Exploration of Thiamin thiazole synthase (THI4) Expression and Transcriptomes Involved in the Floral Volatiles of Caladium bicolor
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Sample Preparation and Data Analyses for RNA-seq
2.3. Cloning and Transient Expression of Caladium THI4 Promoter
2.4. Analysis of a Transgene Expression Driven by Caladium THI4 Promoter
3. Results
3.1. Predicted Transcript Analyses
3.2. Putative THI4 Regulatory Transcripts
3.3. Male Tissue-Specific Expression of Caladium THI4 Promoter in Tobacco and Petunia Plants
4. Discussion
4.1. Transcripts Associated with Secondary Metabolite Biosynthesis Showing High Expression Coincided with De Novo C. bicolor Volatiles Emission
4.2. The Other Possible Transcripts Associated with the Flower Development of C. bicolor
4.3. CbTHI4 Promoter Is a Male Tissue-Specific
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene Names | Gene Description | Transcript Expression Increase (Counts) |
---|---|---|
HcPro | Polyprotein | 6496.03 |
MYB305 | Myb-related protein 305-like | 1918.39 |
ASG4/RVE3 | Transcription factor REVEILLE 3 | 1408.32 |
AG1 | Floral homeotic protein AGAMOUS | 764.23 |
NAC047 | NAC domain-containing protein | 718.10 |
ILL1 | IAA-amino acid hydrolase ILR1 | 602.33 |
MYB1R1 | Transcription factor MYB1R1 | 275.45 |
ZAT5 | Zinc finger protein | 274.07 |
NAC002 | NAC domain-containing protein 2 | 267.89 |
CAF1-11 | Putative CCR4-associated factor | 241.46 |
NAC48 | NAC domain-containing protein | 143.84 |
NAC21 | NAC domain-containing protein 21/22 | 121.13 |
NAC68 | NAC domain-containing protein 68 | 119.79 |
EREBP | EREBP-like factor | 105.17 |
MYBP | Transcription factor MYB, plant | 95.03 |
NAC21 | NAC domain-containing protein 21/22 | 91.09 |
EIN3 | Ethylene-insensitive protein 3 | 78.60 |
bHLH13 | Transcription factor | 73.69 |
bHLH146 | Transcription factor bHLH146-like | 66.49 |
RsfS | Ribosomal silencing factor RsfS | 62.47 |
Gene Names | Gene Description | Transcript Expression Decrease (Counts) |
---|---|---|
GRP1A | Glycine-rich RNA-binding protein | −1386.71 |
PABP2 | Polyadenylate-binding protein 2 | −568.98 |
ABCF1 | ABC transporter F family member 1 | −455.91 |
IAA27 | Auxin-responsive protein | −270.42 |
SPL13 | Squamosa promoter-binding-like protein 13 | −227.67 |
CPN60/groEL | Rubisco large subunit-binding protein beta, chloroplast-like | −218.24 |
SAHH1 | Adenosyl homocysteinase 1 | −188.89 |
CPN60 | Chaperonin CPN60-2, mitochondrial | −181.68 |
IAA27 | Auxin-responsive protein | −175.23 |
CPN60/groEL | Rubisco large subunit-binding protein beta, chloroplast-like | −172.44 |
HDT2 | Histone deacetylase HDT2 | −160.05 |
HDT2 | Histone deacetylase HDT2 | −126.55 |
ARF15 | Auxin response factor 15 | −106.02 |
WLIM1 | LIM domain-containing protein | −103.82 |
CPN60/groEL | Rubisco large subunit-binding protein beta, chloroplast-like | −93.12 |
RH15 | DEAD-box ATP-dependent RNA helicase 56 | −81.59 |
ZF-HD | Zinc-finger homeodomain protein | −76.05 |
AP3 | Floral homeotic protein APETALA 3 | −75.05 |
RNP1 | Heterogeneous nuclear ribonucleoprotein 1 | −72.78 |
ARF7 | Auxin response factor | −71.05 |
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Kim, J.Y.; Sigler, C.L.; Cho, K.H.; Gennaro, M.D.; Ellsworth, M.S.; Colquhoun, T.A. Exploration of Thiamin thiazole synthase (THI4) Expression and Transcriptomes Involved in the Floral Volatiles of Caladium bicolor. Horticulturae 2024, 10, 810. https://doi.org/10.3390/horticulturae10080810
Kim JY, Sigler CL, Cho KH, Gennaro MD, Ellsworth MS, Colquhoun TA. Exploration of Thiamin thiazole synthase (THI4) Expression and Transcriptomes Involved in the Floral Volatiles of Caladium bicolor. Horticulturae. 2024; 10(8):810. https://doi.org/10.3390/horticulturae10080810
Chicago/Turabian StyleKim, Joo Young, Cindy L. Sigler, Keun H. Cho, Madelyn D. Gennaro, Mara S. Ellsworth, and Thomas A. Colquhoun. 2024. "Exploration of Thiamin thiazole synthase (THI4) Expression and Transcriptomes Involved in the Floral Volatiles of Caladium bicolor" Horticulturae 10, no. 8: 810. https://doi.org/10.3390/horticulturae10080810