Proteomics and Metabolomics Profiling of Platelets and Plasma Mediators of Thrombo-Inflammation in Gestational Hypertension and Preeclampsia
Abstract
:1. Introduction
2. Methods
2.1. Methods Statement
2.2. Human Platelet-Rich Plasma Preparation
2.3. Plasma and Platelet Lysates Preparation
2.4. Quantitative Proteomics
2.5. High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS)
2.6. Proteomic Data and Bioinformatics Analysis
2.7. Protein-Protein Interaction Analysis
2.8. Metabolomics
2.9. Metabolomic Analysis
2.10. Multiplex Analysis of Human Cytokines
2.11. Platelet Procoagulant Membrane Dynamics Imaging
2.12. Statistical Analysis
2.13. Data and Code Availability
3. Results
3.1. Study Participants
3.2. Plasma Proteomics Analysis
3.3. Multiplex Immunoassays
3.4. Platelets Proteomics Analysis
3.5. Recombinant Human S100A8/S100A9 Heterodimer Induced Platelet P-Selectin Membrane Expression, Integrin Activation and Clumping In Vitro
3.6. Protein-Protein Interaction Analysis
3.7. Metabolomics Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Non-Pregnant (n = 4) (NP) | Healthy Pregnant (n = 5) (PC) | Gestational Hypertension (n = 4) (GH) | Preeclamptic Pregnancy (n = 4) (PE) | |
---|---|---|---|---|
Demographics | ||||
Age (years) | 35.0 ± 6.5 | 32.0 ± 7.1 | 35.0 ± 5.9 | 32.0 ± 6.1 |
Gestational Age (days) | 275.4 ± 4.5 | 250.8 ± 26.5 | 219.6 ± 39.7 | |
Height (cm) | 162.0 ± 3.7 | 164.4 ± 2.8 | 162 ± 4.8 | 161.3 ± 9.8 |
Weight (kg) | 55.8 ± 3.8 | 73.4 ± 9.3 | 84.2 ± 6.3 | 77.4 ± 10.3 |
BMI (kg/m2) | 21.2 ± 1.6 | 27.9 ± 3.4 | 32.2 ± 4.2 | 29.9 ± 4.7 |
Urine Protein (g/L) | Not Collected | Not Collected | 0.11 ± 0.04 | 0.60 ± 0.85 |
sBP (mmHg) * | 105 ± 5 | 112 ± 11 | 143 ± 2 | 149 ± 10 |
dBP (mmHg) * | 5 ± 4 | 67 ± 9 | 85 ± 7 | 90 ± 3 |
Hemoglobin levels (g/L) | 133.3 ± 12.0 | 133.2 ± 7.3 | 120.2 ± 6.2 | 123.4 ± 11.1 |
Positive GDM Screen ** | N/A | 0 | 0 | 0 |
Platelet Indices | ||||
Platelet count (109/L) | 270.3 ± 63.6 | 186.0 ± 44.6 | 206.8 ± 33.0 | 252.0 ± 65.9 |
Mean Platelet Volume (fL) | 10.0 ± 0.8 | 9.1 ± 4.1 | 12.2 ± 0.9 | 11.7 ± 0.7 |
Immature Platelet Fraction (%) | 2.4 ± 1.2 | 5.0 ± 3.0 | 9.5 ± 3.5 | 7.8 ± 3.1 |
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de Almeida, L.G.N.; Young, D.; Chow, L.; Nicholas, J.; Lee, A.; Poon, M.-C.; Dufour, A.; Agbani, E.O. Proteomics and Metabolomics Profiling of Platelets and Plasma Mediators of Thrombo-Inflammation in Gestational Hypertension and Preeclampsia. Cells 2022, 11, 1256. https://doi.org/10.3390/cells11081256
de Almeida LGN, Young D, Chow L, Nicholas J, Lee A, Poon M-C, Dufour A, Agbani EO. Proteomics and Metabolomics Profiling of Platelets and Plasma Mediators of Thrombo-Inflammation in Gestational Hypertension and Preeclampsia. Cells. 2022; 11(8):1256. https://doi.org/10.3390/cells11081256
Chicago/Turabian Stylede Almeida, Luiz Gustavo N., Daniel Young, Lorraine Chow, Joshua Nicholas, Adrienne Lee, Man-Chiu Poon, Antoine Dufour, and Ejaife O. Agbani. 2022. "Proteomics and Metabolomics Profiling of Platelets and Plasma Mediators of Thrombo-Inflammation in Gestational Hypertension and Preeclampsia" Cells 11, no. 8: 1256. https://doi.org/10.3390/cells11081256