Enhanced Specificity in Loop-Mediated Isothermal Amplification with Poly(ethylene glycol)-Engrafted Graphene Oxide for Detection of Viral Genes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemical Reagents and DNA Polymerase
2.2. Primer DNAs
2.3. Total RNA Extraction and Reverse Transcription
2.4. Preparation of PEG-nGO
2.5. LAMP Assays
2.6. Gel Electrophoresis
2.7. ssDNA Adsorption on PEG-nGO
2.8. RT-qPCR
3. Results and Discussion
3.1. Working Mechanism of LAMP Facilitation by PEG-nGO
3.2. Specificity and Sensitivity of PEG-nGO-Based LAMP
3.3. Detection of HCV-Positive and HCV-Negative Serum Samples by PEG-nGO-Based LAMP
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ku, J.; Chauhan, K.; Hwang, S.-H.; Jeong, Y.-J.; Kim, D.-E. Enhanced Specificity in Loop-Mediated Isothermal Amplification with Poly(ethylene glycol)-Engrafted Graphene Oxide for Detection of Viral Genes. Biosensors 2022, 12, 661. https://doi.org/10.3390/bios12080661
Ku J, Chauhan K, Hwang S-H, Jeong Y-J, Kim D-E. Enhanced Specificity in Loop-Mediated Isothermal Amplification with Poly(ethylene glycol)-Engrafted Graphene Oxide for Detection of Viral Genes. Biosensors. 2022; 12(8):661. https://doi.org/10.3390/bios12080661
Chicago/Turabian StyleKu, Jamin, Khushbu Chauhan, Sang-Hyun Hwang, Yong-Joo Jeong, and Dong-Eun Kim. 2022. "Enhanced Specificity in Loop-Mediated Isothermal Amplification with Poly(ethylene glycol)-Engrafted Graphene Oxide for Detection of Viral Genes" Biosensors 12, no. 8: 661. https://doi.org/10.3390/bios12080661