TRAIL/NF-κB/CX3CL1 Mediated Onco-Immuno Crosstalk Leading to TRAIL Resistance of Pancreatic Cancer Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Chemokine CX3CL1 is a RelA Target Gene
2.2. RelA Binds to CX3CL1 Promoter after TRAIL Treatment
2.3. CX3CL1 Does Not Induce Direct Apoptosis in Panc1 Cells
2.4. CX3CL1 Enhances the Migration of PBMCs towards PDAC Cells
2.5. CX3CL1 Attracted PBMCs Interfere with TRAIL Mediated Apoptosis in Panc1 Cells
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. RNA Preparation and Realtime PCR
4.4. siRNA Transfection
4.5. Gel Shift Assays
4.6. Genome Wide Transcriptome Profiling and Cluster Analysis
4.7. PBMC Isolation
4.8. Migration Assay
4.9. Co-Culture Assay
4.10. ELISA
4.11. Western Blotting
4.12. Annexin V/PI Assay
4.13. Caspase-3/-7 Assay
4.14. Statistics
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PDAC | pancreatic ductal adenocarcinoma |
TRAIL | TNF alpha related apoptosis inducing ligand |
NF-κB | nuclear factor kappa-light-chain-enhancer of activated B cells |
CX3CL1 | chemokine (C-X3-C motif) ligand 1 |
CX3CR1 | CX3C chemokine receptor 1 |
RelA | nuclear factor NF-kappa-B p65 subunit |
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Geismann, C.; Erhart, W.; Grohmann, F.; Schreiber, S.; Schneider, G.; Schäfer, H.; Arlt, A. TRAIL/NF-κB/CX3CL1 Mediated Onco-Immuno Crosstalk Leading to TRAIL Resistance of Pancreatic Cancer Cell Lines. Int. J. Mol. Sci. 2018, 19, 1661. https://doi.org/10.3390/ijms19061661
Geismann C, Erhart W, Grohmann F, Schreiber S, Schneider G, Schäfer H, Arlt A. TRAIL/NF-κB/CX3CL1 Mediated Onco-Immuno Crosstalk Leading to TRAIL Resistance of Pancreatic Cancer Cell Lines. International Journal of Molecular Sciences. 2018; 19(6):1661. https://doi.org/10.3390/ijms19061661
Chicago/Turabian StyleGeismann, Claudia, Wiebke Erhart, Frauke Grohmann, Stefan Schreiber, Günter Schneider, Heiner Schäfer, and Alexander Arlt. 2018. "TRAIL/NF-κB/CX3CL1 Mediated Onco-Immuno Crosstalk Leading to TRAIL Resistance of Pancreatic Cancer Cell Lines" International Journal of Molecular Sciences 19, no. 6: 1661. https://doi.org/10.3390/ijms19061661