Lipopolysaccharides Impair Insulin Gene Expression in Isolated Islets of Langerhans via Toll-Like Receptor-4 and NF-κB Signalling
Figure 2
Exposure to LPS decreases insulin, PDX-1 and MafA gene expression in isolated islets via TLR4.
(A) Insulin pre-mRNA expression (B) PDX-1 mRNA expression and (C) MafA mRNA expression in islets isolated from WT and TLR4-deficient mice exposed for 24 h to 2.8 (2.8 G) and 16.7 mM (16.7 G) glucose in the presence or absence of 0.5 mM palmitate (PA), 0.5 ng/mL IL-1β or 5 µg/mL LPS. mRNA levels were measured by RT-PCR and normalized to β-actin mRNA levels. Data are mean ± S.E.M. of 6 independent experiments; *p<0.05.
doi: https://doi.org/10.1371/journal.pone.0036200.g002