Genome-Wide Identification and Characterization of Sucrose Metabolism Genes Involved in Actinidia rufa and Their Expression Profiling during the Fruit Developmental Stages
Abstract
:1. Introduction
2. Materials and Methods
2.1. Identification of Sucrose Metabolism Genes in the A. rufa Genome
2.2. Phylogenetic Analysis
2.3. Gene Structure Construction, Conserved Motifs, and Cis-Acting Elements Identification
2.4. Subcellular Localization and Protein Physicochemical Properties Analysis
2.5. Collinearity and Selective Pressure Analysis
2.6. Transcriptome Profile Analysis
3. Results
3.1. Genome-Wide Identification of Sucrose Metabolism-Related Gene Families from A. rufa
3.2. Phylogenetic Analysis
3.3. Gene Structure, Conserved Motif, and Protein Domain Analysis
3.4. Promoter Cis-Elements Analysis of Sucrose Metabolism Genes
3.5. Collinearity and Selective Pressure Analysis of Sucrose Metabolism Genes
3.6. Genome-Wide Expression Analysis of Sucrose Metabolism Genes
3.7. GO and KEGG Functional Annotation of Sucrose Metabolism Genes
3.8. Temporal Gene Expression Dynamics during Fruit Development and Ripening
3.9. Identification of Key TFs Regulating Sucrose Metabolism
4. Discussion
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene Family | INV | SPP | SPS | SUS | SUC/SUT | SWEET | Total |
---|---|---|---|---|---|---|---|
Arabidopsis thaliana | 33 | 2 | 6 | 6 | 9 | 17 | 73 |
Actinidia chinensis | 31 | 3 | 6 | 10 | 10 | 29 | 89 |
Actinidia eriantha | 28 | 2 | 6 | 9 | 9 | 28 | 82 |
Actinidia hemsleyana | 29 | 2 | 6 | 7 | 7 | 19 | 70 |
Actinidia rufa | 31 | 2 | 4 | 9 | 6 | 10 | 62 |
Solanum lycopersicum | 19 | 2 | 4 | 6 | 2 | 25 | 58 |
Vitis vinifera | 21 | 5 | 7 | 7 | 5 | 20 | 65 |
Gene Name | Gene ID | Protein Length (aa) | Molecular Weight (kDa) | Isoelectric Point |
---|---|---|---|---|
ArINV1 | GFY84308.1 | 443 | 49.10 | 4.53 |
ArINV2 | GFY97846.1 | 533 | 59.94 | 6.35 |
ArINV3 | GFZ01189.1 | 867 | 96.97 | 5.23 |
ArINV4 | GFZ04274.1 | 452 | 50.42 | 6.56 |
ArINV5 | GFZ04278.1 | 456 | 51.59 | 9.22 |
ArINV6 | GFZ08509.1 | 507 | 57.58 | 6.37 |
ArINV7 | GFS29709.1 | 296 | 34.36 | 6.96 |
ArINV8 | GFS29713.1 | 379 | 42.65 | 5.4 |
ArINV9 | GFS39341.1 | 598 | 67.29 | 6.51 |
ArINV10 | GFS42808.1 | 570 | 64.96 | 5.88 |
ArINV11 | GFY85269.1 | 566 | 63.75 | 6.08 |
ArINV12 | GFY86245.1 | 570 | 65.27 | 6.24 |
ArINV13 | GFY89074.1 | 597 | 67.79 | 6.18 |
ArINV14 | GFY89880.1 | 574 | 65.60 | 6.02 |
ArINV15 | GFY90201.1 | 508 | 57.77 | 8.72 |
ArINV16 | GFY95297.1 | 552 | 63.07 | 6.31 |
ArINV17 | GFZ00601.1 | 576 | 65.73 | 6.08 |
ArINV18 | GFZ00609.1 | 615 | 70.02 | 6.24 |
ArINV19 | GFZ03044.1 | 191 | 21.67 | 5.24 |
ArINV20 | GFZ03045.1 | 106 | 11.80 | 5.57 |
ArINV21 | GFZ07245.1 | 645 | 73.37 | 5.53 |
ArINV22 | GFZ07303.1 | 571 | 64.00 | 5.35 |
ArINV23 | GFZ09104.1 | 466 | 52.63 | 5.74 |
ArINV24 | GFZ09106.1 | 540 | 61.00 | 6.55 |
ArINV25 | GFZ09762.1 | 105 | 12.04 | 9.36 |
ArINV26 | GFZ09763.1 | 382 | 43.82 | 5.71 |
ArINV27 | GFZ11195.1 | 654 | 73.46 | 5.88 |
ArINV28 | GFZ12668.1 | 618 | 68.82 | 6.09 |
ArINV29 | GFZ21641.1 | 668 | 75.08 | 6.31 |
ArINV30 | GFZ06978.1 | 652 | 73.02 | 4.99 |
ArINV31 | GFZ15206.1 | 514 | 58.10 | 6.64 |
ArSPP1 | GFY98003.1 | 395 | 44.70 | 5.6 |
ArSPP2 | GFZ09269.1 | 433 | 48.91 | 5.56 |
ArSPS1 | GFS40808.1 | 716 | 80.76 | 6.05 |
ArSPS2 | GFY88805.1 | 566 | 63.59 | 5.95 |
ArSPS3 | GFY95144.1 | 983 | 112.32 | 8.64 |
ArSPS4 | GFY99702.1 | 811 | 90.89 | 6.13 |
ArSUS1 | GFS35293.1 | 559 | 63.66 | 6.51 |
ArSUS2 | GFS35295.1 | 717 | 80.50 | 5.76 |
ArSUS3 | GFS35628.1 | 815 | 93.07 | 5.7 |
ArSUS4 | GFS35631.1 | 714 | 80.91 | 7.67 |
ArSUS5 | GFS37905.1 | 838 | 94.50 | 8.13 |
ArSUS6 | GFS38485.1 | 806 | 92.63 | 5.97 |
ArSUS7 | GFY98571.1 | 772 | 88.84 | 6.13 |
ArSUS8 | GFZ09599.1 | 1240 | 140.46 | 9.09 |
ArSUS9 | GFZ17994.1 | 819 | 93.16 | 8.07 |
ArSUT1 | GFS44113.1 | 452 | 49.42 | 9.48 |
ArSUT2 | GFY85479.1 | 493 | 53.53 | 9.28 |
ArSUT3 | GFY85509.1 | 518 | 54.68 | 9.17 |
ArSUT4 | GFY85510.1 | 499 | 53.00 | 9 |
ArSUT5 | GFY88434.1 | 591 | 63.84 | 6.8 |
ArSUT6 | GFY96916.1 | 504 | 53.61 | 8.94 |
ArSWEET1 | GFS37554.1 | 210 | 23.00 | 9.41 |
ArSWEET2 | GFS38071.1 | 280 | 30.70 | 7.76 |
ArSWEET3 | GFS38076.1 | 169 | 18.33 | 9.55 |
ArSWEET4 | GFS45098.1 | 69 | 7.68 | 8.01 |
ArSWEET5 | GFY89003.1 | 210 | 23.25 | 6.5 |
ArSWEET6 | GFY99936.1 | 231 | 25.82 | 6.95 |
ArSWEET7 | GFZ03923.1 | 247 | 27.40 | 9.37 |
ArSWEET8 | GFZ03924.1 | 220 | 25.07 | 8.58 |
ArSWEET9 | GFZ04631.1 | 195 | 22.06 | 5.6 |
ArSWEET10 | GFZ09897.1 | 252 | 27.56 | 9.71 |
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Jia, Y.; Qiang, X.; Dong, P.; Ren, T.; Zhang, Y.; Yang, Y. Genome-Wide Identification and Characterization of Sucrose Metabolism Genes Involved in Actinidia rufa and Their Expression Profiling during the Fruit Developmental Stages. Horticulturae 2024, 10, 772. https://doi.org/10.3390/horticulturae10070772
Jia Y, Qiang X, Dong P, Ren T, Zhang Y, Yang Y. Genome-Wide Identification and Characterization of Sucrose Metabolism Genes Involved in Actinidia rufa and Their Expression Profiling during the Fruit Developmental Stages. Horticulturae. 2024; 10(7):772. https://doi.org/10.3390/horticulturae10070772
Chicago/Turabian StyleJia, Yun, Xu Qiang, Pengbin Dong, Ting Ren, Ying Zhang, and Yanci Yang. 2024. "Genome-Wide Identification and Characterization of Sucrose Metabolism Genes Involved in Actinidia rufa and Their Expression Profiling during the Fruit Developmental Stages" Horticulturae 10, no. 7: 772. https://doi.org/10.3390/horticulturae10070772