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Prenatal exposure to the organophosphate insecticide chlorpyrifos enhances brain oxidative stress and prostaglandin E2 synthesis in a mouse model of idiopathic autism

Journal of Neuroinflammation volume 13, Article number: 149 (2016) Cite this article

Abstract

Background

Autism spectrum disorders (ASD) are emerging as polygenic and multifactorial disorders in which complex interactions between defective genes and early exposure to environmental stressors impact on the correct neurodevelopment and brain processes. Organophosphate insecticides, among which chlorpyrifos (CPF), are widely diffused environmental toxicants associated with neurobehavioral deficits and increased risk of ASD occurrence in children. Oxidative stress and dysregulated immune responses are implicated in both organophosphate neurodevelopmental effects and ASD etiopathogenesis. BTBR T+tf/J mice, a well-studied model of idiopathic autism, show several behavioral and immunological alterations found in ASD children, and we recently showed that CPF gestational exposure strengthened some of these autistic-like traits. In the present study, we aimed at investigating whether the behavioral effects of gestational CPF administration are associated with brain increased oxidative stress and altered lipid mediator profile.

Methods

Brain levels of F2-isoprostanes (15-F2t-IsoP), as index of in vivo oxidative stress, and prostaglandin E2 (PGE2), a major arachidonic acid metabolite released by immune cells and by specific glutamatergic neuron populations mainly in cortex and hippocampus, were assessed by specific enzyme-immuno assays in brain homogenates from BTBR T+tf/J and C57Bl6/J mice, exposed during gestation to either vehicle or CPF. Measures were performed in mice of both sexes, at different postnatal stages (PNDs 1, 21, and 70).

Results

At birth, BTBR T+tf/J mice exhibited higher baseline 15-F2t-IsoP levels as compared to C57Bl6/J mice, suggestive of greater oxidative stress processes. Gestational treatment with CPF-enhanced 15-F2t-IsoP and PGE2 levels in strain- and age-dependent manner, with 15-F2t-IsoP increased in BTBR T+tf/J mice at PNDs 1 and 21, and PGE2 elevated in BTBR T+tf/J mice at PNDs 21 and 70. At PND 21, CPF effects were sex-dependent being the increase of the two metabolites mainly associated with male mice. CPF treatment also induced a reduction of somatic growth, which reached statistical significance at PND 21.

Conclusions

These findings indicate that the autistic-like BTBR T+tf/J strain is highly vulnerable to environmental stressors during gestational period. The results further support the hypothesis that oxidative stress might be the link between environmental neurotoxicants such as CPF and ASD. The increased levels of oxidative stress during early postnatal life could result in delayed and long-lasting alterations in specific pathways relevant to ASD, of which PGE2 signaling represents an important one.

Background

The etiological bases of the majority of human neurodevelopmental disorders, including autism spectrum disorders (ASD), are still unknown though a great body of data supports their polygenic and multifactorial etiology [1]. Substantial evidence indicates that ASD may derive from the complex interaction between many defective genes and early exposure to different environmental stressors, which can alter the typical neurodevelopment with consequent disruption of some behaviors [2].

Dysregulated immune responses and lipid metabolism impairment, associated with enhanced oxidative stress, are thought to contribute to etiopathogenesis of ASD and other neurodevelopmental disorders [3–5]. In particular, some data suggest that alterations in lipid metabolism, due to multiple causes, play an important role in autism pathogenesis [6, 7]. Oxidative stress, a condition defined as an alteration in the balance between pro-oxidant and anti-oxidant molecules and associated with long-term damage [8], is one of the plausible mechanisms that link genes and environment. Several data point to a key role of oxidative stress in human toxicity of industrial chemicals, such as heavy metals and pesticides [9–11]; in particular, induction of oxidative stress, besides acetylcholinesterase (AChE) inhibition, is among the main mechanisms mediating both acute and chronic organophosphate (OP) pesticide exposure [9, 10, 12]. The human brain is composed predominantly by lipids; polyunsaturated fatty acids (PUFAs) such as arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid are major constituents of the cell membranes. PUFAs can be metabolized through different enzymatic pathways to generate lipid signaling messengers [13]. Among them, prostaglandin E2 (PGE2), an arachidonic acid metabolite of the cyclooxygenase (COX) pathway, is an important lipid signaling messenger affecting many immune and brain functions [14].

PUFAs are exquisitely sensitive to oxidative stress, and broad range metabolites can be generated non-enzymatically, by direct free radical attack of membrane esterified PUFAs [15]. Among these lipid peroxidation products, F2-isoprostanes, and in particular 15-F2t-Isoprostane (15-F2t-IsoP, also known as 8-isoprostane-F2α), are considered reliable index of in vivo oxidative stress. Of note, increased levels of 15-F2t-IsoP have been detected in both red blood cells and urine samples of children with autism compared to age-matched controls [16]; later, El-Ansary and co-workers found elevated plasma F2-isoprostanes in Saudi autistic children, together with alterations in lipid mediators such as PGE2 and leukotrienes [17].

Black and tan brachyury T+tf/J (BTBR) is an inbred mouse strain that displays several behavioral traits relevant to autism, such as impairments in social and communication domains with lacks of sociability in social approach tasks, reduction in the emission of ultrasonic vocalizations in various social settings, and high levels of repetitive behaviors [18]. Although well-studied, the inherited genetic changes that lead to autistic-like behaviors in these mice are scarcely known and still under active investigation [19–21]. BTBR mice show several of the immunological alterations found in children with ASD, such as an increased rate of immunological activation, with high levels of pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α and increased number of microglia at the adult stage [22, 23].

The link between immune system dysregulation and behavioral deficits in ADS is not yet well understood, and the BTBR mouse provides a well-established model to further investigate this issue. Immune disturbances found in this mouse strain might result in higher vulnerability to oxidative stress promoted by toxicants, with possible more severe repercussions on neural development, a hypothesis that has also called in to explain the environmental contribution to autism risk in children [24].

To test this hypothesis, in the present study, we administered the OP pesticide chlorpyrifos (CPF) at the sub-toxic dose of 6 mg/kg/body weight or vehicle to pregnant mice of either C57 BL6/J (C57) or BTBR inbred strains, by oral gavage, from gestational day (GD) 14 to 17, replicating as for dose and administration schedule our previous studies performed in CD-1 outbred mice [25, 26]. Among OP pesticides, CPF has recently been listed as one of the environmental chemicals possibly responsible for increased ASD risk in children [27]. Specifically, epidemiological studies indicate that environmentally relevant exposure in utero to CPF may affect children’s neuropsychological maturation, in terms of impaired reflex functioning in newborns [28, 29] and decreased mental and psychomotor performances and attention problems in infants, with higher risk to develop pervasive developmental disorders [30]. In agreement with human data, an increasing body of rodent data indicates that, at sub-toxic doses, developmental exposure to CPF affects neurobehavioral maturation, targeting neural and neuroendocrine systems involved in ASD etiology [31–35]. In the present study, C57 mice were used as control strain since they present strong similarity with BTBR mice as for gene background, but lack the autism-like phenotype [19]. 15-F2t-IsoP and PGE2 were measured in the whole brain at postnatal day (PND) 1 and 70 in the offspring of both sexes in the two strains. The same parameters were measured at weaning (PND 21) in the BTBR strain. BTBR mice showed higher baseline levels of 15-F2t-IsoP at birth as compared to C57 mice, suggestive of enhanced oxidative stress processes. In line with our hypothesis, gestational CPF treatment selectively enhanced oxidative stress in BTBR mice at birth (PND 1) and weaning (PND 21), whereas PGE2 levels were particularly elevated at adulthood (PND 70).

Methods

Animals

Male and female mice of the BTBR and C57 strain purchased from the Jackson Laboratory (Bar Harbour, ME, USA) were housed upon arrival in breeding cages (polycarbonate cages 33x13x14 cm) under standard animal housing conditions (temperature 20 ± 2 °C; humidity 60–70 %) with food (enriched standard diet for mice from Altromin, Spezialfutter GmbH & Co. Germany) and water ad libitum, under a 12:12 reverse light cycle (lights on from 8:00 p.m. till 8:00 a.m.). Females were inspected daily for the presence of the vaginal plug (GD 0). On GD 14, pregnant females were randomly assigned to one of the two prenatal treatments (vehicle, CPF). CPF (Chem. Service, West Chester, PA) was dissolved in peanut oil as vehicle to provide rapid and complete absorption. CPF (in a volume of 0.1 ml/10 g at a dose of 6 mg/kg/bw) or its vehicle was administered to pregnant females from GD 14 to 17 by intraoral gavage. The dose of CPF was originally selected on the basis of previous multidose studies performed in our laboratory in CD1 mice [26, 36, 37] as the more effective one in producing behavioral changes in the absence of overt toxic symptoms in dams or major effects on pregnancy length, number of pups at delivery, sex ratio, pups’ weight at delivery and growing rate. This same dose was able to induce significant changes in early motor development and amplification of the autism-like behavioral traits in adult males in BTBR strain (see below). A total of 31 litters (16 vehicle-treated and 15 CPF-treated) were used. Females’ body weight was monitored daily during pregnancy. Proportion of term pregnancies, gestation length, litter size, sex ratio, and neonatal mortality were also measured to exclude potential effects of the treatment on reproductive performances. On the day of birth, which is defined as PND 0, the sex of the pups was assessed by evaluation of anogenital distance. One pup of each sex was randomly selected from each litter and sacrificed at either PND 1, PND 21 (BTBR only), or PND 70 for the assessment of 15-F2t-IsoP and PGE2 in the brain. The siblings of the BTBR mice used in the present study formed the subjects of a different study aimed at describing early and delayed effects of CPF on the behavioral phenotype of BTBR mice [25]. At weaning (PND 21), male and female offspring were separated and maintained in same-sex pairs till the adulthood. Body weight of each individual pup was recorded on PNDs 1, 4, 6, 8, and 12, at weaning (PND 21) and at adulthood (PND 70). In addition, nine C57 mice and ten BTBR mice born from mothers not receiving any treatment were used to measure the baseline levels of both 15-F2t-IsoP and PGE2 in the two strains on the day of birth.

15-F2t-IsoP and PGE2 measurement

The levels of 15-F2t-IsoP were assessed as previously described [38]. Briefly, brains were weighed and homogenized in 50-mM Tris buffer, pH 7.5 (1 mg/0.1 ml), containing the anti-oxidant BHT (10 μM) and the COX inhibitor indomethacin (1 μM) to block ex vivo arachidonic acid auto-oxidation and PGs formation. Homogenates were vigorously vortexed and incubated for 5 min on ice before centrifuging at 14,000 rpm for 45 min at 4 °C. Supernatants were collected and stored at −80 °C until assayed. 15-F2t-IsoP was measured by a specific competitive enzyme immunoassay (Cayman Chemical Company, Ann Arbor, MI), according to the manufacturer’s instructions. Ellman’s reagent was used as a substrate and absorbance measured at 405 nm using a microplate reader (GDV DV990BV6). For quantification, a standard curve was built with eight serial dilutions ranging from 500 to 0.8 pg/ml and analysis performed using four-parameter logistic fitting. Detection limit was 2 pg/ml; anti-15-F2t-IsoP antibody cross-reactivity with other iso-prostaglandins was less than 0.15 %. PGE2 levels were measured by a competitive high sensitivity enzyme immunoassay (sensitivity 8.25 pg/ml; Assay Design, Inc., Ann Arbor, MI). A buffered solution of p-nitrophenyl phosphate was used as substrate and absorbance measured at 405 nm with correction at 570 nm, using a microplate reader (GDV DV990BV6). For quantification, a standard curve was built with eight serial dilution ranging from 1000 to 7.8 pg/ml and analysis performed using four-parameter logistic fitting. Anti-PGE2 antibody cross-reactivity with other prostaglandins was less than 1.5 %. Serial dilutions of samples were then tested, in duplicate, for 15-F2t-IsoP and PGE2 content.

Statistical analysis

Data are expressed as means ± SEM of n independent experiments (run in duplicate). Due to the large difference in baseline levels between birth and adulthood, each age point was analyzed separately. Statistical significance was evaluated applying factorial ANOVA with strain (2 levels), treatment (2 levels), and sex (2 levels) as fixed grouping factors. Multiple comparisons were performed by the Tukey HSD test. Correlation coefficients (r s) were calculated by Spearman’s rank correlation. p < 0.05 was accepted as statistical significance. Data were analyzed using the Stata Tm 10 statistical package (Stata Corporation, College Station, TX, USA).

Results

Brain levels of 15-F2t-IsoP and PGE2 in BTBR and C57BL6/J newborn mice

Firstly, we compared brain levels of both 15-F2t-IsoP and PGE2 at PND 1 in BTBR and C57 mice of both sexes born to untreated mothers (Fig. 1). BTBR mice showed higher 15-F2t-IsoP levels than C57 mice [main effect of strain: F(1,14) = 20.607, p = 0.0005], suggesting that, in line with the observations reported in ASD children, BTBR mice are characterized by higher levels of oxidative stress. PGE2 levels were not significantly different between the two strains of mice, but they were higher in female offspring of both strains [main effect of sex: F(1,14) = 6.029, p = 0.027].

Fig. 1
figure 1

Brain levels of 15-F2t-IsoP and PGE2 in untreated male and female C57BL6/J and BTBR mice at PND 1. Levels of 15-F2t-IsoP or PGE2 in brain homogenates are given as pg/mg of wet tissue and are expressed as mean ± SEM (n = 4 males and 5 = females for both BTBR and C57BL6/J)

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Effects of CPF prenatal exposure in BTBR and C57BL6/J mice

In agreement with our previous studies, the selected dose for CPF treatment (6 mg/kg/bw) did not affect reproductive parameters such as gestation length, weight gain of the pregnant females, and weight and viability of the pups at birth, in both strains [25].

The effect of gestational CPF administration on brain levels of 15-F2t-IsoP and PGE2 is shown in Fig. 2. As for 15-F2t-IsoP (Fig. 2a), at PND 1, ANOVA yielded a significant effect of strain [F(1,31) = 17.519, p = 0.0002] with BTBR mice showing higher levels of 15-F2t-Isop than C57 mice and a significant two-way interaction between strain and treatment [F(1,31) = 10.323, p = 0.0031]. BTBR mice prenatally exposed to CPF exhibited increased levels of 15-F2t-IsoP as compared to vehicle-exposed BTBR mice (p < 0.05 after post hoc comparisons), while CPF treatment tended to decrease 15-F2t-IsoP brain levels in C57 strain (this difference just missed statistical significance). Brain levels of PGE2 were significantly higher in C57 than in BTBR mice (Fig. 2b) [F(1,31) = 36.75, p < 0.0001] and were not influenced by CPF treatment in either strain. In BTBR pups born to vehicle-treated mothers, the levels of both 15-F2t-IsoP and PGE2 were decreased as compared to the offspring of untreated mothers (see Fig. 1), an effect that could be related to reported higher susceptibility to handling stress of BTBR than C57 mice [39]; the effect was, however, transient, and no differences were appreciated between offspring of naive and vehicle-treated mothers at later time points (not shown).

Fig. 2
figure 2

Effects of CPF prenatal exposure on 15-F2t-IsoP and PGE2 brain levels in C57BL6/J and BTBR mice at PNDs 1, 21, and 70. Brain levels of 15-F2t-IsoP (a) and PGE2 (b) were measured in brain homogenates of C57BL6/J (PND 1 and 70) and BTBR mice (PND 1, 21, and 70) prenatally exposed to CPF or vehicle from GD 14 to 17. Data are given as pg/mg of wet tissues and are expressed as mean ± SEM; *p < 0.05 significant difference between vehicle and CPF treatment (n = 10–12 for BTBR and n = 6 for C57BL6/J).

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At PND 70, we found a significant main effect of the strain [F(1,26) = 9.947, p = 0.004] as BTBR mice had higher levels of 15-F2t-IsoP than C57 mice, regardless the treatment received during gestation (Fig. 2a, right panel). PGE2 levels (Fig. 2b) were still higher in C57 than in BTBR strain [main effect of strain F(1,24) = 13.18, p = 0.0013], but ANOVA yielded a significant interaction between strain and treatment [F(1,24) = 6.329, p = 0.019], as CPF induced a significant increase of PGE2 in the BTBR strain (p < 0.05 after post hoc comparisons) and a decrease, which did not reach significance, in the C57 strain.

At PND1 and PND 70, there were no sex differences for either 15-F2t-IsoP or PGE2 levels in both CPF- and vehicle-exposed (BTBR or C57 groups, not shown).

Effects of CPF prenatal exposure in BTBR mice at PND 21

On the basis of above findings showing different profiles of effects at the neonatal stage and adulthood in BTBR mice only, we sought to further investigate the effect of gestational CPF treatment in this strain at PND 21. This age corresponds in laboratory mice to the adolescent phase, namely to a critical transition point in which the second wave of neuronal reorganization occurs, through mechanisms of pruning to eliminate the synapses in excess [40, 41]. We found that at this age, CPF treatment significantly increased the levels of both 15-F2t-IsoP and PGE2 (Fig. 2). For both 15-F2t-IsoP and PGE2, ANOVA yielded a significant main effect of treatment at this age [15-F2t-IsoP: F(1,10) = 11.468, p = 0.0069; PGE2: F(1,10) = 12.309, p = 0.0056]. Furthermore, in the case of 15-F2t-IsoP (Fig. 3a), a significant interaction between treatment and sex [F(1,10) = 7.025, p < 0.024] evidenced that the effect of CPF was limited to the male sex (p < 0.05 after post hoc comparisons). Since a similar trend was observed also for PGE2, we decided to perform post hoc comparisons on the interaction between sex and treatment [F(1,9) = 2.486, p = 0.14], as the Tukey HSD test can be used also in the absence of significant ANOVA results [42]. We found that the effects of CPF were limited to the male sex also in the case of PGE2 (p < 0.05 after comparison between vehicle and CPF within the male group).

Fig. 3
figure 3

Effects of CPF prenatal exposure in male and female BTBR mice at PND 21. Brain levels of 15-F2t-IsoP (a) and PGE2 (b) were measured in brain homogenates of male and female BTBR mice prenatally exposed to CPF or vehicle. Data are given as pg/mg of wet tissues and are expressed as mean ± SEM; #p < 0.05 vs vehicle-exposed mice (n = 6–8 per group)

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Effects of CPF prenatal exposure on somatic growth in BTBR and C57BL6/J mice

In a previous study, we monitored the effect of CPF prenatal exposure on somatic growth of BTBR mice between PND 4 and 12, showing a negative trend and significant effect at PND 12 [25]. As shown in Table 1, body and brain weight of both C57 and BTBR mice prenatally exposed to CPF was not significantly different from that of vehicle-exposed mice at PND 1 and PND 70. At PND 21, body weight of BTBR mice prenatally exposed to CPF was significantly lower than that of vehicle-exposed mice at this age [F(1,12) = 10.99, p = 0.0062], while no significant effects were observed on brain weight (Fig. 3b, right). When considering all three ages, 15-F2t-IsoP and PGE2 brain levels and brain weights in BTBR mice were positively correlated in both vehicle (r s 0.57, p = 0.0011 and r s 0.60, p = 0.0003, respectively)- and CPF-exposed mice (r s 0.76, p = 0.0000; r s 0.81, p = 0.0001, respectively). Similarly, in C57 mice, 15-F2t-IsoP and PGE2 brain levels and brain weights were positively correlated in both vehicle (r s 0.67, p = 0.017 and r s 0.68, p = 0.0153, respectively)- and CPF-exposed mice (r s 0.84, p = 0.003; r s 0.7, p = 0.007, respectively).

Table 1 Somatic and brain growth in C57BL6/J and BTBR mice prenatally exposed to CPF
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Somatic and brain growth were similar in both sexes (not shown).

Discussion

Raising evidence points to oxidative stress as a major pathogenic mechanism shared by etiological determinants of neurodevelopment disorders such as genetic and environmental factors. Several data confirm that induction of oxidative stress is a major contributor of the overall toxicity of the OP pesticides [43]. In particular, acute exposure of adult rodents to high doses of CPF has been reported to increase lipid peroxidation and to decrease the levels of key enzymes implicated in oxidative defenses [44–47]. However, recent epidemiological evidence raised concern on the impact that the prolonged exposure to apparently non-toxic doses of OP in critical phases of brain maturation might have on child’s neurodevelopment. Experimental models mimicking the human exposure scenarios to this widely diffused class of pesticides may be pivotal to elucidate the mechanisms implicated in OP developmental neurotoxicity and identify novel biomarkers for risk assessment.

To the best of our knowledge, this is the first study analyzing the effect of gestational exposure to a sub-toxic dose of CPF on brain levels of 15-F2t-IsoP, a reliable and sensitive marker of in vivo oxidative stress, in mice, at birth and later stages corresponding to adolescence and adulthood. In addition to 15-F2t-IsoP, we evaluated the levels of PGE2, a lipid mediator involved in inflammatory and immune responses but also controlling several physiological and pathological functions in the brain and found altered in ASD.

Our findings indicate that at birth, BTBR mice, a validated model of idiopathic autism displaying both behavioral and immunological deficits associated with ASD, are characterized by increased brain levels of 15-F2t-IsoP as compared to C57 mice, a strain with strong gene background similarity but lacking of autism-like phenotype. Gestational exposure to CPF promoted the increase of both 15-F2t-IsoP and PGE2 in BTBR mice but not in C57 mice, suggesting an important strain-treatment interaction effect and a selective vulnerability of the BTBR strain to this organophosphate.

Interestingly, the increase in 15-F2t-IsoP and PGE2 levels occurred with different temporal patterns, being 15-F2t-IsoP elevated at birth and at weaning and PGE2 levels not affected at birth but stably increased at weaning and adulthood. This suggests that the occurrence of oxidative stress in a specific temporal window in early postnatal life might result in delayed permanent alterations in specific molecular pathways relevant to ASD, such as PGE2 signaling [6, 7, 17].

PGE2 is a major arachidonic acid metabolite, preferentially formed during the enzymatic activity of COX-2 [48], the inducible COX isoform. Besides its well-known role in inflammation, COX-2/PGE2 pathway plays important physiological functions. In normal brain, COX-2 is localized to excitatory glutamatergic neurons and its expression is dependent on synaptic activity [49]. COX-2 expression is developmental regulated and in the neocortex COX-2 positive neurons show a typical laminar distribution that is heavily disrupted in subjects affected by Rett syndrome, a neurodevelopmental disorder characterized by a defective development of cortical neurons [50]. It is tempting to speculate that the increased PGE2 levels after prenatal CPF exposure could reflect an exacerbation of the functional abnormalities evidenced in young/adult BTBR mice, which include widespread reductions in cortical thickness and reduced fronto-cortical metabolism [51].

In the only study measuring F2-IsoPs and PGE2 in rats acutely exposed to CPF, the former were positively associated with learning deficits induced by CPF [47].

Though the design of our present study does not allow a direct correlation between the altered brain levels of 15-F2t-IsoP and PGE2 and autism-like behavioral, it is worth noting that in the siblings of the experimental subjects used in the current study, CPF gestational exposure caused a significant delay in motor maturation in the first 2 weeks of life and worsened some of the autistic-like traits of BTBR mice at adulthood [25]. Anomalies in specific motor responses, common to other mouse models of ASD, have been proposed as early markers for ASD diagnosis [52–54] strengthening the relevance of our findings [25].

In light of the strong prevalence of ASD and other neurodevelopmental disorders in the male sex, it is worth considering that the effects of CPF are of comparable extent in the two sexes both at birth and at adulthood. However, on PND 21, there is a significant sex effect, with males displaying the highest levels of 15-F2t-IsoP and PGE2. This observation supports a higher vulnerability of males to prenatal exposure to CPF and is consistent with sex differences in CPF behavioral effects, suggested by epidemiological and experimental studies. Horton et al. [55] found that male children were more susceptible than females as for cognitive effects, and we reported higher vulnerability of the male sex to developmental CPF effects, particularly as far as early neonatal behavior patterns is concerned [25].

A higher vulnerability of the male sex to adverse conditions associated with oxidative stress is indicated by a study in newborn twins, a human natural model for studying fetal adaptation to suboptimal supply of nutrients—the most likely cause of reduced fetal growth—in which occurrence of oxidative stress has been demonstrated. In this study, males displayed higher cord blood levels of 15-F2t-IsoP than females; the observation held true when comparing twins of unlike-sex pairs, strongly suggesting that males are more exposed to oxidative stress than their sisters when experiencing the same “in utero” environmental challenge [56].

Strong evidence indicates that males are more sensitive to early-life challenges, such as infection and ischemia, and it has been suggested that this may contribute to sexual dimorphism evident in early-onset developmental disorders, including autism ([57] and references therein).

In line with our previous results [25], CPF-exposed BTBR mice showed a reduced somatic growth in the developmental phase. Both CPF-induced oxidative stress and reduced body weight reached the apex at weaning, to return to normal level at adulthood, suggesting that reduced growth rate and increased brain oxidative stress might be part of the same adaptive response to adverse conditions during early phases of life. Although the effect on somatic growth was similar in males and females, the increase in brain oxidative stress was higher in males, further supporting the particular vulnerability of the male sex to developmental suboptimal conditions. The reduction in body weight resulting from CPF treatment at PND 21 in BTBR mice was not associated with a corresponding reduction in brain weight, an observation that might suggest a transient increase in brain size with respect to body weight during the developmental phase. Interestingly, a transient brain overgrowth has been reported in some studies on ASD children ([58] and references therein), in which overgrowth and neural dysfunctions have been suggested to underlie some of the autistic symptoms.

Altogether, we show here that a widely diffused insecticide reportedly implicated in increased risk of neuromotor and neuropsychological impairments in children induces a transient up-regulation of an oxidative stress biomarker and a permanent alteration in PGE2 pathway in a validated mouse model of ASD. A possible limitation of our study is the use of a single CPF dose. Importantly, the CPF dose used in the current and previous studies is safe with respect to reproductive performance of treated dams (pregnancy length, number of pups at delivery, sex ratio); it does not induce systemic toxicity in dams or major effects on pup’s health, and it is below the threshold for inhibition of brain AChE in the mouse species [26, 59]. Our findings thus reinforce the growing evidence that the developmental neurotoxicity of CPF is not strictly or uniquely related to its anticholinesterase activity [60]. However, there is an indication of potential non-monotonic dose-response effects of gestational CPF on behavioral functions [61]. Even within the range of sub-toxic doses, in critical maturational phase, “higher” doses may cause small and transient enhancement of cholinergic neurotransmission: such event might be paradoxically protective for the developing brain insofar that compensatory mechanisms are called upon. Thus, we cannot exclude that a lower dose of CPF would have evidenced a different pattern of effects in the two strains considered.

Conclusions

In the last years, numerous studies have suggested that immunological factors as well as increased susceptibility to oxidative stress may contribute to the etiology of ASD.

Our findings indicate that prenatal CPF exposure, at a dose devoid of maternal or systemic toxicity, significantly affects oxidative stress and PGE2 synthesis in a validated mouse model of idiopathic autism. In the light of the hypothesis associating oxidative stress, lipid metabolism alterations, and ASD etiology in children, future work in this mouse model of autism will help in elucidating the mechanistic pathways linking exposure to a widely diffused neurotoxicant such as CPF, neurodevelopment alterations, and behavioral deficits.

Abbreviations

15-F2t-IsoP, 15-F2t-Isoprostane; AChE, acetylcholinesterase; ASD, autism spectrum disorders; BTBR, black and tan brachyury T+tf/J; C57, C57BL6/J; CPF, chlorpyrifos; COX, cyclooxygenase; GD, gestational day; OP, organophosphate; PG, prostaglandin; PGE2, prostaglandin E2; PND, postnatal day; PUFAs, polyunsaturated fatty acids.

References

  1. Faustman EM, Silbernagel SM, Fenske RA, Burbacher TM, Ponce RA. Mechanisms underlying children’s susceptibility to environmental toxicants. Environ Health Perspect. 2000;108(Suppl):13–21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Kalkbrenner AE, Daniels JL, Chen JC, Poole C, Emch M, Morrissey J. Perinatal exposure to hazardous air pollutants and autism spectrum disorders at age 8. Epidemiology. 2010;21:631–41.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Pardo CA, Vargas DL, Zimmerman AW. Immunity, neuroglia and neuroinflammation in autism. Int Rev Psychiatry. 2005;17:485–95.

    Article  PubMed  Google Scholar 

  4. Chauhan A, Chauhan V, Brown WT, Cohen I. Oxidative stress in autism: increased lipid peroxidation and reduced serum levels of ceruloplasmin and transferrin—the antioxidant proteins. Life Sci. 2004;75:2539–49.

    Article  CAS  PubMed  Google Scholar 

  5. Onore C, Careaga M, Ashwood P. The role of immune dysfunction in the pathophysiology of autism. Brain, Behavior, and Immunity. 2012;383–392.

  6. Wong CT, Wais J, Crawford DA. Prenatal exposure to common environmental factors affects brain lipids and increases risk of developing autism spectrum disorders. Eur J Neurosci. 2015;42:2742–60.

    Article  PubMed  Google Scholar 

  7. Tamiji J, Crawford DA. The neurobiology of lipid metabolism in autism spectrum disorders. Neurosignals. 2010;18:98–112.

    Article  CAS  PubMed  Google Scholar 

  8. Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine. Volume 10. 2007.

    Google Scholar 

  9. Terry AV. Functional consequences of repeated organophosphate exposure: potential non-cholinergic mechanisms. Pharmacology and Therapeutics. 2012;355–365.

  10. Soltaninejad K, Abdollahi M. Current opinion on the science of organophosphate pesticides and toxic stress: a systematic review. Med Sci Monit. 2009;15:RA75–A90.

    CAS  PubMed  Google Scholar 

  11. Farina M, Avila DS, Da Rocha JBT, Aschner M. Metals, oxidative stress and neurodegeneration: a focus on iron, manganese and mercury. Neurochemistry International. 2013;575–594.

  12. Bagchi D, Hassoun EA, Bagchi M, Stohs SJ. Chromium-induced excretion of urinary lipid metabolites, DNA damage, nitric oxide production, and generation of reactive oxygen species in Sprague-Dawley rats. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1995;110:177–87.

    Article  CAS  PubMed  Google Scholar 

  13. Bazinet RP, Layé S. Polyunsaturated fatty acids and their metabolites in brain function and disease. Nat Rev Neurosci. 2014;15:771–85.

    Article  CAS  PubMed  Google Scholar 

  14. Minghetti L. Cyclooxygenase-2 (COX-2) in inflammatory and degenerative brain diseases. J Neuropathol Exp Neurol. 2004;63:901–10.

    Article  CAS  PubMed  Google Scholar 

  15. Roberts LJ, Fessel JP, Davies SS. The biochemistry of the isoprostane, neuroprostane, and isofuran pathways of lipid peroxidation. Brain Pathol. 2005;15:143–8.

    Article  CAS  PubMed  Google Scholar 

  16. Ming X, Stein TP, Brimacombe M, Johnson WG, Lambert GH, Wagner GC. Increased excretion of a lipid peroxidation biomarker in autism. Prostaglandins Leukot Essent Fat Acids. 2005;73:379–84.

    Article  CAS  Google Scholar 

  17. El-Ansary A, Al-Ayadhi L. Lipid mediators in plasma of autism spectrum disorders. Lipids Health Dis. 2012;11:160.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. McFarlane HG, Kusek GK, Yang M, Phoenix JL, Bolivar VJ, Crawley JN. Autism-like behavioral phenotypes in BTBR T+tf/J mice. Genes, Brain Behav. 2008;7:152–63.

    Article  CAS  Google Scholar 

  19. Jones-Davis DM, Yang M, Rider E, Osbun NC, da Gente GJ, Li J, Katz AM, Weber MD, Sen S, Crawley J, Sherr EH. Quantitative trait loci for interhemispheric commissure development and social behaviors in the BTBR T+ tf/J mouse model of autism. PLoS One. 2013;8.

  20. Yang M, Abrams DN, Zhang JY, Weber MD, Katz AM, Clarke AM, Silverman JL, Crawley JN. Low sociability in BTBR T+tf/J mice is independent of partner strain. Physiol Behav. 2012;107:649–62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Moy SS, Nadler JJ, Young NB, Perez A, Holloway LP, Barbaro RP, Barbaro JR, Wilson LM, Threadgill DW, Lauder JM, Magnuson TR, Crawley JN. Mouse behavioral tasks relevant to autism: phenotypes of 10 inbred strains. Behav Brain Res. 2007;176:4–20.

    Article  PubMed  Google Scholar 

  22. Heo Y, Zhang Y, Gao D, Miller VM, Lawrence DA. Aberrant immune responses in a mouse with behavioral disorders. PLoS One. 2011;6.

  23. Careaga M, Schwartzer J, Ashwood P. Inflammatory profiles in the BTBR mouse: how relevant are they to autism spectrum disorders? Brain Behav Immun. 2015;43:11–6.

    Article  CAS  PubMed  Google Scholar 

  24. Kern JK, Jones AM. Evidence of toxicity, oxidative stress, and neuronal insult in autism. J Toxicol Environ Health B Crit Rev. 2006;9:485–99.

    Article  CAS  PubMed  Google Scholar 

  25. De Felice A, Scattoni ML, Ricceri L, Calamandrei G. Prenatal exposure to a common organophosphate insecticide delays motor development in a mouse model of idiopathic autism. PLoS One. 2015;10:e0121663.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Venerosi A, Ricceri L, Scattoni ML, Calamandrei G. Prenatal chlorpyrifos exposure alters motor behavior and ultrasonic vocalization in CD-1 mouse pups. Environ Health. 2009;8:12.

    Article  PubMed  PubMed Central  Google Scholar 

  27. Grandjean P, Landrigan PJ. Neurobehavioural effects of developmental toxicity. The Lancet Neurology. 2014;330–338.

  28. Engel SM, Wetmur J, Chen J, Zhu C, Barr DB, Canfield RL, Wolff MS. Prenatal exposure to organophosphates, paraoxonase 1, and cognitive development in childhood. Environ Health Perspect. 2011;119:1182–8.

    Article  PubMed  PubMed Central  Google Scholar 

  29. Eskenazi B, Marks AR, Bradman A, Harley K, Barr DB, Johnson C, Morga N, Jewell NP. Organophosphate pesticide exposure and neurodevelopment in young Mexican-American children. Environ Health Perspect. 2007;115:792–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Rauh V, Arunajadai S, Horton M, Perera F, Hoepner L, Barr DB, Whyatt R. Seven-year neurodevelopmental scores and prenatal exposure to chlorpyrifos, a common agricultural pesticide. Environ Health Perspect. 2011;119:1196–201.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Eaton DL, Daroff RB, Autrup H, Bridges J, Buffler P, Costa LG, Coyle J, McKhann G, Mobley WC, Nadel L, Neubert D, Schulte-Hermann R, Spencer PS. Review of the toxicology of chlorpyrifos with an emphasis on human exposure and neurodevelopment. Crit Rev Toxicol. 2008;38 Suppl 2:1–125.

    Article  CAS  PubMed  Google Scholar 

  32. Slotkin TA, Seidler FJ. Prenatal chlorpyrifos exposure elicits presynaptic serotonergic and dopaminergic hyperactivity at adolescence: critical periods for regional and sex-selective effects. Reprod Toxicol. 2007;23:421–7.

    Article  CAS  PubMed  Google Scholar 

  33. Tait S, Ricceri L, Venerosi A, Maranghi F, Mantovani A, Calamandrei G. Long-term effects on hypothalamic neuropeptides after developmental exposure to chlorpyrifos in mice. Environ Health Perspect. 2009;117:112–6.

    Article  CAS  PubMed  Google Scholar 

  34. Venerosi A, Ricceri L, Rungi A, Sanghez V, Calamandrei G. Gestational exposure to the organophosphate chlorpyrifos alters social-emotional behaviour and impairs responsiveness to the serotonin transporter inhibitor fluvoxamine in mice. Psychopharmacology (Berl). 2010;208:99–107.

    Article  CAS  Google Scholar 

  35. Venerosi A, Ricceri L, Tait S, Calamandrei G. Sex dimorphic behaviors as markers of neuroendocrine disruption by environmental chemicals: the case of chlorpyrifos. Neurotoxicology. 2012;33:1420–6.

    Article  CAS  PubMed  Google Scholar 

  36. Ricceri L, Venerosi A, Capone F, Cometa MF, Lorenzini P, Fortuna S, Calamandrei G. Developmental neurotoxicity of organophosphorous pesticides: fetal and neonatal exposure to chlorpyrifos alters sex-specific behaviors at adulthood in mice. Toxicol Sci. 2006;93:105–13.

    Article  CAS  PubMed  Google Scholar 

  37. De Felice A, Venerosi A, Ricceri L, Sabbioni M, Scattoni ML, Chiarotti F, Calamandrei G. Sex-dimorphic effects of gestational exposure to the organophosphate insecticide chlorpyrifos on social investigation in mice. Neurotoxicol Teratol. 2014;46:32–9.

    Article  PubMed  Google Scholar 

  38. Minghetti L, Greco A, Cardone F, Puopolo M, Ladogana A, Almonti S, Cunningham C, Perry VH, Pocchiari M, Levi G. Increased brain synthesis of prostaglandin E2 and F2-isoprostane in human and experimental transmissible spongiform encephalopathies. J Neuropathol Exp Neurol. 2000;59:866–71.

    Article  CAS  PubMed  Google Scholar 

  39. Benno R, Smirnova Y, Vera S, Liggett A, Schanz N. Exaggerated responses to stress in the BTBR T+tf/J mouse: an unusual behavioral phenotype. Behav Brain Res. 2009;197:462–5.

    Article  CAS  PubMed  Google Scholar 

  40. Andersen SL. Trajectories of brain development: point of vulnerability or window of opportunity? Neurosci Biobehav Rev. 2003;27:3–18.

    Article  PubMed  Google Scholar 

  41. Laviola G, Adriani W, Morley-Fletcher S, Terranova ML. Peculiar response of adolescent mice to acute and chronic stress and to amphetamine: evidence of sex differences. Behav Brain Res. 2002;130:117–25.

    Article  CAS  PubMed  Google Scholar 

  42. RG. W: New Statistical Procedures for the Social Sciences. Modern sol. Lawrence Erlbaum; 1987

  43. Łukaszewicz-Hussain A. Liver and serum glutathione concentration and liver hydrogen peroxide in rats subchronically intoxicated with chlorfenvinphos—organophosphate insecticide. Med Pr. 2011;62:23–9.

    PubMed  Google Scholar 

  44. Ambali SF, Idris SB, Onukak C, Shittu M, Ayo JO. Ameliorative effects of vitamin C on short-term sensorimotor and cognitive changes induced by acute chlorpyrifos exposure in Wistar rats. Toxicol Ind Heal. 2010;26:547–58.

    Article  CAS  Google Scholar 

  45. Gultekin F, Delibas N, Yasar S, Kilinc I. In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol. 2001;75:88–96.

    Article  CAS  PubMed  Google Scholar 

  46. Gupta SC, Mishra M, Sharma A, Deepak Balaji TGR, Kumar R, Mishra RK, Chowdhuri DK. Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species. Ecotoxicol Environ Saf. 2010;73:1415–23.

    Article  CAS  PubMed  Google Scholar 

  47. López-Granero C, Cañadas F, Cardona D, Yu Y, Giménez E, Lozano R, Avila DS, Aschner M, Sánchez-Santed F. Chlorpyrifos-, diisopropylphosphorofluoridate-, and parathion-induced behavioral and oxidative stress effects: are they mediated by analogous mechanisms of action? Toxicol Sci. 2013;131:206–16.

    Article  PubMed  Google Scholar 

  48. Bosetti F, Langenbach R, Weerasinghe GR. Prostaglandin E2 and microsomal prostaglandin E synthase-2 expression are decreased in the cyclooxygenase-2-deficient mouse brain despite compensatory induction of cyclooxygenase-1 and Ca2+-dependent phospholipase A2. J Neurochem. 2004;91:1389–97.

    Article  CAS  PubMed  Google Scholar 

  49. Kaufmann WE, Worley PF, Pegg J, Bremer M, Isakson P. COX-2, a synaptically induced enzyme, is expressed by excitatory neurons at postsynaptic sites in rat cerebral cortex. Proc Natl Acad Sci U S A. 1996;93:2317–21.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  50. Kaufmann WE, Worley PF, Taylor CV, Bremer M, Isakson PC. Cyclooxygenase-2 expression during rat neocortical development and in Rett syndrome. Brain Dev. 1997;19:25–34.

    Article  CAS  PubMed  Google Scholar 

  51. Dodero L, Damiano M, Galbusera A, Bifone A, Tsaftsaris SA, Scattoni ML, Gozzi A. Neuroimaging evidence of major morpho-anatomical and functional abnormalities in the BTBR T+TF/J mouse model of autism. PLoS One. 2013;8.

  52. Leonard HC, Bedford R, Charman T, Elsabbagh M, Johnson MH, Hill EL. Motor development in children at risk of autism: a follow-up study of infant siblings. Autism. 2014;18:281–91.

    Article  PubMed  Google Scholar 

  53. Shakiba A. The role of the cerebellum in neurobiology of psychiatric disorders. Neurol Clin. 2014;32:1105–15.

    Article  PubMed  Google Scholar 

  54. Teitelbaum P, Teitelbaum O, Nye J, Fryman J, Maurer RG. Movement analysis in infancy may be useful for early diagnosis of autism. Proc Natl Acad Sci U S A. 1998;95:13982–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  55. Horton MK, Kahn LG, Perera F, Barr DB, Rauh V. Does the home environment and the sex of the child modify the adverse effects of prenatal exposure to chlorpyrifos on child working memory? Neurotoxicol Teratol. 2012;34:534–41.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  56. Minghetti L, Greco A, Zanardo V, Suppiej A. Early-life sex-dependent vulnerability to oxidative stress: the natural twining model. J Matern Fetal Neonatal Med. 2013;26:259–62.

    Article  CAS  PubMed  Google Scholar 

  57. Schwarz JM, Bilbo SD. Sex, glia, and development: interactions in health and disease. Hormones and Behavior. 2012;243–253.

  58. Courchesne E, Mouton PR, Calhoun ME, Semendeferi K, Ahrens-Barbeau C, Hallet MJ, Barnes CC, Pierce K. Neuron number and size in prefrontal cortex of children with autism. JAMA. 2011;306:2001–10.

    Article  CAS  PubMed  Google Scholar 

  59. Cole TB, Li WF, Co AL, Hay AM, MacDonald JW, Bammler TK, Farin FM, Costa LG, Furlong CE. Repeated gestational exposure of mice to chlorpyrifos oxon is associated with paraoxonase 1 (PON1) modulated effects in maternal and fetal tissues. Toxicol Sci. 2014;141:409–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  60. Slotkin TASF. Oxidative and excitatory mechanisms of developmental neurotoxicity: transcriptional profiles for chlorpyrifos, diazinon, dieldrin, and divalent nickel in PC12 cells. Environ Health Perspect. 2009;117:587–96.

    Article  CAS  PubMed  Google Scholar 

  61. Levin ED, Addy N, Baruah A, Elias A, Christopher NC, Seidler FJ, Slotkin TA. Prenatal chlorpyrifos exposure in rats causes persistent behavioral alterations. Neurotoxicol Teratol. 2002;24:733–41.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We acknowledge the support of Flavia Chiarotti for the statistical advices, of Maria Carla Giorgi and Laura Ricceri in tissue samples’ collection, and of Luigia Cancemi in maintaining the mouse colony at the ISS.

Funding

This work was supported by Istituto Superiore di Sanità, ISS Research grant 13CAL and by EU 7th framework programme for research, FP7 HEALS Grant No. 603946.

Availability of data and materials

There is no data, software, databases, and application/tool available apart from the reported in the present study. Data supporting the conclusions are presented in the manuscript.

Authors’ contributions

ADF designed and performed the animal experiments, carried out the statistical analysis, and drafted the manuscript. AG carried out the biochemical assays and contributed to the data analysis. GC conceived the study, participated in the design of animal experiments and data analysis, and edited the manuscript. LM conceived of the study, participated in its design and coordination, performed data analysis, and edited the manuscript. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval and consent to participate

This study was carried out in accordance with the Italian Animal Welfare legislation (D.L. 26/2014) that implemented the European Committee Council Directive (2010/63/EEC). The Italian Ministry of Health specifically approved the protocol of this study on 10/31/2011, Authorization no. 223/2011-B to G.C.

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Authors and Affiliations

  1. Section of Neurotoxicology and Neuroendocrinology, Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, I-00161, Rome, Italy

    Alessia De Felice & Gemma Calamandrei

  2. Section of Experimental Neurology, Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, I-00161, Rome, Italy

    Anita Greco & Luisa Minghetti

  3. Present address: Center for Neuroscience and Cognitive Systems @UniTn, Istituto Italiano di Tecnologia, Via Bettini 31, 38068, Rovereto (TN), Italy

    Alessia De Felice

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  1. Alessia De Felice
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  2. Anita Greco
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  4. Luisa Minghetti
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Correspondence to Luisa Minghetti.

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De Felice, A., Greco, A., Calamandrei, G. et al. Prenatal exposure to the organophosphate insecticide chlorpyrifos enhances brain oxidative stress and prostaglandin E2 synthesis in a mouse model of idiopathic autism. J Neuroinflammation 13, 149 (2016). https://doi.org/10.1186/s12974-016-0617-4

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Keywords

Journal of Neuroinflammation

ISSN: 1742-2094