biologia plantarum

International journal on Plant Life established by Bohumil N�mec in 1959

Biologia plantarum 45:593-596, 2002 | DOI: 10.1023/A:1022390917656

Influence of Brassinosteroids on Antioxidant Enzymes Activity in Tomato Under Different Temperatures

L.M. Mazorra1, M. N�ñez1, M. Hechavarria2, F. Coll2, M.J. S�nchez-Blanco3
1 Dpto. Fisiologia y Bioqu�mica Vegetal, Instituto Nacional de Ciencias Agr�colas (INCA), La Habana, Cuba
2 Laboratorio de Productos Naturales, Facultad de Qu�mica, Universidad de La Habana, La Habana, Cuba
3 Dpto. Riego y Salinidad, Centro de Edafolog�a y Biolog�a Aplicada del Segura (CSIC), Murcia, Spain

The effect of brassinosteroids (BRs) on catalase (EC 1.11.1.6), peroxidase (EC 1.11.1.7) and superoxide dismutase (SOD, EC 1.15.1.1) activity in tomato leaf discs was analyzed at 25 and 40 �C. Tomato leaf discs were preincubated for 24 h in Petri dishes with 24-epibrassinolide (EBR) or a polyhydroxylated spirostanic analogue of brassinosteroids (MH5). Both concentrations (10.60 and 2.12 nM) of EBR and MH5 stimulated the activity of SOD at 25 and 40 �C, the MH5-stimulated increase of this enzyme activity was greater. Peroxidase activity was unaffected at 25 �C, while at 40 �C this activity was enhanced by both compounds. The changes in catalase activity markedly depended on the structure BRs, doses and temperature. The results suggest a possible role of EBR and MH5 in the reduction of cell damage produced by heat stress due to induction of enzymatic antioxidants.

Keywords: brassinosteroid analogue; catalase; heat stress; Lycopersicon esculentum; peroxidase; superoxide dismutase
Subjects: antioxidants; brassinosteroids, antioxidant enzymes; catalase; heat stress, brassinosteroids, antioxidant enzymes; Lycopersicon esculentum; peroxidase; superoxide dismutase, brassinosteroids; temperature, brassinosteroids, antioxidant enzymes; tomato, brassinosteroids, antioxidant enzymes

Published: December 1, 2002  Show citation

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Mazorra, L.M., N�ñez, M., Hechavarria, M., Coll, F., & S�nchez-Blanco, M.J. (2002). Influence of Brassinosteroids on Antioxidant Enzymes Activity in Tomato Under Different Temperatures. Biologia plantarum,�45(4),�593-596. doi:�10.1023/A:1022390917656
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    References

    1. Aebi, H.: Catalase in vitro.-Methods Enzymol. 105: 121-126, 1984. Go to original source...
    2. Bergmeyer, H.U.: Peroxidase.-In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 685-690. Academic Press, New York-London 1974.
    3. Beyer, W.F., Fridovich, I.: Assaying for superoxide dismutase activity: Some large consequences of minor changes in conditions.-Anal. Biochem. 161: 559-566, 1987. Go to original source...
    4. Clouse, S.D., Sasse, J.M.: Brassinosteroids: Essential regulators of plant growth and development.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 427-451, 1998. Go to original source...
    5. Coll, M.F., Jomarr�n, R.I.M., Robaina, R.C.M., Alonso, B.E.M., Cabrera, P.M.T.: Polyhydroxyspirostanones as plant growth regulators.-PCT Int. Appl. W09713. 780. 1995.
    6. Chen, S.N., Li, J.N., You, H.L., Zhu, H.J., Qin, Z.B., Hong, G.M., Shen, Y.G.: The effect of a compound inducing cold resistance and homobrassinolide on the chilling resistance of plateau rice.-Acta bot. yunnanica 19: 184-190, 1997.
    7. Dat, J.F., Lopez-Delgado, H., Foyer, C.H., Scott, I.M.: Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings.-Plant Physiol. 116: 1351-1357, 1998. Go to original source...
    8. Dhaubhadel, S., Chaudhary, S., Dobinson, K.F., Krishna, P.: Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings.-Plant mol. Biol. 40: 333-342, 1999. Go to original source...
    9. Foyer, C.H., Lopez-Delgado, H., Dat, J.F., Scott, I.M.: Hydrogen peroxide-and gluthatione-associated mechanisms of acclimatory stress tolerance and signalling.-Physiol. Plant. 100: 241-254, 1997. Go to original source...
    10. Hathout, T.A.: Salinity stress and its counteraction by the growth regulator "Brassinolide" in wheat plants (Triticum aestivum L. cultivar Giza 157).-Egyptian J. physiol. Sci. 20: 127-152, 1996.
    11. Hern�ndez, J.A., Jim�nez, A., Mullineaux, P., Sevilla, F. Tolerance of pea (Pisum sativum L.) to long-term salt stress in associated with induction of antioxidant defences.-Plant Cell Environ. 23: 853-862, 2000. Go to original source...
    12. Iglesias, A.M.A., Lara, V.L., Martinez, C.P., Manchado, F.C.: Synthesis of spirobrassinosteroid analogs of 6-deoxocastasterone.-Quim. Nova 20: 361-364, 1997. Go to original source...
    13. Iglesias, A.M.A., Perez, G.R., Coll, M.F.: Natural brassinosteroids and their synthetic analogs.-Rev. CENIC Cienc. Quim. 27: 3-12, 1996.
    14. Ikekawa, N., Zhao, Y.J.: Application of 24-epibrassinolide in agriculture.-In: Cutler, H.G., Yokota, T., Adam, G. (ed.): Brassinosteroids: Chemistry, Bioactivity and Applications. Pp. 280-291. American Chemical Society, Washington 1991. Go to original source...
    15. Jomarr�n, I.: Synthesis of biologically active spirostanones and spirostanlactones.-Thesis. Faculty of Chemistry, University of Havana, Havana 1995.
    16. Kulaeva, O.N., Burkhanova, E.A., Fedina, A.B., Kaokhlova, V.A., Bokebayeva, G.A.: Effect of brassinosteroids on protein synthesis and plant-cell-ultrastructure under stress conditions.-In: Cutler, H.G., Yokota, T., Adam, G. (ed.): Brassinosteroids: Chemistry, Bioactivity and Applications. Pp. 141-155. American Chemical Society, Washington 1991. Go to original source...
    17. Li, L, Van Staden, J.: Effects of plant growth regulators on the antioxidant system in seedlings of two maize cultivars subjected to water stress.-Plant Growth Regul. 25: 81-87, 1998. Go to original source...
    18. Li, L., Van Staden, J., Jaker, A.K.: Effects of plant growth regulators on drought resistance of two maize cultivars.-South afr. J. Bot. 64: 116-120, 1998. Go to original source...
    19. Ramirez, J.A., Centurion, O.M.T., Gros, E.G., Galagovsky, L.R.: Synthesis and bioactivity evaluation of brassinosteroid analogs.-Steroids 65: 329-337, 2000. Go to original source...
    20. Sabehat, A., Weiss, D., Lurie, S.: Heat-shock proteins and cross tolerance in plants.-Physiol. Plant. 103: 437-441, 1998. Go to original source...
    21. Vardhini, B.V., Rao, S.S.R.: Effect of brassinosteroids on salinity induced growth inhibition of groundnut seedlings.-Indian J. Plant Physiol. 2: 156-157, 1997.
    22. Wilen, R.W., Sacco, M., Gusta, L.V., Krishna, P.: Effects of 24-epibrassinolide on freezing and thermotolerance of bromegrass (Bromus inermis) cell cultures.-Physiol. Plant. 95: 195-202, 1995. Go to original source...
    23. Zhu, C., Zeng, G.-W., Liu, F.: Effect of epi-brassinolide on the heat shock tolerance and antioxidant metabolism in cucumber seedling.-J. Zhejiang agr. Univ. 22: 284-288, 1996.

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