Phosphodiesterase 3/4 Inhibitor Zardaverine Exhibits Potent and Selective Antitumor Activity against Hepatocellular Carcinoma Both In Vitro and In Vivo Independently of Phosphodiesterase Inhibition
Figure 5
The selective antitumor activity of zardaverine is closely related to the regulation of cell cycle-associated proteins.
Bel-7402 and SMMC-7721 cells (A), SNU-739 and HCT 116 cells (B) were incubated with zardaverine for 24 h. Then whole-cell lysates were prepared and analyzed by Western blotting. C, Cells were seeded in 6-well plates overnight. Whole-cell lysates were prepared, then total Rb levels were determined by Western blotting. IC50 values of zardaverine for these cells listed here are from Table 1.
doi: https://doi.org/10.1371/journal.pone.0090627.g005