A Filamentous Hemagglutinin-Like Protein of Xanthomonas axonopodis pv. citri, the Phytopathogen Responsible for Citrus Canker, Is Involved in Bacterial Virulence
Figure 3
Adhesion of X. axonopodis pv. citri wild type and ΔXacFhaB and ΔXacFhaC strains.
(A) Bacterial adhesion on plastic (PVC) surface of XacWT, ΔXacFhaB and ΔXacFhaC mutant strains grown in SB or XVM2 medium. In the left, a representative image of CV stain is shown. In the right, bars are the mean of CV stain measured spectrophotometrically (Abs. 570 nm) and error bars are standard deviations. The means were calculated from 20 wells (plastic) for each sample, the results are representative of three independent experiments. (B) Bacterial adhesion on abaxial leaves surfaces. In the left, a representative image of CV stain on a leaf is shown while in the right bars represent the mean of CV from 20 stained leaf spots measured as in (A) and error bars are standard deviations. (C) Adhesion of ΔXacFhaB to the leaf surface trough the co-incubation of this mutant strain with XacWT XVM2 supernatant (C1) or with XacWT cells grown in XVM2 in a relation 1∶1 (C2) without or with proteinase K (PK). Bars represent the mean of CV from 25 stained leaf spots measured as in (A) and error bars are standard deviations.
doi: https://doi.org/10.1371/journal.pone.0004358.g003