Granzyme A Is Required for Regulatory T-Cell Mediated Prevention of Gastrointestinal Graft-versus-Host Disease
Fig 3
GzmA-/- Tregs are highly suppressive in vitro.
Regulatory T cells isolated from WT (A) or gzmA-/- (B) mice were co-cultured with CD4+CD25- T responder cells and activated with αCD3/αCD28. The proliferation of the responder cells was measured by the dilution of the proliferation dye CFSE. (A) and (B) show the proliferative response with or without (grey) regulatory T cells in a ratio 1:1. Percentage of suppression is shown for WT and gzmA-/- Tregs in different effector T cell to regulatory T cell ratios (C).
doi: https://doi.org/10.1371/journal.pone.0124927.g003