ZIP8 Zinc Transporter: Indispensable Role for Both Multiple-Organ Organogenesis and Hematopoiesis In Utero
Figure 3
Western immunoblot analysis of ZIP8 protein (50.082 kDa) in the same tissues described in Fig. 2
. (A) Whole embryo/fetus and yolk sac; (B) Four tissues at PND1. Antibody to β-actin protein (ACTB; 41.7 kDa) was used as the lane-loading control. To maximize contrast, exposure times ranged from 1 min to 6 h. (C) Densitometric semi-quantification of Western immunoblots: whole embryo/fetus, yolk sac and four tissues at PND1. The Slc39a8(+/+) wild-type ZIP8 protein/ACTB protein ratio (left-most in each panel) and protein expression levels were expressed as linear fold-changes–relative to the normalized wild-type control.
doi: https://doi.org/10.1371/journal.pone.0036055.g003