Enterococcus faecalis Infection and Reactive Oxygen Species Down-Regulates the miR-17-92 Cluster in Gastric Adenocarcinoma Cell Culture
Abstract
:1. Introduction
2. Experimental
2.1. Cell Culture, E. faecalis, and Growth Conditions
2.2. Infection of Gastric Cells
2.3. Tert-Butyl Hydroperoxide Stimulation
2.4. RNA Isolation
2.5. Microarray
2.6. Quantitative Reverse Transcription PCR
2.7. Statistical Analysis
3. Results and Discussion
3.1. Living E. faecalis Affect Global miRNA Expression
3.2. E. faecalis Infection Alters the Expression of the miR-17-92 Cluster, miR-106-363 Cluster, and Other ROS Responsive miRNAs
3.3. TBHP Reduce the Expression of miRNAs from the miR-17-92 Cluster in a Time Dependent Manner
miRNA | Fold Change | Difference of Arbitrary Expression | p-Value | Corresponding miRNA Cluster |
---|---|---|---|---|
hsa-miR-17_st | −1.8 | 630.9 | 0.0001 | miR-17-92 cluster |
hsa-miR-18a_st | −9.9 | 245.7 | >0.0001 | |
hsa-miR-20a_st | −4.5 | 526.9 | >0.0001 | |
hsa-miR-19b_st | −4.1 | 271.2 | 0.0002 | miR-17-92 and miR-106-363 cluster |
hsa-miR-92a_st | 1.4 | −563.5 | 0.0023 | |
hsa-miR-18b_st | −15.8 | 33.4 | 0.0001 | miR-106-363 cluster |
hsa-miR-20b_st | −8.3 | 92.2 | >0.0001 | |
hsa-miR-106a_st | −1.9 | 580.9 | >0.0001 | |
hsa-miR-24-2-star_st | −3.6 | 53.4 | 0.0011 | ROS responsive miRs |
hsa-miR-27a-star_st | −14.7 | 54.0 | 0.0001 |
4. Conclusions
Supplementary Files
Supplementary File 1Acknowledgments
Author Contributions
Conflicts of Interest
References
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Strickertsson, J.A.B.; Rasmussen, L.J.; Friis-Hansen, L. Enterococcus faecalis Infection and Reactive Oxygen Species Down-Regulates the miR-17-92 Cluster in Gastric Adenocarcinoma Cell Culture. Genes 2014, 5, 726-738. https://doi.org/10.3390/genes5030726
Strickertsson JAB, Rasmussen LJ, Friis-Hansen L. Enterococcus faecalis Infection and Reactive Oxygen Species Down-Regulates the miR-17-92 Cluster in Gastric Adenocarcinoma Cell Culture. Genes. 2014; 5(3):726-738. https://doi.org/10.3390/genes5030726
Chicago/Turabian StyleStrickertsson, Jesper A. B., Lene Juel Rasmussen, and Lennart Friis-Hansen. 2014. "Enterococcus faecalis Infection and Reactive Oxygen Species Down-Regulates the miR-17-92 Cluster in Gastric Adenocarcinoma Cell Culture" Genes 5, no. 3: 726-738. https://doi.org/10.3390/genes5030726