Combined Analysis of the Metabolome and Transcriptome Identified Candidate Genes Involved in Phenolic Acid Biosynthesis in the Leaves of Cyclocarya paliurus
Abstract
:1. Introduction
2. Results
2.1. Expression of Phenolic Acid Biosynthesis Genes in Leaves of C. Paliurus at Different Developmental Stages
2.2. Identified Metabolites Involved in Phenolic Acid Biosynthesis Pathway
2.3. Comprehensive Analysis of Metabolome and Transcriptome
2.4. Screening of Transcription Factors Related to Phenolic Acid Biosynthesis and Phylogenetic Analysis
2.5. Regulatory Network of Phenolic Acid Biosynthesis
2.6. RT-qPCR Validation of the Transcriptomic Data
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Metabolite Extraction, Multiple Reactions Monitoring and Parameter Setting
4.3. Transciptomic Analysis
4.4. Differentially Expressed Genes Analysis
4.5. Real-Time Quantitative PCR
4.6. Transcription Factors Prediction and Analysis
4.7. Statistical Analysis
4.8. Accession Numbers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Compounds | Molecular Weight (Da) | CPS | |||
---|---|---|---|---|---|
F1 | F2 | F3 | F4 | ||
Sinapoyl malate | 340.1 | 180000 ± 80600b | 85400 ± 36800b | 74600 ± 14700b | 441000 ± 97800a |
Caffeic aldehyde | 164.1 | 175000 ± 11600a | 238000 ± 72700a | 237000 ± 26300a | 196000 ± 44500a |
L-Phenylalanine | 165.079 | 25700000 ± 1560000b | 26600000 ± 5000000b | 34200000 ± 3860000a | 3200000 ± 647000c |
Cinnamic acid | 148.0524 | 71000 ± 13900a | 98900 ± 16200a | 79700 ± 16800a | 126000 ± 77500a |
Caffeic acid | 180.0423 | 378000 ± 40700b | 772000 ± 384000ab | 608000 ± 47600ab | 1700000 ± 891000a |
Ferulic acid | 194.0579 | 8430000 ± 6070000a | 7460000 ± 3860000a | 9710000 ± 4460000a | 9460000 ± 3640000a |
Chlorogenic acid | 354.0951 | 3060000 ± 237000a | 2600000 ± 318000a | 2520000 ± 267000a | 1800000 ± 146000b |
Coniferylaldehyde | 178.063 | 2090000 ± 622000b | 1930000 ± 470000b | 3050000 ± 106000a | 1880000 ± 264000b |
Syringin | 372.142 | 13300 ± 15000b | 60300 ± 21800b | 33500 ± 23700b | 118000 ± 32500a |
p-Coumaric acid | 164.047 | 368000 ± 131000a | 295000 ± 180000a | 84700 ± 34900a | 591000 ± 598000a |
Coniferyl alcohol | 180.079 | 96900 ± 48600b | 110000 ± 39100b | 65700 ± 15700b | 502000 ± 54300a |
Sinapic acid | 224.069 | 15500 ± 7880b | 41200 ± 25300b | 19000 ± 12900b | 457000 ± 183000a |
Sinapyl alcohol | 210.089 | 6040 ± 412b | 8300 ± 6100b | 1180 ± 1660b | 103000 ± 8870a |
Coniferin | 342.132 | 1660000 ± 2350000a | 21900000 ± 17300000a | 5200000 ± 7350000a | 44700000 ± 40500000a |
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Lin, W.; Li, Y.; Lu, Q.; Lu, H.; Li, J. Combined Analysis of the Metabolome and Transcriptome Identified Candidate Genes Involved in Phenolic Acid Biosynthesis in the Leaves of Cyclocarya paliurus. Int. J. Mol. Sci. 2020, 21, 1337. https://doi.org/10.3390/ijms21041337
Lin W, Li Y, Lu Q, Lu H, Li J. Combined Analysis of the Metabolome and Transcriptome Identified Candidate Genes Involved in Phenolic Acid Biosynthesis in the Leaves of Cyclocarya paliurus. International Journal of Molecular Sciences. 2020; 21(4):1337. https://doi.org/10.3390/ijms21041337
Chicago/Turabian StyleLin, Weida, Yueling Li, Qiuwei Lu, Hongfei Lu, and Junmin Li. 2020. "Combined Analysis of the Metabolome and Transcriptome Identified Candidate Genes Involved in Phenolic Acid Biosynthesis in the Leaves of Cyclocarya paliurus" International Journal of Molecular Sciences 21, no. 4: 1337. https://doi.org/10.3390/ijms21041337