A YAP/TAZ–ARHGAP29–RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions
Abstract
:1. Introduction
2. Methods
2.1. Nephrocyte Analysis
2.2. Cell Culture
2.3. Expression Plasmids
2.4. Antibodies
2.5. Western Blot Analysis
2.6. Analysis of Active RhoA
2.7. Immunofluorescence, Cell Spreading, and Integrin Adhesion Complex Analysis
2.8. Glomerular Disease Analysis
2.9. GlomAssay
2.10. RNA Sequencing
2.11. Proteomics
2.12. Statistical Analysis
3. Results
3.1. Loss of EPB41L5 Impairs YAP/TAZ-TEAD Complex Mediated Transcriptional Signaling
3.2. Inhibition of YAP/TAZ-TEAD Complex Mediated Transcription Impairs Formation of Cell Protrusions, IACs and Expression of Podocyte Genes
3.3. ARHGAP29 Limits IAC Maturation and Promotes Cell Protrusion Formation
3.4. ARHGAP29 Expression Is Induced as a Consequence of Mild Podocyte Damage
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Rogg, M.; Maier, J.I.; Helmstädter, M.; Sammarco, A.; Kliewe, F.; Kretz, O.; Weißer, L.; Van Wymersch, C.; Findeisen, K.; Koessinger, A.L.; et al. A YAP/TAZ–ARHGAP29–RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions. Cells 2023, 12, 1795. https://doi.org/10.3390/cells12131795
Rogg M, Maier JI, Helmstädter M, Sammarco A, Kliewe F, Kretz O, Weißer L, Van Wymersch C, Findeisen K, Koessinger AL, et al. A YAP/TAZ–ARHGAP29–RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions. Cells. 2023; 12(13):1795. https://doi.org/10.3390/cells12131795
Chicago/Turabian StyleRogg, Manuel, Jasmin I. Maier, Martin Helmstädter, Alena Sammarco, Felix Kliewe, Oliver Kretz, Lisa Weißer, Clara Van Wymersch, Karla Findeisen, Anna L. Koessinger, and et al. 2023. "A YAP/TAZ–ARHGAP29–RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions" Cells 12, no. 13: 1795. https://doi.org/10.3390/cells12131795