Natural Compounds Modulate Drug Transporter Mediated Oral Cancer Treatment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Lines and Culture
2.2. ALA-PDT
2.3. 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) Assay
2.4. Western Blot Analysis
2.5. PpIX Detection
2.6. Primary Tumor Sphere Culture
2.7. ABCG2 Detection
2.8. Aldehyde dehydrogenase (ALDH) Assay
2.9. Statistical Analysis
3. Results
3.1. PpIX Accumulation Inversely Correlates with ABCG2 Expression
3.2. Gefitinib, Curcumin, and EGCG Inhibited ABCG2 Expression and Modulated PpIX Accumulation and ALA-PDT Efficiency
3.3. ABCG2 Expression Correlated with Stemness, Tumor Grade, and Clinical Prognosis of HNCs
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Yang, H.; Wei, Y.-C.; Li, W.-C.; Chen, H.-Y.; Lin, H.-Y.; Chiang, C.-P.; Chen, H.-M. Natural Compounds Modulate Drug Transporter Mediated Oral Cancer Treatment. Biomolecules 2020, 10, 1335. https://doi.org/10.3390/biom10091335
Yang H, Wei Y-C, Li W-C, Chen H-Y, Lin H-Y, Chiang C-P, Chen H-M. Natural Compounds Modulate Drug Transporter Mediated Oral Cancer Treatment. Biomolecules. 2020; 10(9):1335. https://doi.org/10.3390/biom10091335
Chicago/Turabian StyleYang, Hsiang, Yu-Ching Wei, Wan-Chun Li, Hsin-Yung Chen, Hung-Ying Lin, Chun-Pin Chiang, and Hsin-Ming Chen. 2020. "Natural Compounds Modulate Drug Transporter Mediated Oral Cancer Treatment" Biomolecules 10, no. 9: 1335. https://doi.org/10.3390/biom10091335