Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Toxoplasma gondii Chimeric Recombinant Proteins and Toxoplasma Lysate Antigen
2.2. Serums Samples of Small Ruminants
2.3. IgG ELISA
2.4. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chimeric Proteins | Amino Acid Residues | Protein Characteristic |
---|---|---|
AMA1N-SAG2-GRA1-ROP1 | 68–287 AMA1 31–170 SAG2 26–190 GRA1 85–396 ROP1 | 899 aa Mw 97.95 kDa |
AMA1C-SAG2-GRA1-ROP1 | 287–569 AMA1 31–170 SAG2 26–190 GRA1 85–396 ROP1 | 961 aa Mw 103.20 kDa |
AMA1-SAG2-GRA1-ROP1 | 68–569 AMA1 31–170 SAG2 26–190 GRA1 85–396 ROP1 | 1182 aa Mw 128.42 kDa |
SAG2-GRA1-ROP1-GRA2 | 31–170 SAG2 26–190 GRA1 185–396 ROP1 51–185 GRA2 | 806 aa Mw 86.32 kDa |
Results of IgG ELISA | Chimeric Recombinant Antigens and TLA | |||||
---|---|---|---|---|---|---|
AMA1N-SAG2-GRA1-ROP1 | AMA1C-SAG2-GRA1-ROP1 | AMA1-SAG2-GRA1-ROP1 | SAG2-GRA1-ROP1-GRA2 | TLA | ||
Sheep Sera | Group IS = 48 A | 1.457 (0.175–2.700) | 1.808 (0.081–2.308) | 1.572 (0.150–2.611) | 1.509 (0.108–2.897) | 1.349 (0.237–2.256) |
Group IIS = 42 A | 0.222 (0.096–0.469) | 0.132 (0.077–0.364) | 0.212 (0.100–0.440) | 0.232 (0.101–0.968) | 0.269 (0.133–0.438) | |
Cutoff | 0.388 | 0.238 | 0.467 | 0.502 | 0.442 | |
Sensitivity | 97.92% | 95.83% | 97.92% | 97.92% | 97.92% | |
Specificity | 97.62% | 95.24% | 100% | 97.62% | 100% | |
AUC (ROC analysis) | 0.9836 | 0.9678 | 0.9836 | 0.9777 | 0.9881 | |
PPV B | 97.92% | 95.83% | 100% | 97.92% | 100% | |
NPV C | 97.62% | 95.24% | 97.67% | 97.62% | 97.67% | |
Goat Sera | Group IG = 45 A | 0.991 (0.184–2.668) | 1.201 (0.304–2.382) | 1.142 (0.343–2.668) | 1.160 (0.179–2.311) | 1.046 (0.427–2.258) |
Group IIG = 41 A | 0.256 (0.127–0.795) | 0.620 (0.304–1.128) | 0.267 (0.127–0.535) | 0.198 (0.109–0.353) | 0.314 (0.196–0.464) | |
Cutoff | 0.546 | 0.336 | 0.534 | 0.533 | 0.489 | |
Sensitivity | 88.89% | 95.56% | 95.56% | 57.78% | 97.78% | |
Specificity | 100% | 97.56% | 100% | 95.12% | 100% | |
AUC (ROC analysis) | 0.9981 | 0.9897 | 0.9900 | 0.8425 | 0.9989 | |
PPV B | 100% | 89.58% | 100% | 92.86% | 100% | |
NPV C | 89.13% | 95.24% | 95.35% | 67.24% | 97.62% | |
All Sera | Sensitivity D | 93.55% | 95.70% | 96.77% | 78.49% | 97.85% |
Specificity E | 98.8% | 97.59% | 100% | 96.39% | 100% | |
PPV B | 98.86% | 97.80% | 100% | 96.05% | 100% | |
NPV C | 93.18% | 95.29% | 97.65% | 80% | 97.65% |
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Holec-Gąsior, L.; Ferra, B.; Grąźlewska, W. Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants. Animals 2019, 9, 1146. https://doi.org/10.3390/ani9121146
Holec-Gąsior L, Ferra B, Grąźlewska W. Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants. Animals. 2019; 9(12):1146. https://doi.org/10.3390/ani9121146
Chicago/Turabian StyleHolec-Gąsior, Lucyna, Bartłomiej Ferra, and Weronika Grąźlewska. 2019. "Toxoplasma gondii Tetravalent Chimeric Proteins as Novel Antigens for Detection of Specific Immunoglobulin G in Sera of Small Ruminants" Animals 9, no. 12: 1146. https://doi.org/10.3390/ani9121146