AG-9, an Elastin-Derived Peptide, Increases In Vitro Oral Tongue Carcinoma Cell Invasion, through an Increase in MMP-2 Secretion and MT1-MMP Expression, in a RPSA-Dependent Manner
Abstract
:1. Introduction
2. Materials and Methods
2.1. Peptide Synthesis
2.2. Cell Culture
2.3. Cytotoxicity Assay
2.4. Proliferation Assay
2.5. In Vitro Invasion Assays
2.6. Zymography Analyses
2.6.1. Cell Incubation with Effectors
2.6.2. Gelatin Zymography
2.6.3. Gelatin-Plasminogen Zymography
2.7. Western-Blot Analyses
2.8. Immunocytochemistry
2.9. RNA Isolation and Real-Time PCR Analysis
2.10. SiRNA Transfection
2.11. Docking Experiments
2.12. Statistical Analysis
3. Results
3.1. CAL 27 Cells Express RPSA Receptor
3.2. AG-9 Increases CAL 27 Invasion through Matrigel®, MMP-2 Secretion, and MT1-MMP Expression
3.3. AG-9 Proinvasive Effects Are Mediated through RPSA Receptor
3.4. In Silico Study of AG-9 and EGCG Binding on RPSA
3.5. In Vitro EGCG Cytotoxicity on CAL 27 Cells
3.6. EGCG Prevents AG-9 Stimulatory Effect on CAL 27 Migration, Invasion, MMP-2 Secretion, and MT1-MMP Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bretaudeau, C.; Baud, S.; Dupont-Deshorgue, A.; Cousin, R.; Brassart, B.; Brassart-Pasco, S. AG-9, an Elastin-Derived Peptide, Increases In Vitro Oral Tongue Carcinoma Cell Invasion, through an Increase in MMP-2 Secretion and MT1-MMP Expression, in a RPSA-Dependent Manner. Biomolecules 2021, 11, 39. https://doi.org/10.3390/biom11010039
Bretaudeau C, Baud S, Dupont-Deshorgue A, Cousin R, Brassart B, Brassart-Pasco S. AG-9, an Elastin-Derived Peptide, Increases In Vitro Oral Tongue Carcinoma Cell Invasion, through an Increase in MMP-2 Secretion and MT1-MMP Expression, in a RPSA-Dependent Manner. Biomolecules. 2021; 11(1):39. https://doi.org/10.3390/biom11010039
Chicago/Turabian StyleBretaudeau, Clara, Stéphanie Baud, Aurélie Dupont-Deshorgue, Rémi Cousin, Bertrand Brassart, and Sylvie Brassart-Pasco. 2021. "AG-9, an Elastin-Derived Peptide, Increases In Vitro Oral Tongue Carcinoma Cell Invasion, through an Increase in MMP-2 Secretion and MT1-MMP Expression, in a RPSA-Dependent Manner" Biomolecules 11, no. 1: 39. https://doi.org/10.3390/biom11010039