The Protective Effect of Alpha-Mangostin against Cisplatin-Induced Cell Death in LLC-PK1 Cells is Associated to Mitochondrial Function Preservation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture and Cell Viability
2.3. Cell Respirometry
2.4. Total Protein Extract and Western Blot
2.5. Mitochondrial Mass by MitoTracker Green
2.6. Statistical Analysis
3. Results
3.1. αM Prevented CDDP-Induced Cell Death
3.2. αM’s Protection is Associated to the Preservation of Mitochondrial Bioenergetics
3.3. Mitochondrial Respirometry Alterations are Associated to Mitochondrial Mass Decrease Related to Mitochondrial Biogenesis Reduction
3.4. The Induction of Mitophagy is Related to Protection by αM
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Reyes-Fermín, L.M.; Avila-Rojas, S.H.; Aparicio-Trejo, O.E.; Tapia, E.; Rivero, I.; Pedraza-Chaverri, J. The Protective Effect of Alpha-Mangostin against Cisplatin-Induced Cell Death in LLC-PK1 Cells is Associated to Mitochondrial Function Preservation. Antioxidants 2019, 8, 133. https://doi.org/10.3390/antiox8050133
Reyes-Fermín LM, Avila-Rojas SH, Aparicio-Trejo OE, Tapia E, Rivero I, Pedraza-Chaverri J. The Protective Effect of Alpha-Mangostin against Cisplatin-Induced Cell Death in LLC-PK1 Cells is Associated to Mitochondrial Function Preservation. Antioxidants. 2019; 8(5):133. https://doi.org/10.3390/antiox8050133
Chicago/Turabian StyleReyes-Fermín, Laura María, Sabino Hazael Avila-Rojas, Omar Emiliano Aparicio-Trejo, Edilia Tapia, Isabel Rivero, and José Pedraza-Chaverri. 2019. "The Protective Effect of Alpha-Mangostin against Cisplatin-Induced Cell Death in LLC-PK1 Cells is Associated to Mitochondrial Function Preservation" Antioxidants 8, no. 5: 133. https://doi.org/10.3390/antiox8050133