Abstract

The fluorescence decay of high-affinity nonratiometric ${\mathrm{Ca}}^{2+}$ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free ${\mathrm{Ca}}^{2+}$ concentration and dissociation constants of nonratiometric calcium indicators.

© 2009 Optical Society of America

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