Impact of Simulated Human Gastrointestinal Digestion on the Functional Properties of Dietary Fibres Obtained from Broccoli Leaves, Grape Stems, Pomegranate and Tomato Peels
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Dietary Fibre Extraction
2.2. Preparation of a Base Feed Supplemented with Different Types of Dietary Fibre
2.3. Faecal Inoculum
2.4. Human Simulated Digestion: Experimental Design
2.5. Characterisation of Digestion Extracts
2.5.1. Dietary Fibre Content
2.5.2. Dietary Fibre Composition
2.5.3. Non-Extractable Phenolic Compounds and Their Antioxidant Capacity
2.5.4. Microbial Population
2.5.5. SCFAs
2.5.6. Soluble Oligosaccharides
2.5.7. Antiproliferative Activity
2.6. Statistical Analysis
3. Results and Discussion
3.1. Content and Composition of the Dietary Fibre in Digestion Extracts
3.2. Functional Properties of Dietary Fibre
3.3. Oligosaccharides
3.4. Microbial Population Dynamics
3.5. SCFA Level during Simulated Digestion
3.6. Relationship of Fibre Composition, Functional Properties, SCFAs and Microbial Population to Digestion Stage and Fibre Type
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Stage | Conditions | Operation | ||||||
---|---|---|---|---|---|---|---|---|
Time | Vol (mL) | Temp (°C) | pH 1 | pO 2 | Stir (rpm) | Type | Vol (mL) | Description |
Digestion | ||||||||
0:00:00 | 600 | 20 | 6–6.5 | 21 | --- | Sampling | −100 | Initial sample |
0:10:00 | 0 | Transfer to | 500 | Stomach | ||||
Stomach (bioreactor 1) | ||||||||
0:00:00 | 750 | 37 | 2.5 | <0.5 | 150 | Initial content | 750 | PBS |
0:10:00 | 1350 | Transfer from | 500 | Batch | ||||
Supplementation | 100 | Pepsin 4.5% | ||||||
Sampling 1_1 | −25 | Microbial control | ||||||
3:00:00 | 1225 | Sampling 1_2 | −100 | Stomach sample | ||||
3:15:00 | 500 | Transfer to | 725 | Small intestine | ||||
Small intestine (bioreactor 2) | ||||||||
0:00:00 | 750 | 37 | 6.5 | <0.5 | 150 | Initial content | 750 | PBS |
3:15:00 | 1475 | Transfer from | 750 | Stomach | ||||
Supplementation | 100 | Pancreatin (3%); Bilis (7.5%) | ||||||
Sampling 2_1 | −25 | Microbial control | ||||||
7:00:00 | 6.5 | Start pH grad. 3 | ||||||
11:00:00 | 1450 | 7 | End pH grad. | |||||
Sampling 2_2 | −100 | Small intestine sample | ||||||
11:15:00 | 500 | Transfer to | 950 | Large gut | ||||
Colon (bioreactor 3) | ||||||||
0:00:00 | 750 | 37 | 5.7 | <0.5 | 150 | Initial content | 750 | PBS |
0:05:00 | 770 | Supplementation | 25 | MRS | ||||
0:10:00 | 790 | Supplementation | 15 | Faecal inoculum | ||||
11:00:00 | 750 | Sampling 3_1 | −40 | Microbial control | ||||
11:15:00 | 1700 | Transfer from | 1000 | Small intestine | ||||
5.7 | Start pH grad. | |||||||
24:00:00 | 1600 | Sampling 3_2 | −100 | Ascending sample | ||||
31:00:00 | 6 | pH grad. point | ||||||
48:00:00 | 1500 | Sampling 3_3 | −100 | Transverse sample | ||||
50:00:00 | 6.4 | pH grad. point | ||||||
70:00:00 | 6.8 | End pH grad. | ||||||
72:00:00 | 1400 | Sampling 3_4 | −100 | Descending sample |
Parameters 2 | Control | Dietary Fibre Type 1 | p-Values | ||||
---|---|---|---|---|---|---|---|
B_Leaf | P_Peel | G_Stem | T_Peel | ||||
Dietary fibre (g/kg) | 227 | 226 | 202 | 223 | 188 | 0.703 | |
Fiber constituents (mg/g dietary fibre) | |||||||
GalA | 578 | 588 | 686 | 669 | 526 | 0.121 | |
Glc | 34.3 b,3 | 27.2 ab | 35.5 b | 25.6 a | 34.2 b | 0.035 | |
Xyl/Gal/Man | <0.5 a | 2.12 b | 2.43 b | 2.21 b | <0.5 a | <0.001 | |
Rha/Ara | 3.3 | 2.3 | 2.4 | 1.9 | 2.8 | 0.310 | |
Fiber properties | |||||||
N-EPC (mg GAE/100 g) | 1.6 a | 3.7 a | 83.5 c | 15.7 b | 2.2 a | <0.001 | |
ABTS (mg Trolox/100 g) | 229 abc | 261 abc | 175 a | 266 bc | 216 ab | 0.025 | |
DPPH (mg Trolox/100 g) | 70 abc | 55 ab | 621 d | 95 bc | 23 a | <0.001 | |
Antiprol (% g inh) | 100.0 | 80.4 | 103.2 | 85.3 | 93.1 | 0.120 |
Parameters 2 | Control | Dietary Fibre 1 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|
B_Leaf 1 | P_Peel | G_Stem | T_Peel | |||||||
Stomach simulation | ||||||||||
Dietary Fibre (g/kg) | 172.7 b,3 | 127.6 a | 123.7 a | 163.9 b | 155.3 b | |||||
Fiber constituents (mg/g dietary fibre) | ||||||||||
GalA | 577.9 | 587.9 | 686.1 | 669.3 | 526.2 | |||||
Glc | 34.3 bc | 27.2 ab | 35.5 c | 25.6 a | 34.2 bc | |||||
Xyl/Gal/Man | <0.5 a | 2.1 b | 2.4 b | 2.2 b | <0.5 a | |||||
Rha/Ara | 3.3 | 2.3 | 2.4 | 1.9 | 2.8 | |||||
Fiber properties | ||||||||||
N-EPC (mg GAE/100 g) | 0.79 a | 1.99 a | 62.31 c | 12.89 b | 1.68 a | |||||
ABTS (mg Trolox/100 g) | 216 ab | 387 c | 933 d | 387 bc | 291 b | |||||
DPPH (mg Trolox/100 g) | 111.1 a | 62.7 a | 636.4 c | 235.1 b | 90.7 a | |||||
Antiprol (% g inh) | 105 | 80 | 105 | 88 | 92 | |||||
Small intestine simulation | ||||||||||
Fiber (g/kg) | 70.3 b | 58.8 a | 65.3 b | 85.6 c | 89.7 c | |||||
Fiber constituents (mg/g dietary fiber) | ||||||||||
GalA | 626.8 | 613.1 | 699.3 | 565.0 | 708.4 | |||||
Glc | 22.6 | 18.8 | 21.9 | 18.1 | 23.6 | |||||
Xyl/Gal/Man | 2.6 a | 3.9 ab | 4.0 b | 2.9 ab | 2.8 ab | |||||
Rha/Ara | 8.9 b | 4.9 a | 6.5 ab | 3.9 a | 5.5 ab | |||||
Fiber properties | ||||||||||
N-EPC (mg GAE/100 g) | 1.24 a | 1.23 a | 75.81 b | 6.25 a | 4.25 a | |||||
ABTS (mg Trolox/100 g) | 356 a | 310 a | 708 b | 351 a | 246 a | |||||
DPPH (mg Trolox/100 g) | 27.0 a | 3.2 a | 860.6 c | 149.0 b | 2.8 a | |||||
Antiprol (% g inh) | 99.0 b | 76.5 a | 55.6 a | 56.9 a | 82.2 b | Stage | ||||
Colon simulation | Prox | Trans | Distal | |||||||
Fiber (g/kg) | 59 b | 46 a | 59 b | 66 b | 76 c | 69 B,4 | 59 A | 56 A | ||
Fiber constituents (mg/g dietary fibre) | ||||||||||
GalA | 600.3 | 593.0 | 659.8 | 589.4 | 690.6 | 626.8 | 625.4 | 627.7 | ||
Glc | 20.8 c | 13.2 ab | 17.8 bc | 11.8 a | 26.6 d | 19.3 B | 19.1 AB | 15.8 A | ||
Xyl/Gal/Man | 2.4 a | 3.0 ab | 3.9 c | 2.5 ab | 3.2 b | 3.1 | 3.1 | 2.8 | ||
Rha/Ara | 8.5 d | 3.0 b | 5.7 c | 1.3 a | 7.0 cd | 5.4 B | 5.8 B | 4.0 A | ||
Fiber properties | ||||||||||
N-EPC (mg GAE/100 g) | 3.7 a | 2.3 a | 39.3 c | 6.3 b | 3.2 a | 11.1 B | 13.0 C | 8.8 A | ||
ABTS (mg Trolox/100 g) | 302 ab | 345 b | 732 d | 436 c | 274 a | 405 A | 447 B | 402 A | ||
DPPH (mg Trolox/100 g) | 20 a | 22 a | 534 c | 99 b | 16 a | 141 B | 160 B | 115 A | ||
Antiprol (% g inh) | 103 c | 80 ab | 89 abc | 77 a | 97 bc | 96 B | 80 A | 92 AB |
Oligosaccharides | Control | Dietary Fibre 1 | ||||
---|---|---|---|---|---|---|
B_Leaf | P_Peel | G_Stem | T_Peel | |||
Stomach simulation | ||||||
DP7 | 6.42 b,2 | 2.29 a | 23.60 e | 11.73 c | 19.99 d | |
DP6 | 0.04 | <0.01 | <0.01 | 0.03 | <0.01 | |
DP5 1 | <0.01 | 0.04 | 0.02 | 0.08 | 0.05 | |
DP5 2 | 0.10 | 0.04 | 0.08 | <0.01 | 0.06 | |
DP4 1 | 0.95 b | 0.56 a | 0.20 a | 0.92 b | 0.86 b | |
DP4 2 | <0.01 a | 0.67 b | 0.66 b | 1.16 d | 1.02 c | |
DP3 1 | 1.72 b | 1.01 a | 2.78 c | 1.05 a | 1.08 a | |
DP3 2 | 0.93 c | <0.01 a | <0.01 a | 0.85 b | <0.01 a | |
DP2 1 | 1.14 b | 1.07 a | 4.54 d | 1.76 c | 1.10 ab | |
DP2 2 | 1.60 b | 2.57 b | 6.83 b | <0.01 a | 2.07 b | |
DP2 3 | <0.01 a | <0.01 a | <0.01 a | <0.01 a | 4.64 b | |
Small intestine simulation | ||||||
DP7 | 18.59 b | 1.74 a | 25.50 d | 22.68 c | 22.14 c | |
DP6 | 0.28 b | <0.01 a | <0.01 a | 1.05 c | <0.01 a | |
DP5 1 | <0.01 a | <0.01 a | 0.59 b | 0.47 b | 1.18 c | |
DP5 2 | 0.79 d | 0.26 b | <0.01 a | <0.01 a | 0.67 c | |
DP4 1 | 0.52 d | <0.01 a | 0.26 c | 0.11 b | 0.22 c | |
DP4 2 | <0.01 a | 0.44 b | 0.54 b | 0.43 b | 0.69 c | |
DP3 1 | 1.17 c | 0.90 a | 2.73 d | 0.88 a | 1.07 b | |
DP3 2 | 0.90 b | <0.01 a | <0.01 a | 1.23 c | <0.01 a | |
DP2 1 | 1.84 c | <0.01 a | 3.37 d | 12.36 e | 1.07 b | |
DP2 2 | 13.06 b | <0.01 a | 19.28 d | <0.01 a | 17.37 c |
Microbial Population | Control | Dietary Fibre 1 | |||||||
---|---|---|---|---|---|---|---|---|---|
B_Leaf | P_Peel | G_Stem | T_Peel | ||||||
Small intestine simulation | |||||||||
Total viable bacteria | 8.91 b,2 | 9.18 c | 7.81 a | 9.42 d | 8.77 b | ||||
Enterobacteria | 8.54 b | 9.35 c | 7.81 a | 9.22 c | 9.00 c | ||||
Lactic acid bacteria | 5.18 b | <1 a | <1 a | <1 a | <1 a | ||||
Enterococci | 4.19 ab | 5.93 bc | 3.00 a | 7.39 c | 4.94 ab | ||||
Staphylococci | 4.95 a | 6.11 b | 5.76 b | 7.47 c | 7.14 c | ||||
Yeast | 1.2 a | 2.65 b | 2.93 b | 3.85 c | 3.33 c | Stage | |||
Colon simulation | Prox. | Trans. | Distal | ||||||
Total viable bacteria | 8.9 | 8.6 | 9.0 | 7.9 | 8.7 | 8.4 | 8.8 | 8.7 | |
Enterobacteria | 8.0 a | 8.4 b | 8.8 c | 8.7 c | 8.8 c | 8.6 B,3 | 8.6 B | 8.4 A | |
Lactic acid bacteria | 5.4 d | <1 a | 2.4 b | <1 a | 2.9 c | 3.2 C | 2.2 B | 1.1 A | |
Enterococci | 5.8 a | 6.4 b | 7.5 c | 5.8 a | 7.4 c | 6.7 | 6.5 | 6.5 | |
Staphylococci | 5.3 c | 3.9 b | 7.3 d | 3.6 a | 7.0 d | 6.0 B,3 | 6.4 C | 3.9 A | |
Bifidobacteria | 1.6 a | 5.1 c | 2.8 b | 5.4 d | 5.2 cd | 3.2 A | 4.9 C | 3.9 B | |
Yeast | 1.1 a | 2.2 b | 2.5 b | 3.5 c | 3.1 c | 3.2 C | 2.2 B | 1.1 A |
SCFAs | Control | Dietary Fibre 1 | |||||||
---|---|---|---|---|---|---|---|---|---|
B_Leaf | P_Peel | G_Stem | T_Peel | ||||||
Stomach simulation | |||||||||
Acetic acid | 191.08 c,2 | 20.44 a | 60.17 ab | 18.51 a | 99.36 b | ||||
Propionic acid | 34 b | 32 b | 33 b | 33 b | 24 a | ||||
Butyric acid | 5.49 c | 2.09 a | 3.74 b | 1.77 a | 6.42 c | ||||
Isovaleric acid | 0.63 b | 0.08 a | 0.19 a | 0.06 a | 0.05 a | ||||
Isobutyric acid | 0.03 | <0.01 | <0.01 | <0.01 | 0.08 | ||||
Isocaproic acid | <0.01 | <0.01 | <0.01 | <0.01 | 1.22 | ||||
Small intestine simulation | |||||||||
Acetic acid | 104.62 b | 36.83 a | 65.31 ab | 24.03 a | 50.10 a | ||||
Propionic acid | 60 | 59 | 60 | 60 | 51 | ||||
Butyric acid | 2.34 ab | 5.33 b | 3.30 ab | 1.80 a | 3.80 ab | ||||
Isovaleric acid | 0.31 | 0.02 | 0.13 | 0.14 | 0.07 | ||||
Isobutiric acid | 0.05 | 0.02 | 0.02 | <0.01 | 0.02 | ||||
Isocaproic acid | 0.06 | <0.01 | <0.01 | <0.01 | 0.02 | ||||
Valeric acid | 0.17 | 0.01 | 0.11 | 0.03 | 0.11 | Stage | |||
Colon simulation | Prox. | Trans. | Distal | ||||||
Acetic acid | 82.1 b | 27.6 a | 70.8 b | 17.6 a | 31.6 a | 47.7 | 44.7 | 45.4 | |
Propionic acid | 873 | 712 | 1033 | 1194 | 1354 | 1006 | 1033 | 1060 | |
Butyric acid | 13.0 c | 5.4 a | 10.4 bc | 3.8 a | 7.1 ab | 7.8 | 7.7 | 8.4 | |
Isovaleric acid | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | |
Isobutyric acid | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | |
Isocaproic acid | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | |
Caproic acid | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | |
Valeric acid | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 |
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Rivas, M.Á.; Ruiz-Moyano, S.; Vázquez-Hernández, M.; Benito, M.J.; Casquete, R.; Córdoba, M.d.G.; Martín, A. Impact of Simulated Human Gastrointestinal Digestion on the Functional Properties of Dietary Fibres Obtained from Broccoli Leaves, Grape Stems, Pomegranate and Tomato Peels. Foods 2024, 13, 2011. https://doi.org/10.3390/foods13132011
Rivas MÁ, Ruiz-Moyano S, Vázquez-Hernández M, Benito MJ, Casquete R, Córdoba MdG, Martín A. Impact of Simulated Human Gastrointestinal Digestion on the Functional Properties of Dietary Fibres Obtained from Broccoli Leaves, Grape Stems, Pomegranate and Tomato Peels. Foods. 2024; 13(13):2011. https://doi.org/10.3390/foods13132011
Chicago/Turabian StyleRivas, María Ángeles, Santiago Ruiz-Moyano, María Vázquez-Hernández, María José Benito, Rocío Casquete, María de Guía Córdoba, and Alberto Martín. 2024. "Impact of Simulated Human Gastrointestinal Digestion on the Functional Properties of Dietary Fibres Obtained from Broccoli Leaves, Grape Stems, Pomegranate and Tomato Peels" Foods 13, no. 13: 2011. https://doi.org/10.3390/foods13132011