Segmentation and visualization of membranes and intracellular organelles contours in 3D electron microscopy

Christophe Bron; F. Sadlo; Gabor Szekely; W. Neuenschwander; Olaf Kuebler; Joerg Schuepbach

doi:10.1117/12.185232

9 September 1994 Segmentation and visualization of membranes and intracellular organelles contours in 3D electron microscopy

Christophe Bron, F. Sadlo, Gabor Szekely, W. Neuenschwander, Olaf Kuebler, Joerg Schuepbach

Author Affiliations +

Proceedings Volume 2359, Visualization in Biomedical Computing 1994; (1994) https://doi.org/10.1117/12.185232
Event: Visualization in Biomedical Computing 1994, 1994, Rochester, MN, United States

Abstract

Transmission Electron Microscopy allows the visualisation of cell organelles and intracellular structures at high magnification. But this technique is physically limited to ultrathin sections of about 20 to 100 nm because the electrons have to get through the biological structures to image them. In order to reconstruct the third dimension, we have previously developed a method based on laser topographical references to correct the physical deformations linked with the sectioning process and to allow the fine registration of the images1. The volume information is reconstructed but the intracellular organelles which are limited by membranes, are still embedded in a matrix of opaque cytoplasm containing dense ribosomes and fibrils. The rendering of the surface, volume and the original 3D appearance of each organelle requires individual segmentation. This operation is presently the only conceivable way to visualise the internal organisation of cells. We expose here some methods and algorithms for extraction of organellescontours and their subsequent preliminary visualisation. The algorithms allow the representation of the internal cell structure and pave the way toward virtual immersion.

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Christophe Bron, F. Sadlo, Gabor Szekely, W. Neuenschwander, Olaf Kuebler, and Joerg Schuepbach "Segmentation and visualization of membranes and intracellular organelles contours in 3D electron microscopy", Proc. SPIE 2359, Visualization in Biomedical Computing 1994, (9 September 1994); https://doi.org/10.1117/12.185232

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KEYWORDS

Visualization

Image segmentation

Electron microscopy

3D modeling

3D image processing

Data acquisition

Image processing

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