Identification of the Porcine G Protein-Coupled Receptor 41 and 43 Genes and Their Expression Pattern in Different Tissues and Development Stages
Figure 1
Expression of GPR41 and GPR43 mRNA in different porcine tissues at 160 day-old.
The reverse transcription in the absence of reverse transcriptase (RTse –) was used to control for amplification from incompletely digested genome DNA. The GAPDH mRNA served as a loading control. The sizes of GPR41, GPR43 and GAPDH PCR products were 398 bp, 249 bp and 220 bp, respectively. The bands of the marker were 50 bp, 100 bp, 150 bp, 200 bp, 300 bp, 400 bp, and 500 bp from bottom to above. M: marker; L: liver; S: spleen; I: ileum; C: colon; H: heart; K: kidney; AT: adipose tissue; SM: skeletal muscle.
doi: https://doi.org/10.1371/journal.pone.0097342.g001