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Leptospira in livestock in Madagascar: uncultured strains, mixed infections and small mammal-livestock transmission highlight challenges in controlling and diagnosing leptospirosis in the developing world

Published online by Cambridge University Press:  26 September 2019

Soanandrasana Rahelinirina ,
Mark H. Moseley Open the ORCID record for Mark H. Moseley [Opens in a new window] ,
Kathryn J. Allan ,
Emmanuel Ramanohizakandrainy ,
Sati Ravaoarinoro ,
Minoarisoa Rajerison ,
Vincent Rakotoharinome  and
Sandra Telfer
Soanandrasana Rahelinirina
Affiliation:
Plague Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Mark H. Moseley*
Affiliation:
School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, UK
Kathryn J. Allan
Affiliation:
Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, University Avenue, Glasgow, G12 8QQ, UK
Emmanuel Ramanohizakandrainy
Affiliation:
University of Antananarivo, BP 566, Ankatso, 101 Antananarivo, Madagascar
Sati Ravaoarinoro
Affiliation:
University of Antananarivo, BP 566, Ankatso, 101 Antananarivo, Madagascar
Minoarisoa Rajerison
Affiliation:
Plague Unit, Institut Pasteur de Madagascar, 101 Antananarivo, Madagascar
Vincent Rakotoharinome
Affiliation:
Ministere de l'Agriculture et de l'Elevage, BP 1453, Analakely, Antananarivo, Madagascar
Sandra Telfer
Affiliation:
School of Biological Sciences, University of Aberdeen, Aberdeen, AB24 2TZ, UK
*
Author for correspondence: Mark H. Moseley, E-mail: mark.moseley@abdn.ac.uk
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Abstract

In developing countries, estimates of the prevalence and diversity of Leptospira infections in livestock, an important but neglected zoonotic pathogen and cause of livestock productivity loss, are lacking. In Madagascar, abattoir sampling of cattle and pigs demonstrated a prevalence of infection of 20% in cattle and 5% in pigs by real-time PCR. In cattle, amplification and sequencing of the Leptospira-specific lfb1 gene revealed novel genotypes, mixed infections of two or more Leptospira species and evidence for potential transmission between small mammals and cattle. Sequencing of the secY gene demonstrated genetic similarities between Leptospira detected in Madagascar and, as yet, uncultured Leptospira strains identified in Tanzania, Reunion and Brazil. Detection of Leptospira DNA in the same animal was more likely in urine samples or pooled samples from four kidney lobes relative to samples collected from a single kidney lobe, suggesting an effect of sampling method on detection. In pigs, no molecular typing of positive samples was possible. Further research into the epidemiology of livestock leptospirosis in developing countries is needed to inform efforts to reduce human infections and to improve livestock productivity.

Keywords

AfricaMadagascarpathogenspilloverspirochaeteTanzaniaveterinaryzoonosis
Type
Research Article
Information
Parasitology , Volume 146 , Special Issue 14: BSP 2018 Autumn Symposium , December 2019 , pp. 1707 - 1713
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Copyright
Copyright © Cambridge University Press 2019 

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Footnotes

*

These first authors contributed equally to this article.

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