A new approach for sustained and efficient H2 photoproduction by Chlamydomonas reinhardtii†
Abstract
Sustained H2 photoproduction is demonstrated in green algae under a train of strong white light pulses interrupted by longer dark phases. The devised protocol relies on the presence of the [FeFe]-hydrogenase in algal chloroplasts, which is activated within a few seconds after the establishment of anaerobiosis. H2 photoproduction proceeds for up to 3 days with the maximum rate occurring in the first 6 hours.