Challenges in Exosome Isolation and Analysis in Health and Disease
Abstract
:1. Introduction
2. Influence of Exosome Isolation/Purification Methodologies on the Composition of Exosome Cargos
3. Culture Conditions Influence Exosome Levels and Cargo
3.1. Culture Containers
3.2. Cells
3.3. Medium Composition
3.4. Hypoxia
3.5. Treatments/Perturbations of Cells
4. Disease-Associated Alterations in Exosome Production/Cargo
Effects of Cancer Therapeutics on EV Production, Content and Function
5. Conclusions
Funding
Conflicts of Interest
References
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Ludwig, N.; Whiteside, T.L.; Reichert, T.E. Challenges in Exosome Isolation and Analysis in Health and Disease. Int. J. Mol. Sci. 2019, 20, 4684. https://doi.org/10.3390/ijms20194684
Ludwig N, Whiteside TL, Reichert TE. Challenges in Exosome Isolation and Analysis in Health and Disease. International Journal of Molecular Sciences. 2019; 20(19):4684. https://doi.org/10.3390/ijms20194684
Chicago/Turabian StyleLudwig, Nils, Theresa L. Whiteside, and Torsten E. Reichert. 2019. "Challenges in Exosome Isolation and Analysis in Health and Disease" International Journal of Molecular Sciences 20, no. 19: 4684. https://doi.org/10.3390/ijms20194684