News from the Sea: A New Genus and Seven New Species in the Pleosporalean Families Roussoellaceae and Thyridariaceae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Isolates
2.2. Morphological Analysis
2.3. DNA Extraction, PCR Amplification, and Data Assembling
2.4. Sequence Alignment and Phylogenetic Analysis
3. Results
3.1. Phylogenetic Inference
3.2. Taxonomy
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | Strain Code | Source | nrITS | nrSSU | nrLSU | TEF-1α | RPB2 |
---|---|---|---|---|---|---|---|
Roussoellaceae | |||||||
Arthopyrenia salicis Massal | CBS 368.94 | Salix bark | KF443410 | AY538333 | AY538339 | KF443404 | KF443397 |
Neoroussoella bambusae Liu and Hyde | MFLUCC 11-0124 | Dead branch of Bambusa | KJ474827 | -- | KJ474839 | KJ474848 | KJ474856 |
N. alishanense Karunarathna, Kuo, Phookamsak and Hyde | AKTW 03 FU31016 | Pennisetum purpureum | MK503816 | MK503828 | MK503822 | MK336181 | MN037756 |
AKTW 11 FU31018 | Pennisetum purpureum | MK503818 | MK503830 | MK503824 | MK336182 | MN037757 | |
N. entadae Jones and Hyde | MFLUCC 18-0243 | Leucaena sp. | MK347786 | MK347893 | MK348004 | MK360065 | MK434866 |
N. heveae Senwanna, Phookamsak and Hyde | MFLUCC 17-1983 | Twig of Hevea brasiliensis | MH590693 | -- | MH590689 | -- | -- |
N. leucaenae Jones and Hyde | MFLUCC 18-1544 | Decaying pod of Leucaena | MK347767 | MK347874 | MK347984 | MK360067 | MK434876 |
MFLUCC 17-0927 | Pterocarpus sp. | MK347733 | MK347841 | MK347950 | MK360066 | MK434896 | |
Neoroussoella lignicolasp. nov. | MUT 4904 | P. pavonica | KT699129 | MN556307* | MN556319* | MN605894* | MN605914* |
MUT 5008 | P. oceanica leaves | MN556317* | MN556308* | MN556320* | MN605895* | MN605915* | |
MUT 5373 | P. pavonica | KU314953 | KU314954 | MN556321* | MN605896* | MN605916* | |
Pararoussoella juglandicola Crous and Schumach | CBS 145037 | MK442607 | -- | MK442543 | MK442699 | MK442671 | |
P. mukdahanensis (Phookamsak, Dai and Hyde) Crous | MFLUCC 11-0201 | Bamboo | KU940129 | KU872121 | KU863118 | -- | -- |
P. rosarum Jones and Hyde | MFLUCC 17-0796 | Rosa sp. | MG8289391 | MG829154 | MG829048 | MG829224 | -- |
Pseudoneoconiothyrium rosae (Phukhams., Camporesi and Hyde) Phukhams., Camporesi and Hyde | MFLUCC 15-0052 | Dead aerial spines of Rosa canina | MG828922 | MG829138 | MG829032 | -- | -- |
Roussoella chiangraina Phookamsak, Liu and Hyde | MFLUCC 10-0556 | Dead branch of bamboo | KJ474828 | -- | KJ474840 | KJ474849 | KJ474857 |
R. doimaesalongensis Thambug. and Hyde | MFLUCC 14–0584 | Dead branch of bamboo | KY026584 | -- | KY000659 | KY651249 | KY678394 |
R. elaeicola Konta. and Hyde | MFLUCC 15-0276a | Dead petiole of Elaeis guineensis | MH742329 | -- | MH742326 | -- | -- |
MFLUCC 15-0276b | Dead petiole of Elaeis guineensis | MH742330 | -- | MH742327 | -- | -- | |
R. euonymi Crous and Akulov | CBS 143426 | Fallen branches of Euonymus europaeus | MH107915 | -- | MH107961 | -- | MH108007 |
R. hysterioides (Ces.) Höhn. | CBS 546.94 | Phyllostachys | KF443405 | AY642528 | KF443381 | KF443399 | KF443392 |
R. intermedia Ju, Rogers and Huhndorf | CBS 170.96 | Bamboo | KF443407 | KF443390 | KF443382 | KF443398 | KF443394 |
R. japanensisKaz. Tanaka, Liu and Hyde | MAFF 239636 | Twigs of Sasa veitchii | KJ474829 | AB524480 | AB524621 | AB539114 | AB539101 |
R. kunmingensis Jiang, Phookamsak and Hyde | KUMCC 18-0128 | Dead bamboo | MH453491 | -- | MH453487 | MH453480 | MH453484 |
R. mangrovei Phukhams. and Hyde | MFLUCC 16-0424 | Dead branches of Rhizophora | MH025951 | -- | MH023318 | MH028246 | MH028250 |
Roussoella margidorensis sp. nov. | MUT 5329 | P. pavonica | KU314944 | MN556309* | MN556322* | MN605897* | MN605917* |
Roussoella mediterranea sp. nov. | MUT 5306 | P. pavonica | KU255054 | MN556310* | MN556323* | MN605898* | MN605918* |
MUT 5369 | P. pavonica | KU314947 | KU314948 | MN556324* | MN605899* | MN605919* | |
R. mexicana Crous and Yáñez-Mor. | CPC 25355 | Leaf spots of Coffea arabica | KT950848 | -- | KT950862 | -- | -- |
R. neopustulans Dai, Liu and Hyde | MFLUCC 11-0609 | Bamboo | KJ474833 | -- | KJ474841 | KJ474850 | |
MFLUCC 12-0003 | Bamboo | KU940130 | KU872122 | KU863119 | -- | -- | |
R. nitidula Sacc. and Paol. | MFLUCC 11-0182 | Bamboo | KJ474835 | -- | KJ474843 | KJ474852 | KJ474859 |
MFLUCC 11-0634 | Bamboo | KJ474834 | -- | KJ474842 | KJ474851 | KJ474858 | |
Roussoella padinae sp. nov. | MUT 5341 | P. pavonica | KU158153 | KU158176 | MN556325* | MN605900* | MN605920* |
MUT 5365 | P. pavonica | KU158170 | KU158179 | MN556326* | MN605901* | MN605921* | |
MUT 5503 | P. pavonica | KU158170 | MN556312* | MN556327* | MN605902* | MN605922* | |
R. pseudohysterioides Dai and Hyde | MFLUCC 13-0852 | Bamboo | KU940131 | KU872123 | KU863120 | KU940198 | -- |
R. pustulans (Ellis and Everh.) Ju, Rogers and Huhndorf | KT 1709 | Culms of Sasa kurilensis | KJ474830 | AB524482 | AB524623 | AB539116 | AB539103 |
R. scabrispora (Höhn.) Aptroot | MFLUCC 11-0624 | Bamboo | KJ474836 | -- | KJ474844 | KJ474853 | KJ474860 |
RSC | Bamboo | KX650566 | -- | KX650566 | KX650537 | -- | |
R. siamensis Phookamsak, Liu and Hyde | MFLUCC 11-0149 | Bamboo | KJ474837 | KU872125 | KJ474845 | KJ474854 | KJ474861 |
R. thailandica Dai, Liu and Hyde | MFLUCC 11-0621 | Bamboo | KJ474838 | -- | KJ474846 | -- | |
R. tuberculata Dai and Hyde | MFLUCC 13-0854 | Bamboo | KU940132 | KU872124 | KU863121 | KU940199 | |
R. verrucispora Kaz. Tanaka, Liu and Hyde | CBS 125434 | Sasa kurilensis | KJ474832 | AB52448 | AB524622 | AB539115 | -- |
R. yunnanensis Jiang, Phookamsak and Hyde | KUMCC 18-0115 | Dead bamboo | MH453492 | -- | MH453488 | MH453481 | -- |
Roussoellopsis macrospora (Hino and Katum.) Hinoand Katum | MFLUCC 12-0005 | Bamboo | KJ739604 | KJ739608 | KJ474847 | KJ474855 | KJ474862 |
Ro. tosaensis (Hino and Katum.) Hino and Katum | KT 1659 | Culms of bamboo | -- | AB524484 | AB524625 | AB539117 | AB539104 |
Thyridariaceae | |||||||
Cycasicola goaensis Jones and Hyde | MFLUCC 17-0754 | Cycas sp. | MG828885 | MG829112 | MG829001 | MG829198 | -- |
C. leucaenae Jones and Hyde | MFLUCC 17-0914 | Leucaena leucocephala | MK34772 | MK347833 | MK347942 | MK360046 | MK434900 |
Liua muriformis Phookamsak, Jiang and Hyde | KUMCC 18-0177 | Dead hanging branches of Lonicera maackii | MK433599 | MK433595 | MK433598 | MK426798 | MK426799 |
Parathyridaria percutanea (Ahmed, Stevens, van de Sande and de Hoog) Jaklitsch and Voglmayr | CBS 868.95 | Human | KF322118 | KF366451 | KF366449 | KF407987 | KF366452 |
CBS 128203 | Human | KF322117 | KF366450 | KF366448 | KF407988 | KF366453 | |
P. ramulicola Jaklitsch, Fourn and Voglmayr | CBS 141479 | Twigs of Ribes rubrum | NR_147657 | KX650514 | KX650565 | KX650536 | KX650584 |
MUT 4397 | P. oceanica | KC339235 | MN556311* | KF636775 | MN605913* | MN605933* | |
P. robiniaeMapook, Camporesi and Hyde | MFLUCC 14-1119 | Dead branch of Robinia pseudoacacia | KY511142 | -- | KY511141 | KY549682 | -- |
MUT 2452 | Dysidea fragilis | MG813183 | MN556312* | MG816491 | MN605903* | MN605923* | |
MUT 4893 | P. pavonica | KM355998 | KM355993 | MN556328* | MN605904* | MN605924* | |
Parathyridaria flabelliae sp. nov. | MUT 4859 | F. petiolata | KR014355 | KT587315 | KP671716 | MN605909* | MN605929* |
MUT 4886 | F. petiolata | KR014358 | KT587317 | KP671720 | MN605910* | MN605930* | |
Parathyridaria tyrrhenica sp. nov. | MUT 4966 | F. petiolata, | KR014366 | KT587309 | KP671740 | MN605911* | MN605931* |
MUT 5371 | P. pavonica | KU314951 | KU314952 | MN556329* | MN605912* | MN605932* | |
Parathyridariella dematiacea sp. nov. | MUT 4419 | P. oceanica rhizomes | KC339245 | MN556313* | KF636786 | MN605905* | MN605925* |
MUT 4884 | F. petiolata | MN556317* | KT587329 | KP671726 | MN605906* | MN605926* | |
MUT 5310 | P. pavonica | KU255057 | MN556314* | MN556330* | MN605907* | MN605927* | |
MUT 5381 | P. pavonica | KU314959 | KU314960 | MN556331* | MN605908* | MN605928* | |
Thyridaria acaciae (Crous and Wingf.) Jaklitsch and Voglmayr | CBS 138873 | Leaves of Acacia tortilis | KP004469 | -- | KP004497 | -- | -- |
T. broussonetiae (Sacc.) Traverso | TB | Hippocrepis emerus | KX650567 | -- | KX650567 | KX650538 | KX650585 |
TB1 | Amorpha fruticosa | KX650568 | KX650515 | KX650568 | KX650539 | KX650586 | |
Thyridariella mahakoshae Devadatha, Sarma, Wanas., Hyde and Jones | NFCCI 4215 | Decaying wood Avicennia marina | MG020435 | MG020441 | MG020438 | MG023140 | MG020446 |
Th. mangrovei Devadatha, Sarma, Hyde, Wanas. and Jones | NFCCI 4213 | Decaying wood Avicennia marina | MG020434 | MG020440 | MG020437 | MG020443 | MG020445 |
NFCCI 4214 | Decaying wood Avicennia marina | MG020436 | MG020442 | MG020439 | MG020444 | MG020447 | |
Occultibambusaceae | |||||||
Occultibambusa bambusae Dai and Hyde | MFLUCC 11-0394 | Bamboo | KU940124 | -- | KU863113 | KU940194 | KU940171 |
MFLUCC 13-0855 | Bamboo | KU940123 | KU872116 | KU863112 | KU940193 | KU940170 | |
Ohleriaceae | |||||||
Ohleria modesta Fuckel | MGC | KX650562 | -- | KX650562 | KX650533 | KX650582 | |
OM | Branches of Chamaecytisus proliferus | KX650563 | KX650513 | KX650563 | KX650534 | KX650583 | |
Torulaceae | |||||||
Dendryphion europaeum Crous and Schumacher | CPC 22943 | Heracleum sphondylium | KJ869146 | -- | KJ869203 | -- | -- |
Torula herbarum (Pers.) Link | CBS 111855 | n.a. | KF443409 | KF443391 | KF443386 | KF443403 | KF443396 |
CBS 595.96 | KF443408 | KF443387 | KF443385 | KF443402 | KF443395 | ||
Torula hollandica Crous | CBS 220.69 | Delphinium dead stem | KF443406 | -- | MH877717 | -- | -- |
Forward and Reverse Primers | Thermocycler Conditions | References | |
---|---|---|---|
ITS | ITS1- ITS4 | 95 °C: 5 min, (95 °C: 40 s, 55 °C: 50 s, 72 °C: 50 sec) × 35 cycles; 72 °C: 8 min; 4 °C: ∞ | [23] |
LSU | LR0R-LR7 | 95 °C: 5 min, (95 °C: 1 min, 50 °C: 1 min, 72 °C: 2 min) × 35 cycles; 72 °C: 10 min; 4 °C: ∞ | [24] |
SSU | NS1-NS4 | 95 °C: 5 min, (95 °C: 1 min, 50 °C: 1 min, 72 °C: 2 min) × 35 cycles; 72 °C: 10 min; 4 °C: ∞ | [23] |
TEF-1α | 1018F/1620R | 95 °C: 5 min, (95 °C: 1 min, 50 °C: 1 min; 72 °C: 2 min) × 40 cycles, 72 °C: 10 min; 4 °C: ∞ | [25] |
RPB2 | fRPB2-5F/fPB2-7cR | 94 °C: 3 min, (94 °C: 30 s; 55 °C: 30 s; 72 °C: 1 min) × 40 cycles, 72 °C: 10 min; 4 °C: ∞ | [26] |
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Poli, A.; Bovio, E.; Ranieri, L.; Varese, G.C.; Prigione, V. News from the Sea: A New Genus and Seven New Species in the Pleosporalean Families Roussoellaceae and Thyridariaceae. Diversity 2020, 12, 144. https://doi.org/10.3390/d12040144
Poli A, Bovio E, Ranieri L, Varese GC, Prigione V. News from the Sea: A New Genus and Seven New Species in the Pleosporalean Families Roussoellaceae and Thyridariaceae. Diversity. 2020; 12(4):144. https://doi.org/10.3390/d12040144
Chicago/Turabian StylePoli, Anna, Elena Bovio, Lucrezia Ranieri, Giovanna Cristina Varese, and Valeria Prigione. 2020. "News from the Sea: A New Genus and Seven New Species in the Pleosporalean Families Roussoellaceae and Thyridariaceae" Diversity 12, no. 4: 144. https://doi.org/10.3390/d12040144