Molecular characterization of Cryptosporidium parvum detected in Japanese black and Holstein calves in Iwate Prefecture and Tanegashima Island, Kagoshima Prefecture, Japan

Junya AITA; Madoka ICHIKAWA-SEKI; Aiko KINAMI; Seiko YAITA; Yoshihiro KUMAGAI; Yoshifumi NISHIKAWA; Tadashi ITAGAKI

doi:10.1292/jvms.15-0082

Parasitology

Molecular characterization of Cryptosporidium parvum detected in Japanese black and Holstein calves in Iwate Prefecture and Tanegashima Island, Kagoshima Prefecture, Japan

Junya AITA, Madoka ICHIKAWA-SEKI, Aiko KINAMI, Seiko YAITA, Yoshihiro KUMAGAI, Yoshifumi NISHIKAWA, Tadashi ITAGAKI

Author information

Junya AITA
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
Madoka ICHIKAWA-SEKI
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
Aiko KINAMI
Apartment House Iris B101, 142–12 Shinogikurohata, Takizawa, Iwate 020–0735, Japan
Seiko YAITA
Kumage Agricultural Mutual Aid Association, Noma-6410–8 Nakatane-cho, Kumage, Kagoshima 891–3604, Japan
Yoshihiro KUMAGAI
Iwate Prefecture Southern Regional Livestock Hygiene Service Center, 41–1 Higashidate, Sakurakawa, Mizusawa-ku, Oushu, Iwate 020–0003, Japan
Yoshifumi NISHIKAWA
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi-2–13 Inada-cho, Obihiro, Hokkaido 080–8555, Japan
Tadashi ITAGAKI
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan

Keywords: calf, Cryptosporidium parvum, GP60, Japan, subtyping

JOURNAL FREE ACCESS

2015 Volume 77 Issue 8 Pages 997-999

DOI https://doi.org/10.1292/jvms.15-0082

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Abstract

Cryptosporidium oocysts were found in 43 out of 77 calves from two farms in Iwate Prefecture and nine farms on Tanegashima Island, Kagoshima Prefecture, Japan. The DNA fragments of 18S ribosomal RNA (18S rRNA) gene were amplified by a nested PCR from 43 oocyst-positive as well as one oocyst-negative samples. All of them were precisely identified as C. parvum by analyzing the nucleotide sequences of the 18S rRNA gene. C. parvum oocyst-positive calves ranged in age from 6 to 13 days old and significantly have watery diarrhea (P<0.05). Sequences of the gene encoding the 60-kDa glycoprotein (GP60) in 43 Cryptosporidium oocyst-positive samples were identical to that of the zoonotic IIaA15G2R1 subtype. We therefore suggest that calves could be potential sources of C. parvum infections in humans.

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