PRF Lysates Modulate Chemokine Expression in Oral Squamous Carcinoma and Healthy Epithelial Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Preparation of Solid PRF Lysates
2.3. Reverse Transcription Quantitative Real-Time PCR (RT-qPCR) and Immunoassay
2.4. Immunofluorescence Analysis
2.5. Statistical Analysis
3. Results
3.1. PRF Lysates Suppress IL1β- and TNFα-Induced Chemokine Expression in HSC2 Cells
3.2. PRF Lysates Suppress Induced Chemokine Expression in TR146 Cells
3.3. Solid PRF Lysates Reduced the Cytokine-Induced Nuclear Translocation of p65 in HSC2 Cells
3.4. Solid PRF Lysates Suppress Poly(I:C) HMW-Induced Chemokine Expression in HSC2 Cells
3.5. Liquid PRF Failed to Suppress Cytokine-Induced Chemokine Expression in HSC2 Cells
3.6. PRF Lysates Increased CXCL8, CXCL1, and CXCL2 Expression in Oral Epithelial Cells
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Afradi, Z.; Panahipour, L.; Abbas Zadeh, S.; Gruber, R. PRF Lysates Modulate Chemokine Expression in Oral Squamous Carcinoma and Healthy Epithelial Cells. Bioengineering 2024, 11, 746. https://doi.org/10.3390/bioengineering11080746
Afradi Z, Panahipour L, Abbas Zadeh S, Gruber R. PRF Lysates Modulate Chemokine Expression in Oral Squamous Carcinoma and Healthy Epithelial Cells. Bioengineering. 2024; 11(8):746. https://doi.org/10.3390/bioengineering11080746
Chicago/Turabian StyleAfradi, Zohreh, Layla Panahipour, Salman Abbas Zadeh, and Reinhard Gruber. 2024. "PRF Lysates Modulate Chemokine Expression in Oral Squamous Carcinoma and Healthy Epithelial Cells" Bioengineering 11, no. 8: 746. https://doi.org/10.3390/bioengineering11080746