Cold Argon Plasma as Adjuvant Tumour Therapy on Progressive Head and Neck Cancer: A Preclinical Study
Abstract
:1. Introduction
2. Methods
2.1. Cell Culture
2.2. Tissue Samples
2.3. Plasma Source and Plasma Treatment
2.4. Cell Viability
2.5. Cell Cycle Analyses
2.6. Cell Motility Assay
2.7. DNA Fragmentation
2.8. Cytochrome C Measurements
2.9. Cytokine Detection
2.10. Global Protein Expression
2.11. Statistics
3. Results
3.1. Examinations on Cultured Cells
3.1.1. CAP Reduced Viability in Normal and Tumour Cells
3.1.2. Cell Cycle Arrest after CAP Treatment in HaCaT Keratinocytes and in HNO97 Cells
3.1.3. CAP Exposure Activated Caspase in HNO97 Cancer Cells and HaCaT Keratinocytes
3.1.4. Motility of Malignant Cells Was Significantly Impaired by CAP Treatment
3.1.5. Total Protein Expression Modulated by CAP Treatment Reflect Changes in Cell Motility
3.2. Examinations on Tissue Specimen
4. Discussion
5. Conclusions
- Controlled application of CAP may provide a means to kill malignant cells.
- CAP application may be a promising adjuvant treatment option to eliminate minimal residual cancer cells after radical surgery of carcinoma of the head and neck area.
- Investigations towards the underlying mechanism remain to be addressed under consideration of accompanying cell types.
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Declarations
References
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Protein Name | Uniprot ID | HaCaT | HNO97 | Function |
---|---|---|---|---|
Ki67 | P46013 | | →↑ | Proliferation |
NQO1 | P15559 | →↑ | | Nuclear factor erythroid 2-related factor 2 (NRF2) related signalling |
TMX2 | Q9Y320 | ↑ | | NRF2 related signalling |
GSTM3 | P21266 | ↑ | | NRF2 related signalling |
PRDX1,2,4,6 | Q06830 | | | Redox regulation, elimination of peroxides |
SBNO2 | Q9Y2G9 | ↑↑ | ↑ | Transcriptional co- regulation, interleukin signalling |
ILKAP | Q9H0C8 | ↑↑ | | Cell adhesion and growth factor signalling |
AAMP | Q13685 | →↑ | ↓ | Cell migration, angiogenesis |
ROCK2 | O75116 | →↑ | ↓↓ | Regulation of actin cytoskeleton, cell adhesion and motility |
CTTB2 | Q8WZ74 | ↑↑ | →↑ | Cytoskeleton rearrangement, cell migration and motility |
CFA20 | Q9Y6A4 | ↑↑ | | Cell motility |
SRGP2 | O75044 | n.d. | ↑ | Cell migration inhibition, actin dynamics |
ELMO3 | Q96BJ8 | | ↓ | Cell motility |
CASP8/10 | Q14790/Q92851 | | ↑ | Pro-apoptotic caspases |
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Hasse, S.; Seebauer, C.; Wende, K.; Schmidt, A.; Metelmann, H.-R.; von Woedtke, T.; Bekeschus, S. Cold Argon Plasma as Adjuvant Tumour Therapy on Progressive Head and Neck Cancer: A Preclinical Study. Appl. Sci. 2019, 9, 2061. https://doi.org/10.3390/app9102061
Hasse S, Seebauer C, Wende K, Schmidt A, Metelmann H-R, von Woedtke T, Bekeschus S. Cold Argon Plasma as Adjuvant Tumour Therapy on Progressive Head and Neck Cancer: A Preclinical Study. Applied Sciences. 2019; 9(10):2061. https://doi.org/10.3390/app9102061
Chicago/Turabian StyleHasse, Sybille, Christian Seebauer, Kristian Wende, Anke Schmidt, Hans-Robert Metelmann, Thomas von Woedtke, and Sander Bekeschus. 2019. "Cold Argon Plasma as Adjuvant Tumour Therapy on Progressive Head and Neck Cancer: A Preclinical Study" Applied Sciences 9, no. 10: 2061. https://doi.org/10.3390/app9102061