Porcine Germ Cells Phenotype during Embryonic and Adult Development
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Sexing of Collected Embryos
2.3. Histological Analysis
2.4. Immunofluorescence Analysis
2.5. qRT-PCR
2.6. Statistical Analysis
3. Results
3.1. Morphological and Immunofluorescence Analysis
3.1.1. Male Gonads
3.1.2. Female Gonads
3.2. Analysis of the Gene Expression in Porcine Germ Cells
4. Discussion
4.1. Histological and Immunophenotype Analysis of the Porcine Germ Cells
4.2. Epigenetic Markers in Porcine Germ Cells
4.3. Analysis of the Gene Expression in Porcine Germ Cells
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sequence Name | Sequence | Size (bp) | Gene Bank |
---|---|---|---|
OCT4 (POU5F1) | GGGTTCTCTTTGGGAAGGTGT CTCCAGGTTGCCTCTCACTC | 224 | NM_001113060.1 |
SOX2 | CTCAGTGGTCAAGTCCGAGG AGAGAGAGGCAGTGTACCGT | 223 | NM_001123197.1 |
NANOG | TCCTTCCTCCATGGATCTGCT GGGTCTGCGAGAACACAGTT | 155 | NM_001129971.1 |
SOX17 | TCCACTCTGCTAGTGCCTCT CTGGGGATGCCCTAATGTTCA | 193 | XM_001928376.7 |
TFAP2C | GAAACCCTGGACTGGACGAG GTAGCACCACTTGCAGAGGA | 121 | NM_001123201 |
PRDM14 | AGTGGATGCTTCTCTGCTACC TGCCTTTCTCTCTTGGTTCA | 112 | [26] |
BLIMP1 (PRDM1) | CAGTGCCGTGAAGTTTCC AAGGATGCCTCTGCCTGAAC | 186 | [26] |
VASA (DDX4) | CCTGCCCAGGAATGCCATCA ACTGGCCAACTTGGAGAATGGT | 180 | [26] |
STELLA (Dapp3) | CCCGCCTTTCAATCTGTCTCC TCGCCGAACCGTGTATCGAA | 219 | [26] |
DAZL | GGTCGCTTTGCTTATCCGC TGCAGCAGACATTACTGCGA | 158 | [26] |
STRA8 | TGGAGAAGGGAGCAACCCCA ACCTGCCACTTTGAGGCTGT | 189 | [26] |
β-ACTIN | GAAGATCAAGATCATCGCGCCT GTGGAATGCAACTAACAGTCCG | 117 | XM_003124280.4 |
GAPDH | GTCGGTTGTGGATCTGACCT ACCAGGAAATGAGCTTGACGA | 221 | NM_001206359.1 |
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Jorge, A.S.; Recchia, K.; Glória, M.H.; de Souza, A.F.; Pessôa, L.V.d.F.; Fantinato Neto, P.; Martins, D.d.S.; de Andrade, A.F.C.; Martins, S.M.M.K.; Bressan, F.F.; et al. Porcine Germ Cells Phenotype during Embryonic and Adult Development. Animals 2023, 13, 2520. https://doi.org/10.3390/ani13152520
Jorge AS, Recchia K, Glória MH, de Souza AF, Pessôa LVdF, Fantinato Neto P, Martins DdS, de Andrade AFC, Martins SMMK, Bressan FF, et al. Porcine Germ Cells Phenotype during Embryonic and Adult Development. Animals. 2023; 13(15):2520. https://doi.org/10.3390/ani13152520
Chicago/Turabian StyleJorge, Amanda Soares, Kaiana Recchia, Mayra Hirakawa Glória, Aline Fernanda de Souza, Laís Vicari de Figueirêdo Pessôa, Paulo Fantinato Neto, Daniele dos Santos Martins, André Furugen Cesar de Andrade, Simone Maria Massami Kitamura Martins, Fabiana Fernandes Bressan, and et al. 2023. "Porcine Germ Cells Phenotype during Embryonic and Adult Development" Animals 13, no. 15: 2520. https://doi.org/10.3390/ani13152520