Ruhlemann, M.C.; Solovjeva, M.E.L.; Zenouzi, R.; Liwinski, T.; Kummen, M.; Lieb, W.; Hov, J.R.; Schramm, C.; Franke, A.; Bang, C. Gut mycobiome of primary sclerosing cholangitis patients is characterised by an increase of Trichocladium griseum and Candida species. Gut 2020, 69, 1890–1892. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  103. Miyake, Y.; Yamamoto, K. Role of gut microbiota in liver diseases. Hepatol. Res. 2013, 43, 139–146. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  104. Pflughoeft, K.J.; Versalovic, J. Human microbiome in health and disease. Annu. Rev. Pathol. 2012, 7, 99–122. [Google Scholar] [CrossRef] [PubMed]
  105. Mattner, J.; Savage, P.B.; Leung, P.; Oertelt, S.S.; Wang, V.; Trivedi, O.; Scanlon, S.T.; Pendem, K.; Teyton, L.; Hart, J.; et al. Liver autoimmunity triggered by microbial activation of natural killer T cells. Cell Host Microbe 2008, 3, 304–315. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  106. Mohammed, J.P.; Fusakio, M.E.; Rainbow, D.B.; Moule, C.; Fraser, H.I.; Clark, J.; Todd, J.A.; Peterson, L.B.; Savage, P.B.; Wills-Karp, M.; et al. Identification of Cd101 as a susceptibility gene for Novosphingobium aromaticivorans-induced liver autoimmunity. J. Immunol. 2011, 187, 337–349. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  107. Eaton, J.E.; Talwalkar, J.A.; Lazaridis, K.N.; Gores, G.J.; Lindor, K.D. Pathogenesis of primary sclerosing cholangitis and advances in diagnosis and management. Gastroenterology 2013, 145, 521–536. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  108. Torres, J.; Palmela, C.; Brito, H.; Bao, X.; Ruiqi, H.; Moura-Santos, P.; da Silva, J.P.; Oliveira, A.; Vieira, C.; Perez, K.; et al. The gut microbiota, bile acids and their correlation in primary sclerosing cholangitis associated with inflammatory bowel disease. United Eur. Gastroenterol. J. 2018, 6, 112–122. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  109. Rossen, N.G.; Fuentes, S.; Boonstra, K.; D’Haens, G.R.; Heilig, H.G.; Zoetendal, E.G.; de Vos, W.M.; Ponsioen, C.Y. The mucosa-associated microbiota of PSC patients is characterized by low diversity and low abundance of uncultured Clostridiales II. J. Crohns Colitis 2015, 9, 342–348. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  110. Lichtman, S.N.; Okoruwa, E.E.; Keku, J.; Schwab, J.H.; Sartor, R.B. Degradation of endogenous bacterial cell wall polymers by the muralytic enzyme mutanolysin prevents hepatobiliary injury in genetically susceptible rats with experimental intestinal bacterial overgrowth. J. Clin. Investig. 1992, 90, 1313–1322. [Google Scholar] [CrossRef] [Green Version]
  111. Tabibian, J.H.; Talwalkar, J.A.; Lindor, K.D. Role of the microbiota and antibiotics in primary sclerosing cholangitis. Biomed. Res. Int. 2013, 2013, 389537. [Google Scholar] [CrossRef] [Green Version]
  112. Weingarden, A.R.; Chen, C.; Zhang, N.; Graiziger, C.T.; Dosa, P.I.; Steer, C.J.; Shaughnessy, M.K.; Johnson, J.R.; Sadowsky, M.J.; Khoruts, A. Ursodeoxycholic Acid Inhibits Clostridium difficile Spore Germination and Vegetative Growth, and Prevents the Recurrence of Ileal Pouchitis Associated With the Infection. J. Clin. Gastroenterol. 2016, 50, 624–630. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  113. Pushalkar, S.; Hundeyin, M.; Daley, D.; Zambirinis, C.P.; Kurz, E.; Mishra, A.; Mohan, N.; Aykut, B.; Usyk, M.; Torres, L.E.; et al. The Pancreatic Cancer Microbiome Promotes Oncogenesis by Induction of Innate and Adaptive Immune Suppression. Cancer Discov. 2018, 8, 403–416. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  114. Finlay, B.B.; Goldszmid, R.; Honda, K.; Trinchieri, G.; Wargo, J.; Zitvogel, L. Can we harness the microbiota to enhance the efficacy of cancer immunotherapy? Nat. Rev. Immunol. 2020, 20, 522–528. [Google Scholar] [CrossRef] [PubMed]
  115. Vetizou, M.; Pitt, J.M.; Daillere, R.; Lepage, P.; Waldschmitt, N.; Flament, C.; Rusakiewicz, S.; Routy, B.; Roberti, M.P.; Duong, C.P.; et al. Anticancer immunotherapy by CTLA-4 blockade relies on the gut microbiota. Science 2015, 350, 1079–1084. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  116. Routy, B.; Le Chatelier, E.; Derosa, L.; Duong, C.P.M.; Alou, M.T.; Daillere, R.; Fluckiger, A.; Messaoudene, M.; Rauber, C.; Roberti, M.P.; et al. Gut microbiome influences efficacy of PD-1-based immunotherapy against epithelial tumors. Science 2018, 359, 91–97. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  117. Anker, J.F.; Naseem, A.F.; Mok, H.; Schaeffer, A.J.; Abdulkadir, S.A.; Thumbikat, P. Multi-faceted immunomodulatory and tissue-tropic clinical bacterial isolate potentiates prostate cancer immunotherapy. Nat. Commun. 2018, 9, 1591. [Google Scholar] [CrossRef]
  118. Wang, S.; Dong, W.; Liu, L.; Xu, M.; Wang, Y.; Liu, T.; Zhang, Y.; Wang, B.; Cao, H. Interplay between bile acids and the gut microbiota promotes intestinal carcinogenesis. Mol. Carcinog. 2019, 58, 1155–1167. [Google Scholar] [CrossRef] [Green Version]
  119. Hassan, H.M.; Isovic, M.; Kolendowski, B.; Bauer-Maison, N.; Onabote, O.; Cecchini, M.; Haig, A.; Maleki Vareki, S.; Underhill, T.M.; Torchia, J. Loss of Thymine DNA Glycosylase Causes Dysregulation of Bile Acid Homeostasis and Hepatocellular Carcinoma. Cell Rep. 2020, 31, 107475. [Google Scholar] [CrossRef]
  120. Phelan, J.P.; Reen, F.J.; Caparros-Martin, J.A.; O’Connor, R.; O’Gara, F. Rethinking the bile acid/gut microbiome axis in cancer. Oncotarget 2017, 8, 115736–115747. [Google Scholar] [CrossRef] [Green Version]
  121. Ren, X.H.; Xu, J.; Zhang, Y.Y.; Chen, G.D.; Zhang, Y.W.; Huang, Q.; Liu, Y.L. Bacterial Alterations in Post-Cholecystectomy Patients Are Associated With Colorectal Cancer. Front. Oncol. 2020, 10, 1418. [Google Scholar] [CrossRef]
  122. Yoon, W.J.; Kim, H.N.; Park, E.; Ryu, S.; Chang, Y.; Shin, H.; Kim, H.L.; Yi, S.Y. The Impact of Cholecystectomy on the Gut Microbiota: A Case-Control Study. J. Clin. Med. 2019, 8, 79. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  123. Wang, W.X.; Wang, J.F.; Li, J.L.; Yan, P.P.; Jin, Y.; Zhang, R.Y.; Yue, W.; Guo, Q.; Geng, J.W. Cholecystectomy Damages Aging-Associated Intestinal Microbiota Construction. Front. Microbiol. 2018, 9, 1402. [Google Scholar] [CrossRef] [PubMed]
  124. Sarashina-Kida, H.; Negishi, H.; Nishio, J.; Suda, W.; Nakajima, Y.; Yasui-Kato, M.; Iwaisako, K.; Kang, S.J.; Endo, N.; Yanai, H.; et al. Gallbladder-derived surfactant protein D regulates gut commensal bacteria for maintaining intestinal homeostasis. Proc. Natl. Acad. Sci. USA 2017, 114, 10178–10183. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  125. Cao, H.; Xu, M.; Dong, W.; Deng, B.; Wang, S.; Zhang, Y.; Wang, S.; Luo, S.; Wang, W.; Qi, Y.; et al. Secondary bile acid-induced dysbiosis promotes intestinal carcinogenesis. Int. J. Cancer 2017, 140, 2545–2556. [Google Scholar] [CrossRef] [Green Version]
  126. Hale, V.L.; Chen, J.; Johnson, S.; Harrington, S.C.; Yab, T.C.; Smyrk, T.C.; Nelson, H.; Boardman, L.A.; Druliner, B.R.; Levin, T.R.; et al. Shifts in the Fecal Microbiota Associated with Adenomatous Polyps. Cancer Epidemiol. Biomark. Prev. 2017, 26, 85–94. [Google Scholar] [CrossRef] [Green Version]
  127. Chen, D.; Jin, D.; Huang, S.; Wu, J.; Xu, M.; Liu, T.; Dong, W.; Liu, X.; Wang, S.; Zhong, W.; et al. Clostridium butyricum, a butyrate-producing probiotic, inhibits intestinal tumor development through modulating Wnt signaling and gut microbiota. Cancer Lett. 2020, 469, 456–467. [Google Scholar] [CrossRef]
  128. Zeng, H.; Safratowich, B.D.; Wang, T.T.Y.; Hamlin, S.K.; Johnson, L.K. Butyrate Inhibits Deoxycholic-Acid-Resistant Colonic Cell Proliferation via Cell Cycle Arrest and Apoptosis: A Potential Pathway Linking Dietary Fiber to Cancer Prevention. Mol. Nutr. Food Res. 2020, 64, e1901014. [Google Scholar] [CrossRef]
  129. Mao, J.; Chen, X.; Wang, C.; Li, W.; Li, J. Effects and mechanism of the bile acid (farnesoid X) receptor on the Wnt/beta-catenin signaling pathway in colon cancer. Oncol. Lett. 2020, 20, 337–345. [Google Scholar] [CrossRef] [Green Version]
  130. Coutzac, C.; Jouniaux, J.M.; Paci, A.; Schmidt, J.; Mallardo, D.; Seck, A.; Asvatourian, V.; Cassard, L.; Saulnier, P.; Lacroix, L.; et al. Systemic short chain fatty acids limit antitumor effect of CTLA-4 blockade in hosts with cancer. Nat. Commun. 2020, 11, 2168. [Google Scholar] [CrossRef]
  131. Mattner, J. Natural killer T (NKT) cells in autoimmune hepatitis. Curr. Opin. Immunol. 2013, 25, 697–703. [Google Scholar] [CrossRef] [Green Version]
  132. Sydor, S.; Best, J.; Messerschmidt, I.; Manka, P.; Vilchez-Vargas, R.; Brodesser, S.; Lucas, C.; Wegehaupt, A.; Wenning, C.; Assmuth, S.; et al. Altered Microbiota Diversity and Bile Acid Signaling in Cirrhotic and Noncirrhotic NASH-HCC. Clin. Transl. Gastroenterol. 2020, 11, e00131. [Google Scholar] [CrossRef] [PubMed]
  133. Yamada, S.; Takashina, Y.; Watanabe, M.; Nagamine, R.; Saito, Y.; Kamada, N.; Saito, H. Bile acid metabolism regulated by the gut microbiota promotes non-alcoholic steatohepatitis-associated hepatocellular carcinoma in mice. Oncotarget 2018, 9, 9925–9939. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  134. Xie, G.; Wang, X.; Zhao, A.; Yan, J.; Chen, W.; Jiang, R.; Ji, J.; Huang, F.; Zhang, Y.; Lei, S.; et al. Sex-dependent effects on gut microbiota regulate hepatic carcinogenic outcomes. Sci. Rep. 2017, 7, 45232. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  135. Cassano, M.; Offner, S.; Planet, E.; Piersigilli, A.; Jang, S.M.; Henry, H.; Geuking, M.B.; Mooser, C.; McCoy, K.D.; Macpherson, A.J.; et al. Polyphenic trait promotes liver cancer in a model of epigenetic instability in mice. Hepatology 2017, 66, 235–251. [Google Scholar] [CrossRef]
  136. Kohli, R.M.; Zhang, Y. TET enzymes, TDG and the dynamics of DNA demethylation. Nature 2013, 502, 472–479. [Google Scholar] [CrossRef] [Green Version]
  137. Cho, I.; Blaser, M.J. The human microbiome: At the interface of health and disease. Nat. Rev. Genet. 2012, 13, 260–270. [Google Scholar] [CrossRef] [Green Version]
  138. Cho, I.; Yamanishi, S.; Cox, L.; Methe, B.A.; Zavadil, J.; Li, K.; Gao, Z.; Mahana, D.; Raju, K.; Teitler, I.; et al. Antibiotics in early life alter the murine colonic microbiome and adiposity. Nature 2012, 488, 621–626. [Google Scholar] [CrossRef]
Ijms 22 01397 g001 550
Figure 1. Schematic overview of the enterohepatic circulation (EHC). Primary bile acids (BAs) are synthesized in the liver and secreted into the duodenum with the bile; 95% of the BAs are re-absorbed in the terminal ileum and transported back to the liver for recycling. The remaining 5% enter the colon, where they are transformed into secondary BAs by colonic microbiota.
Figure 1. Schematic overview of the enterohepatic circulation (EHC). Primary bile acids (BAs) are synthesized in the liver and secreted into the duodenum with the bile; 95% of the BAs are re-absorbed in the terminal ileum and transported back to the liver for recycling. The remaining 5% enter the colon, where they are transformed into secondary BAs by colonic microbiota.
Ijms 22 01397 g001
Ijms 22 01397 g002 550
Figure 2. Effects of bile acid (BA) receptor engagement on immune cell function. After binding to the farnesoid X receptor (FXR) or the G protein-coupled receptor (TGR5), primary and secondary BAs shift the cytokine profile of myeloid cells to an anti-inflammatory phenotype. Their agonistic functions thereby depend on the affinity of individual BAs for the respective receptor, as indicated in the figure [51]. (IL-10 = interleukin-10; TGF-β = transforming growth factor beta; IFNγ = interferon gamma; TNF-α = tumor necrosis factor alpha; IL-6 = interleukin-6; IL-1β = interleukin-1 beta).
Figure 2. Effects of bile acid (BA) receptor engagement on immune cell function. After binding to the farnesoid X receptor (FXR) or the G protein-coupled receptor (TGR5), primary and secondary BAs shift the cytokine profile of myeloid cells to an anti-inflammatory phenotype. Their agonistic functions thereby depend on the affinity of individual BAs for the respective receptor, as indicated in the figure [51]. (IL-10 = interleukin-10; TGF-β = transforming growth factor beta; IFNγ = interferon gamma; TNF-α = tumor necrosis factor alpha; IL-6 = interleukin-6; IL-1β = interleukin-1 beta).
Ijms 22 01397 g002
Ijms 22 01397 g003 550
Figure 3. Influence of bile acid (BA) composition on colonic cell responses to the toxin of Bacteroides fragilis. The disruption of intestinal microbiota reduces the accumulation of primary BAs in the gut. Subsequently, the protective effect of primary BAs on colonic cell responses is lost and renders these cells susceptible to damage upon exposure to the toxin of Bacteroides fragilis.
Figure 3. Influence of bile acid (BA) composition on colonic cell responses to the toxin of Bacteroides fragilis. The disruption of intestinal microbiota reduces the accumulation of primary BAs in the gut. Subsequently, the protective effect of primary BAs on colonic cell responses is lost and renders these cells susceptible to damage upon exposure to the toxin of Bacteroides fragilis.
Ijms 22 01397 g003
Ijms 22 01397 g004 550
Figure 4. Inhibition of liver cell growth through natural killer T (NKT) cells. Chenodeoxycholic acid (CDCA) enhances the expression of CXCL16 on liver sinusoidal endothelial cells. Subsequently, CXCR6+-NKT-cells accumulate in the liver and protect against hepatocellular carcinoma (HCC).
Figure 4. Inhibition of liver cell growth through natural killer T (NKT) cells. Chenodeoxycholic acid (CDCA) enhances the expression of CXCL16 on liver sinusoidal endothelial cells. Subsequently, CXCR6+-NKT-cells accumulate in the liver and protect against hepatocellular carcinoma (HCC).
Ijms 22 01397 g004
Table
Table 1. Primary bile acids and their functions under physiological and pathophysiological conditions. (UC = ulcerative colitis, CRC = colorectal cancer, HCC = hepatocellular carcinoma).
Table 1. Primary bile acids and their functions under physiological and pathophysiological conditions. (UC = ulcerative colitis, CRC = colorectal cancer, HCC = hepatocellular carcinoma).
AbbrevationBile Acid NamePhysiologic FunctionsPathophysiologic Functions
CACholic acidfacilitate digestion [14];
emulsify hydrophobic food components such as fats into micelles for intestinal absorption [14];
protect from CRC induced by Bacteroides fragilis toxin [15]		favor infections with pathogenic bacteria (Salmonella spp., E. coli, Shigella dysenteriae) [16,17,18,19,20,21,22,23] and the germination of C. difficile spores [24,25,26];
can cause colitis and diarrhea in UC patients [27];
promote the accumu-lation of CXCR6+-NKT-cells in the liver and protects from HCC [28]
CDCAChenodeoxycholic acid
GCAGlycocholic acid
GCDCAGlycochenodeoxycholic acid
TCATaurocholic acid
TCDCATaurochenodeoxycholic acid
Table
Table 2. Secondary bile acids and their (patho-)physiologic functions (CRC = colorectal cancer, PBS = primary biliary cirrhosis, ILC3 = type 3 innate lymphoid cells, PSC = primary sclerosing cholangitis, NASH = non-alcoholic steatohepatitis).
Table 2. Secondary bile acids and their (patho-)physiologic functions (CRC = colorectal cancer, PBS = primary biliary cirrhosis, ILC3 = type 3 innate lymphoid cells, PSC = primary sclerosing cholangitis, NASH = non-alcoholic steatohepatitis).
AbbrevationBile Acid NamePhysiologic FunctionsPathophysiologic Functions
DCADeoxycholic acidexhibit antimicrobial activity [32];
maintain colonic microbiota [32];
perpetuate endocrine functions via binding to nuclear factor X receptor (FXR) and G-protein-coupled bile acid receptor (TGR5)
[37,38,39,40]		enhanced levels are associated with CRC development [41]
UDCAUrsodeoxycholic aciddrug for the treatment of primary biliary cirrhosis (PBC)
HDCAHyodeoxycholic acidsuppresses intestinal cell proliferation and enhances abundance of microbiota [42]
GDCAGlycodeoxycholic acidinduces interleukin-22 production by ILC3s and improves ovulatory dysfunction and insulin resistance in patients suffering from polycystic ovary syndrome (POCS) [43]
GUDCAGlycoursodeoxycholic acidneuroprotective agent (https://pubchem.ncbi.nlm.nih.gov/compound/Glycoursodeoxycholic-acid)
LCALithocholic acidenhanced levels in patients suffering from Alzheimer´s disease [44]
GLCAGlycolithocholic acid
TLCATaurolithocholic acidinduces biliary dysbiosis in PSC patients
TDCATaurodeoxycholic acid
TUDCATauroursodeoxycholic acidprotective effects in NASH [45];
associated with attenuated hepatocarcinogenesis [46]
THDCATaurohyodeoxycholic acidprotective effects in experimentally induced colitis [47]
TMCA (a+b)Tauromuricholic acid (alpha + beta)maintains lipid and glucose metabolism [48];
reduced levels in the plasma of APP/PS1 mice, which are a mouse model for Alzheimer’s disease [49]
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Grüner, N.; Mattner, J. Bile Acids and Microbiota: Multifaceted and Versatile Regulators of the Liver–Gut Axis. Int. J. Mol. Sci. 2021, 22, 1397. https://doi.org/10.3390/ijms22031397

AMA Style

Grüner N, Mattner J. Bile Acids and Microbiota: Multifaceted and Versatile Regulators of the Liver–Gut Axis. International Journal of Molecular Sciences. 2021; 22(3):1397. https://doi.org/10.3390/ijms22031397

Chicago/Turabian Style

Grüner, Niklas, and Jochen Mattner. 2021. "Bile Acids and Microbiota: Multifaceted and Versatile Regulators of the Liver–Gut Axis" International Journal of Molecular Sciences 22, no. 3: 1397. https://doi.org/10.3390/ijms22031397

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop