Rumen Microbiota Transplantation Alleviates Gossypol Diet-Induced Reproductive, Liver, and Intestinal Damage in Male Mice
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Feeding of Mice and FMT
2.2. Clinical Information
2.3. Sample Collection
2.4. Analysis of Liver Injury
2.5. Reproductive Injury Analysis
2.6. Intestinal Tissue Analysis
2.7. Data Analysis
3. Results
3.1. Effects of Rumen Microbiota Transplantation on Body Weight and Food Intake of Mice
3.2. Effects of Rumen Microbiota Transplantation on the Organ Coefficients of Mice
3.3. Effects of Rumen Microbiota Transplantation on Liver Function in Mice
3.4. Effect of Rumen Microbiota Transplantation on the Reproductive Function of Mice
3.5. Effect of Rumen Microbiota Transplantation on the Small Intestine of Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Treatment | Description |
---|---|---|
CK | Fed a gossypol-free diet; oral administration of CMC-Na solution | Blank control group |
FG | Fed a gossypol diet (CM diet, FG = 100.06 ± 0.42 mg/kg); oral administration of CMC-Na solution | Negative control group |
FMT | Fed a gossypol diet (FG = 100.06 ± 0.42 mg/kg); oral administration of FMT oral solution | Rumen microbiota transplant group |
Treatments | n | Injury Level | Score | Ridit-Value | ||||
---|---|---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | 4 | ||||
CK | 10 | 8 | 1 | 1 | 0 | 0 | 3 | 7.50 c |
FG | 10 | 0 | 0 | 3 | 5 | 2 | 29 | 24.20 a |
FMT | 10 | 2 | 3 | 4 | 1 | 0 | 14 | 14.80 b |
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Zhang, C.; Lu, W.; Liu, H.; Shen, L.; Zhu, M.; Zhou, T.; Zhang, L.; Xiao, D.; Chen, L. Rumen Microbiota Transplantation Alleviates Gossypol Diet-Induced Reproductive, Liver, and Intestinal Damage in Male Mice. Animals 2024, 14, 2206. https://doi.org/10.3390/ani14152206
Zhang C, Lu W, Liu H, Shen L, Zhu M, Zhou T, Zhang L, Xiao D, Chen L. Rumen Microbiota Transplantation Alleviates Gossypol Diet-Induced Reproductive, Liver, and Intestinal Damage in Male Mice. Animals. 2024; 14(15):2206. https://doi.org/10.3390/ani14152206
Chicago/Turabian StyleZhang, Chen, Wenguang Lu, Huiru Liu, Lingwei Shen, Mengfan Zhu, Tangtang Zhou, Ling Zhang, Dingfu Xiao, and Lijuan Chen. 2024. "Rumen Microbiota Transplantation Alleviates Gossypol Diet-Induced Reproductive, Liver, and Intestinal Damage in Male Mice" Animals 14, no. 15: 2206. https://doi.org/10.3390/ani14152206