Direct Inhibition of T-Cell Responses by the Cryptococcus Capsular Polysaccharide Glucuronoxylomannan
Figure 7
GXM from Four Cryptococcal Serotypes Inhibits Proliferation of Human PBMCs
PBMCs were activated with tetanus toxoid (TT) in the presence or absence of GXM (100, 300, or 1,000 μg/ml) isolated from serotype A strain Cn6, serotype A strain H99, serotype B strain R265, serotype C strain Cn18, or serotype D strain B3501. Proliferation was measured by [3H]thymidine incorporation 6 d later. Data are expressed as the percent proliferation compared with TT alone (−) (set at 100%) and are expressed as the mean ± SEM of four donors (or two donors for strain Cn6 GXM) assayed in triplicate. The mean CPM for TT in the absence of GXM was 63,550. *p ≤ 0.005; **p < 0.0001.
doi: https://doi.org/10.1371/journal.ppat.0020120.g007