Coordination of Flower Maturation by a Regulatory Circuit of Three MicroRNAs
Figure 4
Interaction of miR159 and miR319 targets.
(A) Comparison of 45-day old plants. (B) Close-up of flowers in single and double Pro35S:MIM159 and Pro35S:MIM319 expressing plants. (C) Altered floral development as consequence of reduced miR159 activity and/or miR319 overexpression. (D) ARF6/8 expression levels in single and double Pro35S:MIM159 and Pro35S:MIM319 plants as monitored by real-time RT-PCR. Error bars indicate range of two biological and two technical replicates. Measurements in mutants were normalized to values from wild-type inflorescences (dashed lines). (E) Interaction of miR159 target MYB33 and miR319 target TCP4 in yeast-two-hybrid system. (F) Interaction of MYB33 and TCP4 assayed by firefly luciferase complementation in N. benthamiana. Luciferase activity is shown in false color, with highest levels red and lowest levels blue. Scale bars indicate 1 mm (B,C).
doi: https://doi.org/10.1371/journal.pgen.1003374.g004